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http://hdl.handle.net/1942/30342
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DC Field | Value | Language |
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dc.contributor.author | STANCU, Ilie Cosmin | - |
dc.contributor.author | Couturier, Julien | - |
dc.contributor.author | Schakman, Olivier | - |
dc.contributor.author | Pierrot, Nathalie | - |
dc.contributor.author | Huaux, François | - |
dc.contributor.author | Kienlen-Campard, Pascal | - |
dc.contributor.author | DEWACHTER, Ilse | - |
dc.contributor.author | Octave, Jean-Noël | - |
dc.date.accessioned | 2020-01-17T12:28:45Z | - |
dc.date.available | 2020-01-17T12:28:45Z | - |
dc.date.issued | 2016 | - |
dc.date.submitted | 2020-01-09T10:26:15Z | - |
dc.identifier.citation | Journal of neuroinflammation, 13 (1) (Art N° ARTN 20) | - |
dc.identifier.uri | http://hdl.handle.net/1942/30342 | - |
dc.description.abstract | The proinflammatory cytokine interleukin-1β (IL-1β) is overexpressed in Alzheimer disease (AD) as a key regulator of neuroinflammation. Amyloid-β (Aβ) peptide triggers activation of inflammasomes, protein complexes responsible for IL-1β maturation in microglial cells. Downregulation of NALP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome has been shown to decrease amyloid load and rescue cognitive deficits in a mouse model of AD. Whereas activation of inflammasome in microglial cells has been described in AD, no data are currently available concerning activation of inflammasome in astrocytes, although they are involved in inflammatory response and phagocytosis. Here, by targeting the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD domain), we investigated the influence of activation of the inflammasome on the phagocytic activity of astrocytes. | - |
dc.description.abstract | Background: The proinflammatory cytokine interleukin-1 beta (IL-1 beta) is overexpressed in Alzheimer disease (AD) as a key regulator of neuroinflammation. Amyloid-beta (A beta) peptide triggers activation of inflammasomes, protein complexes responsible for IL-1 beta maturation in microglial cells. Downregulation of NALP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome has been shown to decrease amyloid load and rescue cognitive deficits in a mouse model of AD. Whereas activation of inflammasome in microglial cells has been described in AD, no data are currently available concerning activation of inflammasome in astrocytes, although they are involved in inflammatory response and phagocytosis. Here, by targeting the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD domain), we investigated the influence of activation of the inflammasome on the phagocytic activity of astrocytes.Methods: We used an ASC knockout mouse model, as ASC is a central protein in the inflammasome, acting as an adaptor and stabilizer of the complex and thus critical for its activation. Lipopolysaccharide (LPS)-primed primary cultures of astrocytes from newborn mice were utilized to evaluate A beta-induced inflammasome activation by measuring IL-1 beta release by ECLIA (electro-chemiluminescence immunoassay). Phagocytosis efficiency was measured by incorporation of bioparticles, and the release of the chemokine CCL3 (C-C motif ligand 3) was measured by ECLIA. ASC mice were crossbred with 5xFAD (familial Alzheimer disease) mice and tested for spatial reference memory using the Morris water maze (MWM) at 7-8 months of age. Amyloid load and CCL3 were quantified by thioflavine S staining and quantitative real-time polymerase chain reaction (qRT-PCR), respectively.Results: Cultured astrocytes primed with LPS and treated with A beta showed an ASC-dependent production of IL-1 beta resulting from inflammasome activation mediated by A beta phagocytosis and cathepsin B enzymatic activity. ASC+/- astrocytes displayed a higher phagocytic activity as compared to ASC+/+ and ASC -/- cells, resulting from a higher release of the chemokine CCL3. A significant decrease in amyloid load was measured in the brain of 7-8-month-old 5xFAD mice carrying the ASC +/- genotype, correlated with an increase in CCL3 gene expression. In addition, the ASC +/- genotype rescued spatial reference memory deficits observed in 5xFAD mice.Conclusions: Our results demonstrate that A beta is able to activate astrocytic inflammasome. Downregulation of inflammasome activity increases phagocytosis in astrocytes due to the release of CCL3. This could explain why downregulation of inflammasome activity decreases amyloid load and rescues memory deficits in a mouse model of AD. | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject.other | Alzheimer disease | - |
dc.subject.other | Inflammasome | - |
dc.subject.other | ASC | - |
dc.subject.other | Astrocytes | - |
dc.subject.other | CCL3 | - |
dc.subject.other | amyloid-beta | - |
dc.subject.other | Phagocytosis | - |
dc.subject.other | Alzheimer Disease | - |
dc.subject.other | Amyloid beta-Peptides | - |
dc.subject.other | Amyloid beta-Protein Precursor | - |
dc.subject.other | Animals | - |
dc.subject.other | Animals, Newborn | - |
dc.subject.other | Apoptosis Regulatory Proteins | - |
dc.subject.other | CARD Signaling Adaptor Proteins | - |
dc.subject.other | Case-Control Studies | - |
dc.subject.other | Cells, Cultured | - |
dc.subject.other | Chemokine CCL3 | - |
dc.subject.other | Cytokines | - |
dc.subject.other | Disease Models, Animal | - |
dc.subject.other | Gene Expression Regulation | - |
dc.subject.other | Humans | - |
dc.subject.other | Ionophores | - |
dc.subject.other | Memory Disorders | - |
dc.subject.other | Mice | - |
dc.subject.other | Mice, Inbred C57BL | - |
dc.subject.other | Mice, Transgenic | - |
dc.subject.other | Mutation | - |
dc.subject.other | Nigericin | - |
dc.subject.other | Peptide Fragments | - |
dc.subject.other | Phagocytes | - |
dc.subject.other | Presenilin-1 | - |
dc.title | Activation of phagocytic activity in astrocytes by reduced expression of the inflammasome component ASC and its implication in a mouse model of Alzheimer disease | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 1 | - |
dc.identifier.volume | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | ARTN 20 | - |
local.class | IncludeIn-ExcludeFrom-List/ExcludeFromFRIS | - |
dc.source.type | Article | - |
dc.identifier.doi | 10.1186/s12974-016-0477-y | - |
dc.identifier.pmid | 26818951 | - |
dc.identifier.isi | 000368886900001 | - |
local.provider.type | PubMed | - |
local.uhasselt.uhpub | no | - |
item.accessRights | Closed Access | - |
item.contributor | STANCU, Ilie Cosmin | - |
item.contributor | Couturier, Julien | - |
item.contributor | Schakman, Olivier | - |
item.contributor | Pierrot, Nathalie | - |
item.contributor | Huaux, François | - |
item.contributor | Kienlen-Campard, Pascal | - |
item.contributor | DEWACHTER, Ilse | - |
item.contributor | Octave, Jean-Noël | - |
item.fullcitation | STANCU, Ilie Cosmin; Couturier, Julien; Schakman, Olivier; Pierrot, Nathalie; Huaux, François; Kienlen-Campard, Pascal; DEWACHTER, Ilse & Octave, Jean-Noël (2016) Activation of phagocytic activity in astrocytes by reduced expression of the inflammasome component ASC and its implication in a mouse model of Alzheimer disease. In: Journal of neuroinflammation, 13 (1) (Art N° ARTN 20). | - |
item.fulltext | No Fulltext | - |
crisitem.journal.eissn | 1742-2094 | - |
Appears in Collections: | Research publications |
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