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http://hdl.handle.net/1942/3088
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DC Field | Value | Language |
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dc.contributor.author | Petzel, DH | - |
dc.contributor.author | PIROTTE, Patrick | - |
dc.contributor.author | VAN KERKHOVE, Emmy | - |
dc.date.accessioned | 2007-11-23T15:24:59Z | - |
dc.date.available | 2007-11-23T15:24:59Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | JOURNAL OF INSECT PHYSIOLOGY, 45(11). p. 973-982 | - |
dc.identifier.issn | 0022-1910 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3088 | - |
dc.description.abstract | In order to understand the critical role that hydrogen ions play in fluid secretion in Malpighian tubules, intracellular and luminal pH and K+ measurements were performed in isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti). The intracellular pH was 7.03+/-0.05 (n=15 Malpighian tubules (MT)) and the luminal pH was 7.19+/-0.09 (n=99 MT) when bathed in saline at a pH of 7.0, The lumen potential is positive, thus net proton secretion into the lumen is active. The intracellular and the luminal K+ concentrations were 75+/-9 mM (n=15) and 102+/-13 mM (n=9 MT) respectively. Cyclic AMP analogues accelerated fluid secretion and at the same time acidified the cell without affecting the luminal pH. Both effects were abolished by an isomer of adenosine-3',5' cyclic monophosphothioate (cAMPS), the Rp-cAMPS, known to inhibit protein kinase A. The results suggest that in the presence of cAMP the properties of the cation/H+ exchanger are affected and that this may be a result of phosphorylation of a Na+/2H+ antiporter located on the apical membrane. (C) 1999 Elsevier Science Ltd. All rights reserved. | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject.other | Malpighian tubule; cyclicAMP; microfluorescence; pH; potassium | - |
dc.title | Intracellular and luminal pH measurements of Malpighian tubules of the mosquito Aedes aegypti: the effects of cAMP | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 982 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 973 | - |
dc.identifier.volume | 45 | - |
local.format.pages | 10 | - |
dc.description.notes | Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA. Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium.Petzel, DH, Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/S0022-1910(99)00076-1 | - |
dc.identifier.isi | 000082195600002 | - |
item.fulltext | No Fulltext | - |
item.fullcitation | Petzel, DH; PIROTTE, Patrick & VAN KERKHOVE, Emmy (1999) Intracellular and luminal pH measurements of Malpighian tubules of the mosquito Aedes aegypti: the effects of cAMP. In: JOURNAL OF INSECT PHYSIOLOGY, 45(11). p. 973-982. | - |
item.contributor | Petzel, DH | - |
item.contributor | PIROTTE, Patrick | - |
item.contributor | VAN KERKHOVE, Emmy | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2000 | - |
Appears in Collections: | Research publications |
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