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http://hdl.handle.net/1942/31471
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DC Field | Value | Language |
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dc.contributor.author | D'ONOFRIO, Valentino | - |
dc.contributor.author | Conzemius, Rick | - |
dc.contributor.author | Varda-Brkic, Dijana | - |
dc.contributor.author | Bogdan, Maja | - |
dc.contributor.author | Grisold, Andrea | - |
dc.contributor.author | GYSSENS, Inge | - |
dc.contributor.author | Bedenic, Branka | - |
dc.contributor.author | Barisic, Ivan | - |
dc.date.accessioned | 2020-07-16T10:23:15Z | - |
dc.date.available | 2020-07-16T10:23:15Z | - |
dc.date.issued | 2020 | - |
dc.date.submitted | 2020-07-08T10:55:05Z | - |
dc.identifier.citation | INFECTION GENETICS AND EVOLUTION, 81 (Art N° 104263) | - |
dc.identifier.issn | 1567-1348 | - |
dc.identifier.uri | http://hdl.handle.net/1942/31471 | - |
dc.description.abstract | Colistin is a last-resort antibiotic for the treatment of infections caused by multidrug and carbapenem-resistant Gram-negative bacteria. Colistin resistance has been emerging and multiple outbreaks have been reported in Europe and elsewhere. It has been most frequently reported in carbapenem-resistant K. pneumoniae. In this study, 24 multidrug and colistin-resistant clinical isolates (14 K. pneumoniae, one E. aerogenes, one E. cloacae, and eight A. baumannii) were collected from four hospitals in Croatia from 2013 to 2018, in order to analyse the molecular epidemiology and mechanisms of antibiotic resistance. beta-lactamase and carbapenemase genes were detected by PCR. Genotyping was done on selected isolates by rep-PCR. Whole genome sequencing (WGS) was performed to discover possible molecular mechanisms for the observed colistin resistance. All isolates, except two K. pneumoniae isolates, were extensively drug resistant. Ten out of 16 (63%) K. pneumoniae isolates possessed bla(OXA-48), which is the most common carbapenem resistance gene in Croatia and in other parts of Europe. All A. baumannii isolates possessed the OXA-23-like carbapenem hydrolysing oxacillinase and five turned out to be pandrug-resistant. Colistin resistance was most likely chromosomally mediated. After sequence analysis, none of the isolates were found to possess any of the mcr gene variants. Several previously reported mutations were found in PmrB, PhoP, PhoQ, and MgrB, which are associated with colistin resistance. In the global phylogenetic analysis, DNA mutations causing mutations in the MgrB protein were present mostly in lineages comprising colistin resistant isolates, and the second most prevalent mutation (K3X) was also encountered in our isolates. In addition, based on genotyping by rep-PCR, the spread of colistin resistance is most likely to be clonal. Most importantly, the presence of colistin resistance together with carbapenemase genes in extensively drug resistant isolates poses real threats in the use of carbapenems and colistin to fight infections. | - |
dc.description.sponsorship | This project has received funding from the European Union's Horizon 2020 research and innovation program under GA 634137. This study is part of the Limburg Clinical Research Program (LCRP) UHasselt-ZOL-Jessa, supported by the foundation Limburg Sterk Merk (LSM), Hasselt University, Ziekenhuis Oost-Limburg and Jessa Hospital. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.rights | 2020 Elsevier B.V. All rights reserved. | - |
dc.subject.other | Carbapenemase | - |
dc.subject.other | Colistin resistance | - |
dc.subject.other | Enterobacteriaceae | - |
dc.subject.other | A. baumannii | - |
dc.title | Epidemiology of colistin-resistant, carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in Croatia | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 81 | - |
local.format.pages | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Barisic, I (reprint author), Austrian Inst Technol, AIT, A-1210 Vienna, Austria. | - |
dc.description.notes | Ivan.Barisic@ait.ac.at | - |
dc.description.other | Barisic, I (corresponding author), Austrian Inst Technol, AIT, A-1210 Vienna, Austria. Ivan.Barisic@ait.ac.at | - |
local.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | - |
dc.relation.references | , | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 104263 | - |
dc.source.type | Article | - |
dc.identifier.doi | 10.1016/j.meegid.2020.104263 | - |
dc.identifier.pmid | 32105865 | - |
dc.identifier.isi | WOS:000532689800002 | - |
dc.contributor.orcid | Conzemius, Rick/0000-0002-7116-8180; D'Onofrio, | - |
dc.contributor.orcid | Valentino/0000-0003-3828-0442 | - |
dc.identifier.eissn | 1567-7257 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [D'Onofrio, Valentino; Gyssens, Inge C.] Hasselt Univ, Fac Med & Life Sci, B-3500 Hasselt, Belgium. | - |
local.description.affiliation | [D'Onofrio, Valentino] Jessa Hosp, Dept Infect Dis & Immun, B-3500 Hasselt, Belgium. | - |
local.description.affiliation | [D'Onofrio, Valentino; Gyssens, Inge C.] Radboud Univ Nijmegen, Dept Internal Med, Med Ctr, NL-6663 Nijmegen, Netherlands. | - |
local.description.affiliation | [D'Onofrio, Valentino; Gyssens, Inge C.] Radboud Univ Nijmegen, Med Ctr, Ctr Infect Dis, NL-6663 Nijmegen, Netherlands. | - |
local.description.affiliation | [Conzemius, Rick; Barisic, Ivan] Austrian Inst Technol, AIT, A-1210 Vienna, Austria. | - |
local.description.affiliation | [Varda-Brkic, Dijana; Bedenic, Branka] Univ Hosp Ctr Zagreb, Zagreb 10000, Croatia. | - |
local.description.affiliation | [Bogdan, Maja] Publ Hlth Inst Osijek Baranja Cty, Osijek 31000, Croatia. | - |
local.description.affiliation | [Bogdan, Maja] Univ Osijek, Sch Med, Osijek 31000, Croatia. | - |
local.description.affiliation | [Grisold, Andrea] Med Univ Graz, Inst Hyg Microbiol & Environm Med, Graz, Austria. | - |
local.description.affiliation | [Bedenic, Branka] Univ Zagreb, Sch Med, Zagreb 10000, Croatia. | - |
local.uhasselt.international | yes | - |
item.validation | ecoom 2021 | - |
item.contributor | D'ONOFRIO, Valentino | - |
item.contributor | Conzemius, Rick | - |
item.contributor | Varda-Brkic, Dijana | - |
item.contributor | Bogdan, Maja | - |
item.contributor | Grisold, Andrea | - |
item.contributor | GYSSENS, Inge | - |
item.contributor | Bedenic, Branka | - |
item.contributor | Barisic, Ivan | - |
item.accessRights | Restricted Access | - |
item.fullcitation | D'ONOFRIO, Valentino; Conzemius, Rick; Varda-Brkic, Dijana; Bogdan, Maja; Grisold, Andrea; GYSSENS, Inge; Bedenic, Branka & Barisic, Ivan (2020) Epidemiology of colistin-resistant, carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in Croatia. In: INFECTION GENETICS AND EVOLUTION, 81 (Art N° 104263). | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 1567-1348 | - |
crisitem.journal.eissn | 1567-7257 | - |
Appears in Collections: | Research publications |
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valentino.pdf Restricted Access | Published version | 1.65 MB | Adobe PDF | View/Open Request a copy |
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