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http://hdl.handle.net/1942/3132
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DC Field | Value | Language |
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dc.contributor.author | ZEISKE, Wolfgang | - |
dc.contributor.author | SMETS, Ilse | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | STEELS, Paul | - |
dc.contributor.author | VAN DRIESSCHE, Willy | - |
dc.date.accessioned | 2007-11-26T15:43:45Z | - |
dc.date.available | 2007-11-26T15:43:45Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 277(3). p. C469-C479 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3132 | - |
dc.description.abstract | Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na+ transport. Am. J. Physiol. 277 (Cell Physiol. 46): C469-C479, 1999.-We report, for the epithelial Nat channel (ENaC) in A6 cells, the modulation by cell pH (pH(c)) of the transepithelial Na+ current (I-Na), the current through the individual Na+ channel (i), the open Nat channel density (N-o), and the kinetic parameters of the relationship between INa and the apical Naf concentration. The i and N-o, were evaluated from the Lorentzian IN, noise induced by the apical Na+ channel blocker 6-chloro-3,5-diaminopyrazine-2-carboxamide pH(c) shifts were induced, under strict and volume-controlled experimental conditions, by apical/basolateral NH4Cl pulses or basolateral arrest of the Na+/H+ exchanger (Nat removal; block by ethylisopropylamiloride) and were measured with the pH-sensitive probe 2',7'-bis(2-carboxyethyl)-5(6)-carboxy-fluorescein. The changes in pH, were positively correlated to changes in IN, and the apically dominated transepithelial conductance. The sole pH(c)-sensitive parameter underlying INa was N-o. Only the saturation value of the INa kinetics was subject to changes in pH(c). pH(c)-dependent changes in N-o may be caused by influencing P-o, the ENaC open probability, or/and the total channel number, N-T = N-o/P-o. | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.subject.other | noise analysis; single-channel current; epithelial sodium channel; ammonium; cell volume | - |
dc.title | Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na+ transport | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | C479 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | C469 | - |
dc.identifier.volume | 277 | - |
local.format.pages | 11 | - |
dc.description.notes | Catholic Univ Louvain, Physiol Lab, B-3000 Louvain, Belgium. Limburgs Univ Ctr, Physiol Lab, B-3590 Diepenbeek, Belgium.Van Driessche, W, Catholic Univ Louvain, Physiol Lab, Campus Gasthuisberg, B-3000 Louvain, Belgium.Willy.VanDriessche@med.KULeuven.ac.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.isi | 000082388800014 | - |
item.contributor | ZEISKE, Wolfgang | - |
item.contributor | SMETS, Ilse | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | STEELS, Paul | - |
item.contributor | VAN DRIESSCHE, Willy | - |
item.validation | ecoom 2000 | - |
item.fullcitation | ZEISKE, Wolfgang; SMETS, Ilse; AMELOOT, Marcel; STEELS, Paul & VAN DRIESSCHE, Willy (1999) Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na+ transport. In: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 277(3). p. C469-C479. | - |
item.accessRights | Closed Access | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Research publications |
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