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Title: | Activation of Eosinophils and Mast Cells in Functional Dyspepsia: an Ultrastructural Evaluation | Authors: | Vanheel, Hanne Vicario, Maria BOESMANS, Werend Vanuytsel, Tim Salvo-Romero, Eloisa Tack, Jan Farre, Ricard |
Issue Date: | 2018 | Source: | SCIENTIFIC REPORTS, 8 (Art N° 5383) | Abstract: | We recently identifed mucosal mast cell and eosinophil hyperplasia in association with a duodenal impaired barrier function in functional dyspepsia (FD). We aimed to further describe the implication of these immune cells by assessing their activation state at the ultrastructural level and by evaluating the association between impaired epithelial integrity and immune activation. Duodenal biopsies were obtained from 24 FD patients and 37 healthy controls. The ultrastructure of mast cells and eosinophils was analyzed by transmission electron microscopy. Transepithelial electrical resistance and paracellular permeability were measured to evaluate epithelial barrier function. The type of degranulation in eosinophils and mast cells was piecemeal. Eosinophils displayed higher degree of degranulation in FD patients than in controls (p<0.0001). Quantifcation revealed a decreased granular density in eosinophils of FD patients (p<0.0001). The degree of degranulation in mast cells was similar in both groups. However, a more heterogeneous profle was found in the FD group (p<0.0001). No association between epithelial integrity and the number and activation state of mucosal eosinophils and mast cells was found. We demonstrated ultrastructural changes in degranulation state of eosinophils and mast cells, suggesting that eosinophil and mast cell activation play a role in the pathophysiology of FD. | Document URI: | http://hdl.handle.net/1942/29889 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-23620-y | ISI #: | 000428618900026 | Rights: | Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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