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Title: | Genomic Regions Associated with IgE Levels against Culicoides spp. Antigens in Three Horse Breeds | Authors: | Francois, Liesbeth Hoskens, Hanne Velie, Brandon D. Stinckens, Anneleen Tinel, Susanne Lamberigts, Chris PEETERS, Liesbet Savelkoul, Huub E. J. Tijhaar, Edwin Lindgren, Gabriella Janssens, Steven Ducro, Bart J. Buys, Nadine Schurink, Anouk |
Issue Date: | 2019 | Publisher: | MDPI | Source: | GENES, 10(8) (Art N° 597) | Abstract: | Insect bite hypersensitivity (IBH), which is a cutaneous allergic reaction to antigens from Culicoides spp., is the most prevalent skin disorder in horses. Misdiagnosis is possible, as IBH is usually diagnosed based on clinical signs. Our study is the first to employ IgE levels against several recombinant Culicoides spp. allergens as an objective, independent, and quantitative phenotype to improve the power to detect genetic variants that underlie IBH. Genotypes of 200 Shetland ponies, 127 Icelandic horses, and 223 Belgian Warmblood horses were analyzed while using a mixed model approach. No single-nucleotide polymorphism (SNP) passed the Bonferroni corrected significance threshold, but several regions were identified within and across breeds, which confirmed previously identified regions of interest and, in addition, identifying new regions of interest. Allergen-specific IgE levels are a continuous and objective phenotype that allow for more powerful analyses when compared to a case-control set-up, as more significant associations were obtained. However, the use of a higher density array seems necessary to fully employ the use of IgE levels as a phenotype. While these results still require validation in a large independent dataset, the use of allergen-specific IgE levels showed value as an objective and continuous phenotype that can deepen our understanding of the biology underlying IBH. | Notes: | [Francois, Liesbeth; Stinckens, Anneleen; Tinel, Susanne; Lindgren, Gabriella; Janssens, Steven; Buys, Nadine] Katholieke Univ Leuven, Dept Biosyst, Livestock Genet, B-3001 Leuven, Belgium. [Hoskens, Hanne] Katholieke Univ Leuven, Dept Human Genet, B-3000 Leuven, Belgium. [Velie, Brandon D.] B19 603 Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia. [Lamberigts, Chris] Katholieke Univ Leuven, Dept Biosyst, Res Grp Livestock Physiol, B-3001 Leuven, Belgium. [Peeters, Liesbet] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Savelkoul, Huub E. J.; Tijhaar, Edwin] Wageningen Univ & Res, Cell Biol & Immunol Grp, NL-6700 AH Wageningen, Netherlands. [Lindgren, Gabriella] Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden. [Ducro, Bart J.; Schurink, Anouk] Wageningen Univ & Res, Anim Breeding & Genom, NL-6700 AH Wageningen, Netherlands. [Schurink, Anouk] Wageningen Univ & Res, Ctr Genet Resources Netherlands CGN, NL-6700 AH Wageningen, Netherlands. | Keywords: | Belgian Warmblood horse;diagnostic ELISA test;genome-wide association study;Icelandic horse;IgE;insect bite hypersensitivity;Shetland pony;summer eczema | Document URI: | http://hdl.handle.net/1942/30063 | e-ISSN: | 2073-4425 | DOI: | 10.3390/genes10080597 | ISI #: | 000483744500006 | Rights: | 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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genes-10-00597-v2.pdf | Published version | 3.1 MB | Adobe PDF | View/Open |
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