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Title: | Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes | Authors: | Ribeiro, Rita Sofia Garcia Ketkar-Atre, Ashwini Yin, Ting Louchami, Karim STRUYS, Tom LAMBRICHTS, Ivo Sener, Abdullah Malaisse, Willy Jean De Cuyper, Marcel Himmelreich, Uwe |
Issue Date: | 2018 | Publisher: | MDPI | Source: | JOURNAL OF PERSONALIZED MEDICINE, 8 (1) (Art N° 12) | Abstract: | Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs. It was possible to incorporate Magnetic Ressonance (MR)-detectable amounts of MLs in a shorter time (4 h) when compared to Endorem and Resovist. MLs did not show negative effects on the PIs' viability and functional parameters in vitro. Labeled islets were transplanted in the renal sub-capsular region of healthy mice. Hypointense contrast in MR images due to the labeled PIs was detected in vivo upon transplantation, while MR detection of PIs labeled with Endorem and Resovist was only possible after the addition of transfection agents. These findings indicate that MLs are suitable to image PIs, without affecting their function, which is promising for future longitudinal pre-clinical and clinical studies involving the assessment of PI transplantation. | Notes: | Himmelreich, U (reprint author), Univ Leuven, Dept Imaging & Pathol, Biomed MRI MoSAIC, Herestr 49, B-3000 Leuven, Belgium. Rita.Sofia.Garcia.Ribeiro@vub.ac.be; ashket2982@gmail.com; yinting527@gmail.com; karim.louchami@ulb.ac.be; tom.struys@sprofit.com; ivo.lambrichts@uhasselt.be; abdsener@ulb.ac.be; malaisse@ulb.ac.be; marcel.decuyper@kuleuven.be; Uwe.Himmelreich@med.kuleuven.be |
Keywords: | pancreatic islets; islet transplantation; MRI; contrast agents;;magnetoliposomes; SPIOs; insulin; diabetes mellitus; cell imaging;;transmission electron microscopy | Document URI: | http://hdl.handle.net/1942/31389 | e-ISSN: | 2075-4426 | DOI: | 10.3390/jpm8010012 | ISI #: | WOS:000455273600011 | Rights: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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jpm-08-00012.pdf | Published version | 3.11 MB | Adobe PDF | View/Open |
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