Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/31115
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dc.contributor.authorVREYS, Frederik-
dc.contributor.authorJAFRANE, Lamiaa-
dc.contributor.authorZAIN, A.-
dc.contributor.authorBENHASSOU, Hassan Ait-
dc.contributor.authorLakssir, B.-
dc.contributor.authorRESSAMI, El Mostafa-
dc.contributor.authorTHOELEN, Ronald-
dc.date.accessioned2020-04-24T11:47:42Z-
dc.date.available2020-04-24T11:47:42Z-
dc.date.issued2019-
dc.date.submitted2020-04-14T11:35:09Z-
dc.date.submitted2020-04-14T11:35:09Z-
dc.identifier.citationEngineering Functional Interfaces, Heverlee, Belgium, 8-9 july 2019-
dc.identifier.urihttp://hdl.handle.net/1942/31115-
dc.description.abstractThis work aims to translate lab-scale techniques for the detection of diseases towards lab-on-card(LOC) devices. Indeed, these point-of-care(POC) tests allow detecting diseases in resource-limited areas. The necessary functions of the LOC can be split up into four different sections: extraction of the cells from the biological sample, lysis of the cells to harvest DNA, DNA amplification based on loop-mediated isothermal amplification(LAMP) and DNA detection by adding SYBR Green I nucleic acid stain. These functionalities have separately been tested on LOCs. The results of the individual functions show that a fully functional POC device is an achievable goal.-
dc.description.sponsorshipFinancial support of the special research fund BOF of Hasselt University and VLIRUOS project MA2018TEA467A104.-
dc.language.isoen-
dc.publisherEngineering Functional Interfaces-
dc.subject.otherLab-on-card-
dc.subject.otherPoint-of-care-
dc.subject.otherSS-LAMP-
dc.subject.otherLAMP-
dc.subject.otherLysis-
dc.subject.otherMicrofluidics-
dc.subject.otherCell extraction-
dc.subject.otherSYBR Green I-
dc.subject.otherDNA-
dc.titleFrom lab-scale to lab-on-card: Loop-mediated isothermal amplification of biological samples-
dc.typeConference Material-
local.bibliographicCitation.conferencedate2019, July 8-9-
local.bibliographicCitation.conferencenameEngineering Functional Interfaces-
local.bibliographicCitation.conferenceplaceHeverlee, Belgium-
local.bibliographicCitation.jcatC2-
local.publisher.placeEnFI conference 2019-
dc.relation.references[1] Bentaleb, E.M. et al., BMC Infectious Diseases 2016, doi: 10.1186/s12879-016-1864-9. [2] Huang, P.-H. et al., Lab on a Chip 2015, doi: 10.1039/c5lc00539f.-
local.type.refereedNon-Refereed-
local.type.specifiedConference Poster-
local.provider.typePdf-
local.uhasselt.uhpubyes-
item.contributorVREYS, Frederik-
item.contributorJAFRANE, Lamiaa-
item.contributorZAIN, A.-
item.contributorBENHASSOU, Hassan Ait-
item.contributorLakssir, B.-
item.contributorRESSAMI, El Mostafa-
item.contributorTHOELEN, Ronald-
item.fullcitationVREYS, Frederik; JAFRANE, Lamiaa; ZAIN, A.; BENHASSOU, Hassan Ait; Lakssir, B.; RESSAMI, El Mostafa & THOELEN, Ronald (2019) From lab-scale to lab-on-card: Loop-mediated isothermal amplification of biological samples. In: Engineering Functional Interfaces, Heverlee, Belgium, 8-9 july 2019.-
item.fulltextWith Fulltext-
item.accessRightsOpen Access-
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