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http://hdl.handle.net/1942/30790
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DC Field | Value | Language |
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dc.contributor.author | Sharma, H.S. | - |
dc.contributor.author | Alagappan, V.K.T. | - |
dc.contributor.author | WIDYASTUTI, Anna | - |
dc.contributor.author | Mooi, W.J. | - |
dc.contributor.author | de Boer, W.I. | - |
dc.date.accessioned | 2020-03-13T11:30:02Z | - |
dc.date.available | 2020-03-13T11:30:02Z | - |
dc.date.issued | 2013 | - |
dc.date.submitted | 2020-03-13T07:49:52Z | - |
dc.identifier.citation | Cell Biochemistry and Biophysics, Cell Biochemistry and Biophysics, 67 (2) , p. 247 -254 | - |
dc.identifier.uri | http://hdl.handle.net/1942/30790 | - |
dc.description.abstract | Angiogenesis and microvascular leakage are features of chronic inflammatory diseases of which molecular mechanisms are poorly understood. We investigated the effects of interleukin-1 beta (IL-1 beta) on the expression and secretion of vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) in porcine airway smooth muscle cells (PASMC) in relation to a nitric oxide (NO) pathway. Serum-deprived (48 h) PASMC were stimulated with IL-1 beta alone or with NO donor, l-arginine and/or NO synthase inhibitor l-NAME for 4 and 24 h. IL-1 beta did not affect PlGF release, but augmented VEGF release (2.4-fold) after 24 h. VEGF release was inhibited by l-NAME (531.8 +/- A 52 pg/ml), but restored and further elevated by l-arginine (1,529 +/- A 287 pg/ml). IL-1 beta up-regulated VEGF mRNA (1.8-fold) and this response was attenuated by l-NAME (1.1-fold) and augmented by l-arginine (3.8-fold) at 4 h. Restoration of a NO pathway by l-arginine in l-NAME-treated cells resulted in elevated VEGF mRNA levels (2.2-fold). [H-3]Thymidine incorporation assay revealed enhanced porcine pulmonary artery endothelial cell proliferation in response to IL-1 beta, VEGF and PlGF, and this mitogenic effect was not influenced via the NO pathway. Our results suggest that a NO pathway modulates VEGF synthesis during inflammation contributing to bronchial angiogenesis and vascular leakage. | - |
dc.description.sponsorship | This study was supported in part by a research Grant to HSS at his Cardiopulmonary Molecular Biology lab, Erasmus MC, Rotterdam from the Netherlands Asthma Foundation. | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.publisher | - | |
dc.rights | Springer Science+Business Media New York 2013 | - |
dc.subject.other | Airway smooth muscle | - |
dc.subject.other | Angiogenesis | - |
dc.subject.other | VEGF | - |
dc.subject.other | Nitric oxide | - |
dc.subject.other | Interleukin-1 beta | - |
dc.title | Nitric Oxide Donors Augment Interleukin-1β-Induced Vascular Endothelial Growth Factor in Airway Smooth Muscle Cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 254 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 247 | - |
dc.identifier.volume | 67 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.source.type | journal-article | - |
dc.identifier.doi | 10.1007/s12013-013-9773-7 | - |
dc.identifier.pmid | 24142467 | - |
dc.identifier.scopus | 2-s2.0-84886584460 | - |
dc.identifier.isi | WOS:000326282900004 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?eid=2-s2.0-84886584460&partnerID=MN8TOARS | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.identifier.eissn | 1559-0283 | - |
local.provider.type | Orcid | - |
local.uhasselt.uhpub | no | - |
item.contributor | Sharma, H.S. | - |
item.contributor | Alagappan, V.K.T. | - |
item.contributor | WIDYASTUTI, Anna | - |
item.contributor | Mooi, W.J. | - |
item.contributor | de Boer, W.I. | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Sharma, H.S.; Alagappan, V.K.T.; WIDYASTUTI, Anna; Mooi, W.J. & de Boer, W.I. (2013) Nitric Oxide Donors Augment Interleukin-1β-Induced Vascular Endothelial Growth Factor in Airway Smooth Muscle Cells. In: Cell Biochemistry and Biophysics, Cell Biochemistry and Biophysics, 67 (2) , p. 247 -254. | - |
item.fulltext | With Fulltext | - |
Appears in Collections: | Research publications |
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Sharma2013_Article_NitricOxideDonorsAugmentInterl.pdf Restricted Access | Published version | 356.02 kB | Adobe PDF | View/Open Request a copy |
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