Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/31451
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tamman, Hedvig | - |
dc.contributor.author | Van Nerom, Katleen | - |
dc.contributor.author | Takada, Hiraku | - |
dc.contributor.author | Vandenberk, Niels | - |
dc.contributor.author | Scholls, Daniel | - |
dc.contributor.author | Polikanov, Yury | - |
dc.contributor.author | Hofkens, Johan | - |
dc.contributor.author | Talavera, Ariel | - |
dc.contributor.author | Hauryliuk, Vasili | - |
dc.contributor.author | HENDRIX, Jelle | - |
dc.contributor.author | Garcia-Pino, Abel | - |
dc.date.accessioned | 2020-07-08T14:43:43Z | - |
dc.date.available | 2020-07-08T14:43:43Z | - |
dc.date.issued | 2020 | - |
dc.date.submitted | 2020-07-07T13:42:13Z | - |
dc.identifier.citation | NATURE CHEMICAL BIOLOGY, 16 (8), p. 834–840 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | http://hdl.handle.net/1942/31451 | - |
dc.description.abstract | Bifunctional Rel stringent factors, the most abundant class of RelA/SpoT homologs, are ribosome-associated enzymes that transfer a pyrophosphate from ATP onto the 3 ' of guanosine tri-/diphosphate (GTP/GDP) to synthesize the bacterial alarmone (p)ppGpp, and also catalyze the 3 ' pyrophosphate hydrolysis to degrade it. The regulation of the opposing activities of Rel enzymes is a complex allosteric mechanism that remains an active research topic despite decades of research. We show that a guanine-nucleotide-switch mechanism controls catalysis by Thermus thermophilus Rel (Rel(Tt)). The binding of GDP/ATP opens the N-terminal catalytic domains (NTD) of Rel(Tt) (Rel(Tt)(NTD)) by stretching apart the two catalytic domains. This activates the synthetase domain and allosterically blocks hydrolysis. Conversely, binding of ppGpp to the hydrolase domain closes the NTD, burying the synthetase active site and precluding the binding of synthesis precursors. This allosteric mechanism is an activity switch that safeguards against futile cycles of alarmone synthesis and degradation. | - |
dc.description.sponsorship | We acknowledge the use of the synchrotron-radiation facility at the SOLEIL synchrotron Gif-sur-Yvette, France, under proposals 20150717, 20160750 and 20170756. We also thank the staff from Swing, PROXIMA-1 and PROXIMA-2A beamlines at SOLEIL for assistance with data collection. This work was supported by grants from the Fonds National de Recherche Scientifique, nos. FNRS-EQP U.N043.17F, FRFS-WELBIO CR-2017S-03 and FNRS-PDR T.0066.18, and the Joint Programming Initiative on Antimicrobial Resistance (grant no. JPI-EC-AMR-R.8004.18-) to A.G.-P. The Program 'Actions de Recherche Concerte'e' 2016-2021 and Fonds d'Encouragement a la Recherche from the ULB, Fonds Jean Brachet and the Fondation Van Buren to A.G.-P.; the Molecular Infection Medicine Sweden, Swedish Research council (grant no. 2017-03783), and Ragnar Soderberg foundation fellowship to V.H.; J. Hendrix and J. Hofkens are grateful to the Research Foundation Flanders (FWO Vlaanderen, grant no. G0B4915N) and large infrastructure grant (no. ZW15_09 GOH6316N) and the KU Leuven Research Fund (no. C14/16/053); J.Hofkens thanks financial support of the Flemish government through long-term structural funding Methusalem (CASAS2, Meth/15/04). K.V.N. was supported by a PhD grant from the Fonds National de Recherche Scientifique FNRS-FRIA. N.V. acknowledges the Agency for Innovation by Science and Technology in Flanders for a PhD grant. H. Tamman was supported by a Charge de Recherches fellowship from the FNRS (no. CR/DM-392). H. Takada was supported by the postdoctoral grant from the Umeao Centre for Microbial Research (UCMR). | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.rights | 2020 Springer Nature Limited. | - |
dc.subject.other | Single-Molecule Fret | - |
dc.subject.other | (P)Ppgpp | - |
dc.subject.other | Protein | - |
dc.subject.other | Synthetase | - |
dc.subject.other | Refinement | - |
dc.subject.other | Precision | - |
dc.subject.other | Ribosome | - |
dc.subject.other | Homolog | - |
dc.subject.other | Domain | - |
dc.subject.other | Ppgpp | - |
dc.title | A nucleotide-switch mechanism mediates opposing catalytic activities of Rel enzymes | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 840 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 834 | - |
dc.identifier.volume | 16 | - |
local.format.pages | 7 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Garcia-Pino, A (reprint author), Univ Libre Bruxelles, Fac Sci, Cellular & Mol Microbiol, Brussels, Belgium.; Hauryliuk, V (reprint author), Umea Univ, Dept Mol Biol, Umea, Sweden.; Hauryliuk, V (reprint author), Umea Univ, Lab Mol Infect Med Sweden, Umea, Sweden.; Hendrix, J (reprint author), Katholieke Univ Leuven, Chem Dept, Mol Imaging & Photon, Leuven, Belgium.; Garcia-Pino, A (reprint author), WELBIO, Brussels, Belgium. | - |
dc.description.notes | vasili.hauryliuk@umu.se; jelle.hendrix@uhasselt.be; agarciap@ulb.ac.be | - |
dc.description.other | Garcia-Pino, A (corresponding author), Univ Libre Bruxelles, Fac Sci, Cellular & Mol Microbiol, Brussels, Belgium; WELBIO, Brussels, Belgium. Hauryliuk, V (corresponding author), Umea Univ, Dept Mol Biol, Umea, Sweden; Umea Univ, Lab Mol Infect Med Sweden, Umea, Sweden. Hendrix, J (corresponding author), Katholieke Univ Leuven, Chem Dept, Mol Imaging & Photon, Leuven, Belgium. vasili.hauryliuk@umu.se; jelle.hendrix@uhasselt.be; agarciap@ulb.ac.be | - |
local.publisher.place | 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.source.type | Article | - |
dc.identifier.doi | 10.1038/s41589-020-0520-2 | - |
dc.identifier.pmid | 32393900 | - |
dc.identifier.isi | WOS:000531782600004 | - |
dc.contributor.orcid | Polikanov, Yury/0000-0002-5064-0327; Scholl, Daniel/0000-0002-5242-2032; | - |
dc.contributor.orcid | Hauryliuk, Vasili/0000-0003-2389-5057 | - |
dc.identifier.eissn | 1552-4469 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Tamman, Hedvig; Van Nerom, Katleen; Garcia-Pino, Abel] Univ Libre Bruxelles, Fac Sci, Cellular & Mol Microbiol, Brussels, Belgium. | - |
local.description.affiliation | [Takada, Hiraku; Hauryliuk, Vasili] Umea Univ, Dept Mol Biol, Umea, Sweden. | - |
local.description.affiliation | [Takada, Hiraku; Hauryliuk, Vasili] Umea Univ, Lab Mol Infect Med Sweden, Umea, Sweden. | - |
local.description.affiliation | [Vandenberk, Niels; Hofkens, Johan; Hendrix, Jelle] Katholieke Univ Leuven, Chem Dept, Mol Imaging & Photon, Leuven, Belgium. | - |
local.description.affiliation | [Scholls, Daniel] Univ Libre Bruxelles, SEMB, Brussels, Belgium. | - |
local.description.affiliation | [Polikanov, Yury] Univ Illinois, Coll Liberal Arts & Sci, Dept Biol Sci, Chicago, IL USA. | - |
local.description.affiliation | [Talavera, Ariel] Univ Libre Bruxelles, CMMI, Gosselies, Belgium. | - |
local.description.affiliation | [Hendrix, Jelle] Hasselt Univ, Dynam Bioimaging Lab, Adv Opt Microscopy Ctr, Agoralaan C BIOMED, Hasselt, Belgium. | - |
local.description.affiliation | [Hendrix, Jelle] Hasselt Univ, Biomed Res Inst, Agoralaan C BIOMED, Hasselt, Belgium. | - |
local.description.affiliation | [Garcia-Pino, Abel] WELBIO, Brussels, Belgium. | - |
local.uhasselt.international | yes | - |
item.fullcitation | Tamman, Hedvig; Van Nerom, Katleen; Takada, Hiraku; Vandenberk, Niels; Scholls, Daniel; Polikanov, Yury; Hofkens, Johan; Talavera, Ariel; Hauryliuk, Vasili; HENDRIX, Jelle & Garcia-Pino, Abel (2020) A nucleotide-switch mechanism mediates opposing catalytic activities of Rel enzymes. In: NATURE CHEMICAL BIOLOGY, 16 (8), p. 834–840. | - |
item.validation | ecoom 2021 | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
item.contributor | Tamman, Hedvig | - |
item.contributor | Van Nerom, Katleen | - |
item.contributor | Takada, Hiraku | - |
item.contributor | Vandenberk, Niels | - |
item.contributor | Scholls, Daniel | - |
item.contributor | Polikanov, Yury | - |
item.contributor | Hofkens, Johan | - |
item.contributor | Talavera, Ariel | - |
item.contributor | Hauryliuk, Vasili | - |
item.contributor | HENDRIX, Jelle | - |
item.contributor | Garcia-Pino, Abel | - |
crisitem.journal.issn | 1552-4450 | - |
crisitem.journal.eissn | 1552-4469 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
s41589-020-0520-2 (2).pdf Restricted Access | Published version | 4.68 MB | Adobe PDF | View/Open Request a copy |
WEB OF SCIENCETM
Citations
30
checked on Jul 18, 2024
Page view(s)
34
checked on Sep 7, 2022
Download(s)
6
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.