Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/313
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heirwegh, Karel P. M. | - |
dc.contributor.author | Vermeir, Marc | - |
dc.contributor.author | MOLENBERGHS, Geert | - |
dc.date.accessioned | 2004-10-22T11:16:04Z | - |
dc.date.available | 2004-10-22T11:16:04Z | - |
dc.date.issued | 1993 | - |
dc.identifier.citation | Journal of Biochemical and Biophysical Methods, 27(2), p. 151-156 | - |
dc.identifier.issn | 0165-022X | - |
dc.identifier.uri | http://hdl.handle.net/1942/313 | - |
dc.description.abstract | Saturation curves for substrate interaction with an acceptor (enzyme, binding or carrier protein) are often analysed on the assumption that the amount of acceptor-bound substrate is negligible compare to its total amount. Analytical criteria permitting one to decide whether the assumption is justified or not for systems described by a single Michaelis-Menten equation have been derived previously. For more complicated systems, error formulae often cannot be obtained in closed form and, if obtainable, are unwieldy. How this more complicated problem can be tackled is shown for mixtures of acceptor sites described by a sum of several Michaelis-Menten terms, without or with an additional term for ‘non-specific’ uptake or binding. In particular, it is shown that the maximum error, for which simple analytical expressions are obtained, provides a valid criterion for assessing whether substrates depletion is negligible or not | - |
dc.description.sponsorship | This work has been supported by the National Fund for Scientific Research of Belgium. | - |
dc.language.iso | en | - |
dc.rights | © 1993 Elsevier Science Publishers B.V. All rights reserved | - |
dc.subject | Medical statistics | - |
dc.subject.other | substrate-depletion error; enzymic conversion; transport kinetics; equilibrium binding; receptor binding | - |
dc.title | The Substrate-Depletion Error on the Multiple Michaelis-Menten Equation with and without an Added Linear Term in the Substrate Concentration | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 156 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 151 | - |
dc.identifier.volume | 27 | - |
local.type.refereed | Refereed | - |
local.type.specified | Note | - |
dc.bibliographicCitation.oldjcat | - | |
dc.identifier.doi | 10.1016/0165-022X(93)90059-W | - |
dc.identifier.isi | A1993LX81500008 | - |
item.fulltext | With Fulltext | - |
item.fullcitation | Heirwegh, Karel P. M.; Vermeir, Marc & MOLENBERGHS, Geert (1993) The Substrate-Depletion Error on the Multiple Michaelis-Menten Equation with and without an Added Linear Term in the Substrate Concentration. In: Journal of Biochemical and Biophysical Methods, 27(2), p. 151-156. | - |
item.accessRights | Restricted Access | - |
item.contributor | Heirwegh, Karel P. M. | - |
item.contributor | Vermeir, Marc | - |
item.contributor | MOLENBERGHS, Geert | - |
crisitem.journal.issn | 0165-022X | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
heirwegh1993.pdf Restricted Access | Published version | 299.08 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
1
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
1
checked on May 23, 2024
Page view(s)
24
checked on Sep 6, 2022
Download(s)
8
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.