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http://hdl.handle.net/1942/30339
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DC Field | Value | Language |
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dc.contributor.author | Hage, Salim | - |
dc.contributor.author | Stanga, Serena | - |
dc.contributor.author | Marinangeli, Claudia | - |
dc.contributor.author | Octave, Jean-Noël | - |
dc.contributor.author | DEWACHTER, Ilse | - |
dc.contributor.author | Quetin-Leclercq, Joëlle | - |
dc.contributor.author | Kienlen-Campard, Pascal | - |
dc.date.accessioned | 2020-01-17T11:41:20Z | - |
dc.date.available | 2020-01-17T11:41:20Z | - |
dc.date.issued | 2015 | - |
dc.date.submitted | 2020-01-09T11:30:19Z | - |
dc.identifier.citation | JOURNAL OF ETHNOPHARMACOLOGY, 163 , p. 192-202 -202 | - |
dc.identifier.uri | http://hdl.handle.net/1942/30339 | - |
dc.description.abstract | The aqueous decoction of Pterocarpus erinaceus has been traditionally used in Benin against memory troubles. | - |
dc.description.abstract | Ethnopharmacological relevance: The aqueous decoction of Pterocarpus erinaceus has been traditionally used in Benin against memory troubles.Aim of the study: New strategies are needed against Alzheimer's disease (AD), for, to date, AD treatment is symptomatic and consists in drugs treating the cognitive decline. An interesting target is the beta-amyloid peptide (A beta), whose accumulation and progressive deposition into amyloid plaques are key events in AD aetiology.Identifying new and more selective gamma-secretase inhibitors or modulators (none of the existing has proven so far to be selective or fully efficient) appears in this respect of particular interest. We studied the activity and mechanisms of action of Pterocarpus erinaceus kino aqueous extract, after the removal of catechic tannins (KAST).Methods and results: We tested KAST at non-toxic concentrations on cells expressing the human Amyloid Precursor Protein (APP695), as well as on primary neurons. Pterocarpus erinaceus extract was found to inhibit A beta release in both models. We further showed that MAST inhibited gamma-secretase activity in cell-free and in vitro assays, strongly suggesting that MAST is a natural gamma-secretase inhibitor. Importantly, this extract did not inhibit the cleavage of Notch, another gamma-secretase substrate responsible for major detrimental side effects observed with gamma-secretase inhibitors. Epicatechin was further identified in MAST by HPLC-MS.Conclusion: Pteroanpus erinaceus kino extract appears therefore as a new gamma-secretase inhibitor selective towards APP processing. (C) 2015 Elsevier Ireland Ltd. All rights reserved. | - |
dc.description.sponsorship | This work was supported by a grant of the Belgian F.R.I.A. (Fondspour la Formation à la Recherche dans l’Industrie et l’Agriculture) foll-owed by a grant of theCommission du Patrimoineof UCL (SH), and bytheS.A.O./F.R.A.Foundation for Research on Alzheimer's Disease-King Baudoin Foundation (PKC), as well as by the InteruniversityAttraction Pole Programme-Belgian Sate-Belgian Science Policy (P7/16) (JNO, ID and PKC). We also thank the Belgian National Fund forScientific Research (FNRS) (FRFC 2.4555.08) and the Special Fund forResearch (FSR) for their support. Didier Lambert (LDRI,U.C.L.)isgratefully acknowledged for the kind gift of CHO cells (ATCC, CCL-61), and for the use of technical facilities. We are also grateful to PierreAgbani and Fernand Gbaguidi (Abomey-Calavi University, Benin) forplant collection and identification, to Bart de Strooper (KU Leuven)forthe kind gift of pHES2-luciferase plasmid and of MEF cells, to Marie-France Hérent (LDRI,U.C.L.) for HPLC-MS analysis, to Giulio Muccioli(LDRI,U.C.L.) and Céline Rivière (Université de Lille 2) for usefuldiscussions, to Marie-Christine Fayt, Bernadette Tasiaux and LaetitiaEl Haylani for their skilful technical assistance, to Laurence Timmer-mans for her contribution to the luciferase assays. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.rights | 2015 Elsevier Ireland Ltd. All rights reserved. | - |
dc.subject.other | Alzheimer׳s disease | - |
dc.subject.other | Kino | - |
dc.subject.other | Notch cleavage | - |
dc.subject.other | Pterocarpus erinaceus | - |
dc.subject.other | β-amyloid peptide | - |
dc.subject.other | γ-secretase inhibitor | - |
dc.subject.other | Amyloid Precursor Protein Secretases | - |
dc.subject.other | Amyloid beta-Peptides | - |
dc.subject.other | Animals | - |
dc.subject.other | CHO Cells | - |
dc.subject.other | Cell Survival | - |
dc.subject.other | Cells, Cultured | - |
dc.subject.other | Cricetinae | - |
dc.subject.other | Cricetulus | - |
dc.subject.other | Mice | - |
dc.subject.other | Neurons | - |
dc.subject.other | Plant Bark | - |
dc.subject.other | Plant Extracts | - |
dc.subject.other | Plant Gums | - |
dc.subject.other | Pterocarpus | - |
dc.title | Characterization of Pterocarpus erinaceus kino extract and its gamma-secretase inhibitory properties | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 202 | - |
dc.identifier.spage | 192-202 | - |
dc.identifier.volume | 163 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.source.type | Article | - |
dc.identifier.doi | 10.1016/j.jep.2015.01.028 | - |
dc.identifier.pmid | 25639816 | - |
dc.identifier.isi | 000351797300020 | - |
dc.identifier.eissn | 1872-7573 | - |
local.provider.type | PubMed | - |
local.uhasselt.uhpub | no | - |
item.fullcitation | Hage, Salim; Stanga, Serena; Marinangeli, Claudia; Octave, Jean-Noël; DEWACHTER, Ilse; Quetin-Leclercq, Joëlle & Kienlen-Campard, Pascal (2015) Characterization of Pterocarpus erinaceus kino extract and its gamma-secretase inhibitory properties. In: JOURNAL OF ETHNOPHARMACOLOGY, 163 , p. 192-202 -202. | - |
item.fulltext | With Fulltext | - |
item.accessRights | Restricted Access | - |
item.contributor | Hage, Salim | - |
item.contributor | Stanga, Serena | - |
item.contributor | Marinangeli, Claudia | - |
item.contributor | Octave, Jean-Noël | - |
item.contributor | DEWACHTER, Ilse | - |
item.contributor | Quetin-Leclercq, Joëlle | - |
item.contributor | Kienlen-Campard, Pascal | - |
crisitem.journal.issn | 0378-8741 | - |
crisitem.journal.eissn | 1872-7573 | - |
Appears in Collections: | Research publications |
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1-s2.0-S0378874115000434-main.pdf Restricted Access | Published version | 1.19 MB | Adobe PDF | View/Open Request a copy |
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