Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/31184
Title: Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles
Authors: MERCKX, Greet 
HOSSEINKHANI, Baharak 
KUYPERS, Soren 
DEVILLE, Sarah 
IROBI, Joy 
Nelissen, Inge
MICHIELS, Luc 
LAMBRICHTS, Ivo 
BRONCKAERS, Annelies 
Issue Date: 2020
Publisher: MDPI
Source: CELLS, 9 (2) (Art N° 312)
Abstract: Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs is associated with several drawbacks, which could be overcome by MSC-like cells of the teeth, called dental pulp stromal cells (DPSCs). This study aims to compare the angiogenic content and functions of DPSC and BM-MSC EVs and conditioned medium (CM). The angiogenic protein profile of DPSC- and BM-MSC-derived EVs, CM and EV-depleted CM was screened by an antibody array and confirmed by ELISA. Functional angiogenic effects were tested in transwell migration and chicken chorioallantoic membrane assays. All secretion fractions contained several pro- and anti-angiogenic proteins and induced in vitro endothelial cell motility. This chemotactic potential was higher for (EV-depleted) CM, compared to EVs with a stronger effect for BM-MSCs. Finally, BM-MSC CM, but not DPSC CM, nor EVs, increased in ovo angiogenesis. In conclusion, we showed that DPSCs are less potent in relation to endothelial cell chemotaxis and in ovo neovascularization, compared to BM-MSCs, which emphasizes the importance of choice of cell type and secretion fraction for stem cell-based regenerative therapies in inducing angiogenesis.
Notes: Bronckaers, A (reprint author), UHasselt Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst BIOMED, Agoralaan, B-3590 Diepenbeek, Belgium.
greet.merckx@uhasselt.be; baharak.hosseinkhani@uhasselt.be;
soren.kuypers@uhasselt.be; sarahdeville89@gmail.com;
joy.irobi@uhasselt.be; inge.nelissen@vito.be; luc.michiels@uhasselt.be;
ivo.lambrichts@uhasselt.be; annelies.bronckaers@uhasselt.be
Other: Bronckaers, A (reprint author), UHasselt Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst BIOMED, Agoralaan, B-3590 Diepenbeek, Belgium. greet.merckx@uhasselt.be; baharak.hosseinkhani@uhasselt.be; soren.kuypers@uhasselt.be; sarahdeville89@gmail.com; joy.irobi@uhasselt.be; inge.nelissen@vito.be; luc.michiels@uhasselt.be; ivo.lambrichts@uhasselt.be; annelies.bronckaers@uhasselt.be
Keywords: extracellular vesicles;bone marrow-derived mesenchymal stromal cells;dental pulp stromal cells;angiogenesis
Document URI: http://hdl.handle.net/1942/31184
e-ISSN: 2073-4409
DOI: 10.3390/cells9020312
ISI #: WOS:000521944900053
Rights: 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Category: A1
Type: Journal Contribution
Validations: ecoom 2021
Appears in Collections:Research publications

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