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http://hdl.handle.net/1942/31776
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DC Field | Value | Language |
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dc.contributor.author | Wagemans, J | - |
dc.contributor.author | Tsonos, J | - |
dc.contributor.author | Holtappels, D | - |
dc.contributor.author | Fortuna, K | - |
dc.contributor.author | Hernalsteens, JP | - |
dc.contributor.author | De Greve, H | - |
dc.contributor.author | Estrozi, LF | - |
dc.contributor.author | Bacia-Verloop, M | - |
dc.contributor.author | Moriscot, C | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Schoehn, G | - |
dc.contributor.author | Lavigne, R | - |
dc.date.accessioned | 2020-08-21T09:11:50Z | - |
dc.date.available | 2020-08-21T09:11:50Z | - |
dc.date.issued | 2020 | - |
dc.date.submitted | 2020-08-18T15:34:04Z | - |
dc.identifier.citation | International journal of molecular sciences (Print), 21 (9) , p. 3119 (Art N° 3119) | - |
dc.identifier.uri | http://hdl.handle.net/1942/31776 | - |
dc.description.abstract | The phAPEC6 genome encodes 551 predicted gene products, with the vast majority (83%) of unknown function. Of these, 62 have been identified as virion-associated proteins by mass spectrometry (ESI-MS/MS), including the major capsid protein (Gp225; present in 1620 copies), which shows a HK97 capsid protein-based fold. Cryo-electron microscopy experiments showed that the 350-kbp DNA molecule of Escherichia coli virus phAPEC6 is packaged in at least 15 concentric layers in the phage capsid. A capsid inner body rod is also present, measuring about 91 nm by 18 nm and oriented along the portal axis. In the phAPEC6 contractile tail, 25 hexameric stacked rings can be distinguished, built of the identified tail sheath protein (Gp277). Cryo-EM reconstruction reveals the base of the unique hairy fibers observed during an initial transmission electron microscopy (TEM) analysis. These very unusual filaments are ordered at three annular positions along the contractile sheath, as well as around the capsid, and may be involved in host interaction. | - |
dc.description.sponsorship | This work used the platforms of the Grenoble Instruct-ERIC centre (ISBG; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). The electronmicroscope facilityis supported by the Auvergne-Rhône-Alpes Region, the Fondation Recherche Médicale (FRM), the fonds FEDER and the GIS-Infrastructures en Biologie Santé et Agronomie (IBISA). IBS acknowledges integration into the Interdisciplinary Research Institute of Grenoble (IRIG, CEA). This study was also supported by the AntibioPhage Project, funded by an Animal Health and Welfare ERA-Net (ANIHWA) grant from the EU FP-Horizon 2020 Programme and by grant RT 09/8 APECON from the Federal Public Services of Health, Food Chain Safety and Environment (Belgium) | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.rights | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | - |
dc.subject.other | cryo-EM | - |
dc.subject.other | jumbo phage | - |
dc.subject.other | HK97-fold | - |
dc.title | Structural Analysis of Jumbo Coliphage phAPEC6 | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 3119 | - |
dc.identifier.volume | 21 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 3119 | - |
dc.identifier.doi | 10.3390/ijms21093119 | - |
dc.identifier.pmid | 32354127 | - |
dc.identifier.isi | WOS:000535581700090 | - |
dc.identifier.eissn | - | |
local.provider.type | Web of Science | - |
local.uhasselt.uhpub | yes | - |
item.validation | ecoom 2021 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.fullcitation | Wagemans, J; Tsonos, J; Holtappels, D; Fortuna, K; Hernalsteens, JP; De Greve, H; Estrozi, LF; Bacia-Verloop, M; Moriscot, C; NOBEN, Jean-Paul; Schoehn, G & Lavigne, R (2020) Structural Analysis of Jumbo Coliphage phAPEC6. In: International journal of molecular sciences (Print), 21 (9) , p. 3119 (Art N° 3119). | - |
item.contributor | Wagemans, J | - |
item.contributor | Tsonos, J | - |
item.contributor | Holtappels, D | - |
item.contributor | Fortuna, K | - |
item.contributor | Hernalsteens, JP | - |
item.contributor | De Greve, H | - |
item.contributor | Estrozi, LF | - |
item.contributor | Bacia-Verloop, M | - |
item.contributor | Moriscot, C | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Schoehn, G | - |
item.contributor | Lavigne, R | - |
crisitem.journal.issn | 1661-6596 | - |
crisitem.journal.eissn | 1422-0067 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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ijms-21-03119-v2.pdf | Published version | 3.39 MB | Adobe PDF | View/Open |
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