Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/30674
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dc.contributor.authorVREYS, Frederik-
dc.contributor.authorSLAETS, Leen-
dc.contributor.authorSOMERS, Veerle-
dc.contributor.authorTHOELEN, Ronald-
dc.date.accessioned2020-03-05T13:58:15Z-
dc.date.available2020-03-05T13:58:15Z-
dc.date.issued2019-
dc.date.submitted2020-03-03T16:06:20Z-
dc.identifier.citationthe EnFI 2019 conference., Leuven, Belgium, 8-9 juli 2019-
dc.identifier.urihttp://hdl.handle.net/1942/30674-
dc.description.abstractA high throughput solid-phase enzyme immunoassay(EIA) platform based on lab-on-card(LOC) microfluidics is proposed. In addition, a first step towards label-free detection of immunosorbent assays based on Electrical Impedance Spectroscopy(EIS) is made. The reaction zones in the LOC contain aluminium interdigitated electrodes which are coated with polystyrene. The binding capacity of the coating was tested by measuring the contact angle. Furthermore, the end product of a colorimetric ELISA test was flushed into the LOC and the optical density was measured. The preliminary tests provide a stable base for the label-free EIA based on EIS.-
dc.description.sponsorshipThis work is funded by the HEALTHY AGING project which is carried out under Interreg V-A Euregio Meuse - Rhine and is supported by the European Union and The European Regional Development Fund and with financial support of province of Limburg - Belgium.-
dc.language.isoen-
dc.publisherEngineering Functional Interfaces (EnFI)-
dc.subject.othermicrofluidics-
dc.subject.otherlab-on-card(LOC)-
dc.subject.otherlabel-free-
dc.subject.otherenzyme immunoassay (EIA)-
dc.subject.otherElectrical Impedance Spectroscopy(EIS)-
dc.subject.otherSPAG-16-
dc.titleTowards lab-on-card, label-free immunoassays based on electrical impedance spectroscopy-
dc.typeConference Material-
local.bibliographicCitation.conferencedate8-9 juli 2019-
local.bibliographicCitation.conferencenamethe EnFI 2019 conference.-
local.bibliographicCitation.conferenceplaceLeuven, Belgium-
local.format.pages4-
local.bibliographicCitation.jcatC2-
dc.relation.references[1] T.-C. Tseng, T. et al., J. Electroanal. Chem. 2015, doi: 10.1016/j.jelechem.2015.10.017 [2] de Bock, L. et al., J Immunol 2014, doi: 10.4049/jimmunol.1401166-
local.type.refereedNon-Refereed-
local.type.specifiedConference Presentation-
local.uhasselt.uhpubyes-
item.accessRightsRestricted Access-
item.fulltextWith Fulltext-
item.fullcitationVREYS, Frederik; SLAETS, Leen; SOMERS, Veerle & THOELEN, Ronald (2019) Towards lab-on-card, label-free immunoassays based on electrical impedance spectroscopy. In: the EnFI 2019 conference., Leuven, Belgium, 8-9 juli 2019.-
item.contributorVREYS, Frederik-
item.contributorSLAETS, Leen-
item.contributorSOMERS, Veerle-
item.contributorTHOELEN, Ronald-
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