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http://hdl.handle.net/1942/29853
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DC Field | Value | Language |
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dc.contributor.author | Verstockt, Sare | - |
dc.contributor.author | De Hertogh, Gert | - |
dc.contributor.author | Van der Goten, Jan | - |
dc.contributor.author | Verstockt, Bram | - |
dc.contributor.author | Vancamelbeke, Maaike | - |
dc.contributor.author | Machiels, Kathleen | - |
dc.contributor.author | Van Lommel, Leentje | - |
dc.contributor.author | Schuit, Frans | - |
dc.contributor.author | Van Assche, Gert | - |
dc.contributor.author | Rutgeerts, Paul | - |
dc.contributor.author | Ferrante, Marc | - |
dc.contributor.author | Vermeire, Severine | - |
dc.contributor.author | ARIJS, Ingrid | - |
dc.contributor.author | Cleynen, Isabelle | - |
dc.date.accessioned | 2019-10-28T08:17:44Z | - |
dc.date.available | 2019-10-28T08:17:44Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | JOURNAL OF CROHNS & COLITIS, 13(7), p. 916-930 | - |
dc.identifier.issn | 1873-9946 | - |
dc.identifier.uri | http://hdl.handle.net/1942/29853 | - |
dc.description.abstract | Background and Aims: Early treatment of Crohn's disease [CD] is required in order to optimize patient outcomes. To this end, we need to gain a better understanding of the molecular changes at the onset of CD. Methods: As a model for the earliest mucosal CD lesions, we study post-operative recurrent CD [Rutgeerts score >= i2b]. We are the first to analyse gene and microRNA [miRNA] expression profiles in ileal biopsies from these patients, and compare them with those of newly diagnosed [<= 18 months] and late-stage [> 10 years after diagnosis] CD patients. Results: Except for one gene [WNT5A], there are no differential genes in CD patients without postoperative recurrence [i0], showing that previous disease did not influence gene expression in the neoterminal ileum, and that this model can be used to study early mucosal CD lesions. Gene expression and co-expression network dysregulation is more pronounced in newly diagnosed and late-stage CD than in post-operative recurrent CD, with most important modules associated with [a] granulocyte adhesion/diapedesis, and cholesterol biosynthesis. In contrast, we found a role for snoRNAs/miRNAs in recurrent CD, highlighting the potential importance of regulatory RNAs in early disease stages. Immunohistochemistry confirmed the expression of key dysregulated genes in damaged/regenerating epithelium and immune cells in recurrent CD. Conclusions: Aside from regulatory RNAs, there are no clear gene signatures separating postoperative recurrent, newly diagnosed, and late-stage CD. The relative contribution of dysregulated genes and networks differs, and suggests that surgery may reset the disease at the mucosal site, and therefore post-operative recurrent CD might be a good model a good model to study to study early mucosal CD lesions. | - |
dc.description.sponsorship | This work was supported by the Research Foundation Flanders [FWO] [no. G.0440.06, no. G.0582.08 and no. G.0479.10]. BV is a doctoral fellow, KM is a postdoctoral fellow and GVA, MF and SV are senior clinical investigators of the FWO. | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.rights | 2019 European Crohn’s and Colitis Organisation (ECCO). Published by Oxford University Press. All rights reserved. | - |
dc.subject.other | Early Crohn's disease; gene expression; gene regulation; molecular mechanisms | - |
dc.subject.other | early Crohn’s disease; gene expression; gene regulation; molecular mechanisms | - |
dc.title | Gene and Mirna Regulatory Networks During Different Stages of Crohn's Disease | - |
dc.type | Journal Contribution | - |
local.bibliographicCitation.conferencedate | 2017 | - |
local.bibliographicCitation.conferencename | ASHG Meeting | - |
local.bibliographicCitation.conferenceplace | Orlando, FL | - |
dc.identifier.epage | 930 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 916 | - |
dc.identifier.volume | 13 | - |
local.format.pages | 15 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Verstockt, Sare; Cleynen, Isabelle] Katholieke Univ Leuven, Dept Human Genet, Lab Complex Genet, O&N4 Herestr 49,Box 610, B-3000 Leuven, Belgium. [De Hertogh, Gert] Univ Hosp, Dept Morphol & Mol Pathol, Leuven, Belgium. [De Hertogh, Gert] Katholieke Univ Leuven, Dept Imaging & Pathol, Translat Cell & Tissue Res, Leuven, Belgium. [Van der Goten, Jan; Verstockt, Bram; Vancamelbeke, Maaike; Machiels, Kathleen; Van Assche, Gert; Rutgeerts, Paul; Ferrante, Marc; Vermeire, Severine; Arijs, Ingrid] Katholieke Univ Leuven, Dept Chron Dis Metab & Aging CHROMETA, Translat Res Ctr Gastrointestinal Disorders TARGI, Leuven, Belgium. [Verstockt, Bram; Van Assche, Gert; Rutgeerts, Paul; Ferrante, Marc; Vermeire, Severine] Katholieke Univ Leuven, Univ Hosp, Dept Gastroenterol & Hepatol, Leuven, Belgium. [Van Lommel, Leentje; Schuit, Frans] Katholieke Univ Leuven, Dept Cellular & Mol Med, Gene Express Unit, Leuven, Belgium. [Arijs, Ingrid] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. [Arijs, Ingrid] Jessa Hosp, Hasselt, Belgium. | - |
local.publisher.place | OXFORD | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1093/ecco-jcc/jjz007 | - |
dc.identifier.isi | 000485146200012 | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
item.contributor | Verstockt, Sare | - |
item.contributor | De Hertogh, Gert | - |
item.contributor | Van der Goten, Jan | - |
item.contributor | Verstockt, Bram | - |
item.contributor | Vancamelbeke, Maaike | - |
item.contributor | Machiels, Kathleen | - |
item.contributor | Van Lommel, Leentje | - |
item.contributor | Schuit, Frans | - |
item.contributor | Van Assche, Gert | - |
item.contributor | Rutgeerts, Paul | - |
item.contributor | Ferrante, Marc | - |
item.contributor | Vermeire, Severine | - |
item.contributor | ARIJS, Ingrid | - |
item.contributor | Cleynen, Isabelle | - |
item.fullcitation | Verstockt, Sare; De Hertogh, Gert; Van der Goten, Jan; Verstockt, Bram; Vancamelbeke, Maaike; Machiels, Kathleen; Van Lommel, Leentje; Schuit, Frans; Van Assche, Gert; Rutgeerts, Paul; Ferrante, Marc; Vermeire, Severine; ARIJS, Ingrid & Cleynen, Isabelle (2019) Gene and Mirna Regulatory Networks During Different Stages of Crohn's Disease. In: JOURNAL OF CROHNS & COLITIS, 13(7), p. 916-930. | - |
crisitem.journal.issn | 1873-9946 | - |
crisitem.journal.eissn | 1876-4479 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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jjz007.pdf | Published version | 4.21 MB | Adobe PDF | View/Open |
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