Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/31386
Title: | Association between long-term air pollution exposure and DNA methylation: The REGICOR study | Authors: | Sayols-Baixeras, Sergi Fernandez-Sanles, Alba Prats-Uribe, Albert Subirana, Isaac PLUSQUIN, Michelle Kuenzli, Nino Marrugat, Jaume Basagana, Xavier Elosua, Roberto |
Issue Date: | 2019 | Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE | Source: | Environmental research (New York, N.Y. Print), 176 (Art N° 108550) | Abstract: | Introduction: Limited evidence suggests that epigenetic mechanisms may partially mediate the adverse effects of air pollution on health. Our aims were to identify new genomic loci showing differential DNA methylation associated with long-term exposure to air pollution and to replicate loci previously identified in other studies. Methods: A two-stage epigenome-wide association study was designed: 630 individuals from the REGICOR study were included in the discovery and 454 participants of the EPIC-Italy study in the validation stage. DNA methylation was assessed using the Infinium Human Methylation450 BeadChip. NOX, NO2, PM10, PM2.5, PM coarse, traffic intensity and traffic load exposure were measured according to the ESCAPE protocol. A systematic review was undertaken to identify those cytosine-phosphate-guanine (CpGs) associated with air pollution in previous studies and we screened for them in the discovery study. Results: In the discovery stage of the epigenome-wide association study, 81 unique CpGs were associated with air pollution (p-value < 10(-5)) but none of them were validated in the replication sample. Furthermore, we identified 15 CpGs in the systematic review showing differential methylation with a p-value fulfilling the Bonferroni criteria and 1673 CpGs fulfilling the false discovery rate criteria, all of which were related to PM2.5 or NO2. None of them was replicated in the discovery study, in which the top hits were located in an intergenic region on chromosome 1 (cg10893043, p-value = 6.79.10(-5)) and in the LRRC45 and PXK genes (cg05088605, p-value = 2.15.10(-04); cg16560256, p-value = 2.23.10(-04)). Conclusions: Neither new genomic loci associated with long-term air pollution were identified, nor previously identified loci were replicated. Continued efforts to test this potential association are warranted. | Notes: | Elosua, R (reprint author), Hosp Mar Med Res Inst, Dr Aiguader 88, Barcelona 08003, Catalonia, Spain. relosua@imim.es |
Keywords: | Air pollution; DNA methylation; Epigenome-wide association study | Document URI: | http://hdl.handle.net/1942/31386 | ISSN: | 0013-9351 | e-ISSN: | 1096-0953 | DOI: | 10.1016/j.envres.2019.108550 | ISI #: | WOS:000483410200025 | Rights: | 2019 Elsevier Inc. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sayols_Baixeras_Sergi_2019.pdf Restricted Access | Published version | 292.58 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
2
checked on Sep 5, 2020
WEB OF SCIENCETM
Citations
17
checked on Oct 12, 2024
Page view(s)
22
checked on Sep 7, 2022
Download(s)
4
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.