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Title: SlZRT2 Encodes a ZIP Family Zn Transporter With Dual Localization in the Ectomycorrhizal Fungus Suillus luteus
Authors: Coninx, Laura 
Smisdom, Nick 
Kohler, Annegret
Arnauts, Natascha 
Ameloot, Marcel 
Rineau, Francois 
Colpaert, Jan, V 
Ruytinx, Joske 
Issue Date: 2019
Abstract: Ectomycorrhizal (ECM) fungi are important root symbionts of trees, as they can have significant effects on the nutrient status of plants. In polluted environments, particular ECM fungi can protect their host tree from Zn toxicity by restricting the transfer of Zn while securing supply of essential nutrients. However, mechanisms and regulation of cellular Zn homeostasis in ECM fungi are largely unknown, and it remains unclear how ECM fungi affect the Zn status of their host plants. This study focuses on the characterization of a ZIP (Zrt/IrtT-like protein) transporter, SlZRT2, in the ECM fungus Suillus luteus, a common root symbiont of young pine trees. SlZRT2 is predicted to encode a plasma membrane-located Zn importer. Heterologous expression of SlZRT2 in yeast mutants with impaired Zn uptake resulted in a minor impact on cellular Zn accumulation and growth. The SlZRT2 gene product showed a dual localization and was detected at the plasma membrane and perinuclear region. S. luteus ZIP-family Zn uptake transporters did not show the potential to induce trehalase activity in yeast and to function as Zn sensors. In response to excess environmental Zn, gene expression analysis demonstrated a rapid but minor and transient decrease in SlZRT2 transcript level. In ECM root tips, the gene is upregulated. Whether this regulation is due to limited Zn availability at the fungal-plant interface or to developmental processes is unclear. Altogether, our results suggest a function for SlZRT2 in cellular Zn redistribution from the ER next to a putative role in Zn uptake in S. luteus.
Notes: [Coninx, Laura; Arnauts, Natascha; Rineau, Francois; Colpaert, Jan, V; Ruytinx, Joske] Hasselt Univ, Environm Biol, Ctr Environm Sci, Diepenbeek, Belgium. [Smisdom, Nick; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Kohler, Annegret] Univ Lorraine Interact Arbres Microoroanismes, UMR 1136 INRA, INRA, Lab Excellence ARBRE, Champenoux, France. [Ruytinx, Joske] Vrije Univ Brussel, Res Grp Microbiol, Dept Bioengn Sci, Brussels, Belgium.
Keywords: Mycorrhiza; Suillus luteus; zinc transporter; zinc homeostasis; ZIP;Mycorrhiza; Suillus luteus; zinc transporter; zinc homeostasis; ZIP
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ISSN: 1664-302X
e-ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.02251
ISI #: 000491340600001
Rights: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Category: A1
Type: Journal Contribution
Validations: ecoom 2020
Appears in Collections:Research publications

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