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Title: | Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds | Authors: | GULKA, Michal Salehi, Hamideh Varga, Bela Middendorp, Elodie Pall, Orsolya Raabova, Helena Cloitre, Thierry Cuisinier, Frederic J. G. Cigler, Petr NESLADEK, Milos Gergely, Csilla |
Issue Date: | 2020 | Publisher: | NATURE PUBLISHING GROUP | Source: | SCIENTIFIC REPORTS, 10 (1) (Art N° 9791) | Abstract: | In recent years, fluorescent nanodiamond (fND) particles containing nitrogen-vacancy (NV) centers gained recognition as an attractive probe for nanoscale cellular imaging and quantum sensing. For these applications, precise localization of fNDs inside of a living cell is essential. Here we propose such a method by simultaneous detection of the signal from the NV centers and the spectroscopic Raman signal from the cells to visualize the nucleus of living cells. However, we show that the commonly used Raman cell signal from the fingerprint region is not suitable for organelle imaging in this case. Therefore, we develop a method for nucleus visualization exploiting the region-specific shape of C-H stretching mode and further use k-means cluster analysis to chemically distinguish the vicinity of fNDs. Our technique enables, within a single scan, to detect fNDs, distinguish by chemical localization whether they have been internalized into cell and simultaneously visualize cell nucleus without any labeling or cell-fixation. We show for the first time spectral colocalization of unmodified high-pressure high-temperature fND probes with the cell nucleus. Our methodology can be, in principle, extended to any red- and near-infrared-luminescent cell-probes and is fully compatible with quantum sensing measurements in living cells. | Notes: | Gulka, M (corresponding author), Hasselt Univ, Inst Mat Res IMO, Wetenschapspk 1, B-3590 Diepenbeek, Belgium.; Gulka, M (corresponding author), Czech Tech Univ, Fac Biomed Engn, Dept Biomed Technol, Sitna Sq 3105, Kladno 27201, Czech Republic. gulka.michal@gmail.com |
Other: | Gulka, M (corresponding author), Hasselt Univ, Inst Mat Res IMO, Wetenschapspk 1, B-3590 Diepenbeek, Belgium; Czech Tech Univ, Fac Biomed Engn, Dept Biomed Technol, Sitna Sq 3105, Kladno 27201, Czech Republic. gulka.michal@gmail.com | Keywords: | Fluorescent Nanodiamonds;Raman-Spectroscopy;Mass-Production;Dna;Nanoparticles;Single;Transport;Tracking;Centers;Probes | Document URI: | http://hdl.handle.net/1942/31967 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-020-66593-7 | ISI #: | WOS:000543956500031 | Rights: | © Te Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
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michal.pdf | Published version | 4.57 MB | Adobe PDF | View/Open |
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