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http://hdl.handle.net/1942/30099
Title: | A Quest of Great Importance-Developing a Broad Spectrum Escherichia coli Phage Collection | Authors: | Kaczorowska, Joanna Casey, Eoghan Neve, Horst Franz, Charles M. A. P. NOBEN, Jean-Paul Lugli, Gabriele A. Ventura, Marco van Sinderen, Douwe Mahony, Jennifer |
Issue Date: | 2019 | Publisher: | MDPI | Source: | Viruses, 11 (10) (Art N° 899) | Abstract: | Shigella ssp. and enterotoxigenic Escherichia coli are the most common etiological agents of diarrheal diseases in malnourished children under five years of age in developing countries. The ever-growing issue of antibiotic resistance and the potential negative impact of antibiotic use on infant commensal microbiota are significant challenges to current therapeutic approaches. Bacteriophages (or phages) represent an alternative treatment that can be used to treat specific bacterial infections. In the present study, we screened water samples from both environmental and industrial sources for phages capable of infecting E. coli laboratory strains within our collection. Nineteen phages were isolatedand tested for their ability to infect strains within the ECOR collection and E. coli O157:H7 Delta stx. Furthermore, since coliphages have been reported to cross-infect certain Shigella spp., we also evaluated the ability of the nineteen phages to infect a representative Shigella sonnei strain from our collection. Based on having distinct (although overlapping in some cases) host ranges, ten phage isolates were selected for genome sequence and morphological characterization. Together, these ten selected phages were shown to infect most of the ECOR library, with 61 of the 72 strains infected by at least one phage from our collection. Genome analysis of the ten phages allowed classification into five previously described genetic subgroups plus one previously underrepresented subgroup. | Keywords: | bacteriophage;Escherichia coli;host range;phage therapy;Shigella ssp | Document URI: | http://hdl.handle.net/1942/30099 | e-ISSN: | 1999-4915 | DOI: | 10.3390/v11100899 | ISI #: | 000498400400020 | Rights: | 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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viruses-11-00899.pdf | Published version | 7.04 MB | Adobe PDF | View/Open |
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