Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/31033
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dc.contributor.authorVREYS, Frederik-
dc.contributor.authorSLAETS, Leen-
dc.contributor.authorSOMERS, Veerle-
dc.contributor.authorTHOELEN, Ronald-
dc.date.accessioned2020-04-20T07:41:52Z-
dc.date.available2020-04-20T07:41:52Z-
dc.date.issued2019-
dc.date.submitted2020-04-14T14:45:18Z-
dc.identifier.citationBelgian Physcial Society, Bruxelles, Belgium, 22 mei 2019-
dc.identifier.urihttp://hdl.handle.net/1942/31033-
dc.description.abstractA high throughput solid-phase enzyme immunoassay (EIA) platform based on lab-on-card (LOC) microfluidics is proposed. In addition, a first step towards label-free detection of immunosorbent assays based on Electrical Impedance Spectroscopy (EIS) is made. The reported LOCs are developed by alternately stacking PET layers and adhesive layers. LOCs can be equipped with aluminium interdigitated electrodes and coated with polystyrene to perform EIS. Furthermore, the end product of a colorimetric ELISA test was flushed into a LOC and subsequently the optical density was measured. The preliminary tests provide a stable base for the label-free EIA based on EIS.-
dc.description.sponsorshipThis work is funded by the HEALTHY AGING project which is carried out under Interreg V-A Euregio Meuse - Rhine and is supported by the European Union and The European Regional Development Fund and with financial support of province of Limburg - Belgium.-
dc.language.isoen-
dc.publisherBelgian Physical Society-
dc.subject.otherimmunoassay-
dc.subject.otherlab-on-card-
dc.subject.othermicrofluidics-
dc.subject.otherlabel-free-
dc.subject.otherElectrical impedance-
dc.subject.otherELISA-
dc.titleTowards lab-on-card, label-free enzyme immunoassays based on electrical impedance spectroscopy-
dc.typeConference Material-
local.bibliographicCitation.conferencedate22 mei 2019-
local.bibliographicCitation.conferencenameBelgian Physcial Society-
local.bibliographicCitation.conferenceplaceBruxelles, Belgium-
local.bibliographicCitation.jcatC2-
local.publisher.placeBelgian Physical Society 2019-
dc.relation.references[1] T.-C. Tseng, ”A simple, sensitive and compact electrochemical ELISA for estradiol based on chitosan deposited platinum wire microelectrodes” J. Electroanal. Chem. vol. 758, pp. 59-67, 2015. [2] de Bock, ”Sperm-Associated Antigen 16 Is a Novel Target of the Humoral Autoimmune Response in Multiple Sclerosis”J. Immunol. vol. 193, pp. 2147-2156, 2014.-
local.type.refereedNon-Refereed-
local.type.specifiedConference Material-
local.provider.typePdf-
local.uhasselt.uhpubyes-
item.fullcitationVREYS, Frederik; SLAETS, Leen; SOMERS, Veerle & THOELEN, Ronald (2019) Towards lab-on-card, label-free enzyme immunoassays based on electrical impedance spectroscopy. In: Belgian Physcial Society, Bruxelles, Belgium, 22 mei 2019.-
item.contributorVREYS, Frederik-
item.contributorSLAETS, Leen-
item.contributorSOMERS, Veerle-
item.contributorTHOELEN, Ronald-
item.accessRightsOpen Access-
item.fulltextWith Fulltext-
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