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Title: | Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii | Authors: | Gambino, Michela Nørgaard Sørensen, Anders Ahern, Stephen Smyrlis, Georgios Gencay, Yilmaz Emre Hendrix, Hanne Neve, Horst NOBEN, Jean-Paul Lavigne, Rob Brøndsted, Lone |
Issue Date: | 2020 | Publisher: | MDPI | Source: | International journal of molecular sciences (Print), 21 (15) (Art N° 5196) | Abstract: | Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter sakazakii. Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144 tail fiber structure is, overall, similar to the tail fiber of phage Mu and the C-terminus shows amino acid similarity to tail fibers of otherwise unrelated phages infecting Cronobacter. Since all phages in the Loughboroughvirus genus encode tail fibers similar to S144, we suggest that phages in this genus infect Cronobacter sakazakii and are polyvalent | Keywords: | phage;polyvalent;Enterobacteriaceae | Document URI: | http://hdl.handle.net/1942/31778 | ISSN: | 1661-6596 | e-ISSN: | 1422-0067 | DOI: | 10.3390/ijms21155196 | ISI #: | WOS:000559271800001 | Rights: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
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ijms-21-05196-v2.pdf | Published version | 17.36 MB | Adobe PDF | View/Open |
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