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http://hdl.handle.net/1942/30335
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DC Field | Value | Language |
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dc.contributor.author | Stanga, Serena | - |
dc.contributor.author | Zanou, Nadège | - |
dc.contributor.author | Audouard, Emilie | - |
dc.contributor.author | Tasiaux, Bernadette | - |
dc.contributor.author | Contino, Sabrina | - |
dc.contributor.author | Vandermeulen, Gaëlle | - |
dc.contributor.author | René, Frédérique | - |
dc.contributor.author | Loeffler, Jean-Philippe | - |
dc.contributor.author | Clotman, Frédéric | - |
dc.contributor.author | Gailly, Philippe | - |
dc.contributor.author | DEWACHTER, Ilse | - |
dc.contributor.author | Octave, Jean-Noël | - |
dc.contributor.author | Kienlen-Campard, Pascal | - |
dc.date.accessioned | 2020-01-17T08:53:59Z | - |
dc.date.available | 2020-01-17T08:53:59Z | - |
dc.date.issued | 2016 | - |
dc.date.submitted | 2020-01-09T10:50:14Z | - |
dc.identifier.citation | FASEB JOURNAL, 30 (5) , p. 1696 -1711 | - |
dc.identifier.uri | http://hdl.handle.net/1942/30335 | - |
dc.description.abstract | Besides its crucial role in the pathogenesis of Alzheimer's disease, the knowledge of amyloid precursor protein (APP) physiologic functions remains surprisingly scarce. Here, we show that APP regulates the transcription of the glial cell line-derived neurotrophic factor (GDNF). APP-dependent regulation of GDNF expression affects muscle strength, muscular trophy, and both neuronal and muscular differentiation fundamental for neuromuscular junction (NMJ) maturation in vivo In a nerve-muscle coculture model set up to modelize NMJ formation in vitro, silencing of muscular APP induces a 30% decrease in secreted GDNF levels and a 40% decrease in the total number of NMJs together with a significant reduction in the density of acetylcholine vesicles at the presynaptic site and in neuronal maturation. These defects are rescued by GDNF expression in muscle cells in the conditions where muscular APP has been previously silenced. Expression of GDNF in muscles of amyloid precursor protein null mice corrected the aberrant synaptic morphology of NMJs. Our findings highlight for the first time that APP-dependent GDNF expression drives the process of NMJ formation, providing new insights into the link between APP gene regulatory network and physiologic functions.-Stanga, S., Zanou, N., Audouard, E., Tasiaux, B., Contino, S., Vandermeulen, G., René, F., Loeffler, J.-P., Clotman, F., Gailly, P., Dewachter, I., Octave, J.-N., Kienlen-Campard, P. APP-dependent glial cell line-derived neurotrophic factor gene expression drives neuromuscular junction formation. | - |
dc.description.sponsorship | The authors are grateful to Bart de Strooper (Katholieke Universiteit Leuven, Leuven, Belgium) for the kind gift of mouse embryonic fibroblast cells. The authors kindly acknowledge Laetitia El Haylani (Alzheimer Research Group, Universite´ Catholique de Louvain, Institute of Neuroscience, Brussels, Belgium) for her skillful technical assistance. This work was supported by a grant from the Belgian Fonds National pour la Recherche Scientifique (to S.S.), by the S.A.O./F.R.A. Stichting voor Alzheimer Onderzoek/Foundation for Research on Alzheimer’s disease (to P.K.-C.), Interuniversity Attraction Pole Programme-Belgian Sate-Belgian Science Policy (IAP-P7/16 and IAP-P7/13 to J.-N.O., P.K.-C., and P.G.), Fondation Medicale Reine Elisabeth (to J.-N.O., I.D., and P.K.-C.), and ´ Action de Recherche Concert´ee (14/19-059 to P.K.-C.). F.C. is a research associate, and N.Z. is a postdoctoral researcher of the Belgian Fonds National pour la Recherche Scientifique. | - |
dc.language.iso | en | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.rights | FASEB | - |
dc.subject.other | APP knockout mice | - |
dc.subject.other | Alzheimer’s disease | - |
dc.subject.other | gene transcription | - |
dc.subject.other | muscle electroporation | - |
dc.subject.other | nerve–muscle coculture | - |
dc.subject.other | Amyloid beta-Protein Precursor | - |
dc.subject.other | Animals | - |
dc.subject.other | Cells, Cultured | - |
dc.subject.other | Fibroblasts | - |
dc.subject.other | Gene Expression Regulation | - |
dc.subject.other | Glial Cell Line-Derived Neurotrophic Factor | - |
dc.subject.other | Mice | - |
dc.subject.other | Mice, Knockout | - |
dc.subject.other | Muscle, Skeletal | - |
dc.subject.other | Neuromuscular Junction | - |
dc.title | APP-dependent glial cell line-derived neurotrophic factor gene expression drives neuromuscular junction formation | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1711 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1696 | - |
dc.identifier.volume | 30 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.source.type | Article | - |
dc.identifier.doi | 10.1096/fj.15-278739 | - |
dc.identifier.pmid | 26718890 | - |
dc.identifier.isi | 000374879400002 | - |
dc.identifier.eissn | 1530-6860 | - |
local.provider.type | PubMed | - |
local.uhasselt.uhpub | no | - |
item.fullcitation | Stanga, Serena; Zanou, Nadège; Audouard, Emilie; Tasiaux, Bernadette; Contino, Sabrina; Vandermeulen, Gaëlle; René, Frédérique; Loeffler, Jean-Philippe; Clotman, Frédéric; Gailly, Philippe; DEWACHTER, Ilse; Octave, Jean-Noël & Kienlen-Campard, Pascal (2016) APP-dependent glial cell line-derived neurotrophic factor gene expression drives neuromuscular junction formation. In: FASEB JOURNAL, 30 (5) , p. 1696 -1711. | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.contributor | Stanga, Serena | - |
item.contributor | Zanou, Nadège | - |
item.contributor | Audouard, Emilie | - |
item.contributor | Tasiaux, Bernadette | - |
item.contributor | Contino, Sabrina | - |
item.contributor | Vandermeulen, Gaëlle | - |
item.contributor | René, Frédérique | - |
item.contributor | Loeffler, Jean-Philippe | - |
item.contributor | Clotman, Frédéric | - |
item.contributor | Gailly, Philippe | - |
item.contributor | DEWACHTER, Ilse | - |
item.contributor | Octave, Jean-Noël | - |
item.contributor | Kienlen-Campard, Pascal | - |
crisitem.journal.issn | 0892-6638 | - |
crisitem.journal.eissn | 1530-6860 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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fj.15-278739.pdf | Published version | 2.43 MB | Adobe PDF | View/Open |
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