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http://hdl.handle.net/1942/32402
Title: | Imaging the Replication of Single Viruses: Lessons Learned from HIV and Future Challenges To Overcome | Authors: | Rocha, Susana HENDRIX, Jelle Borrenberghs, Doortje Debyser, Zeger Hofkens, Johan |
Issue Date: | 2020 | Source: | ACS nano, 14 (9) , p. 10775 -10783 | Abstract: | The molecular composition of viral particles indicates
that a single virion is capable of initiating an infection. However, the
majority of viruses that come into contact with cells fails to infect them.
Understanding what makes one viral particle more successful than
others requires visualizing the infection process directly in living cells,
one virion at a time. In this Perspective, we explain how single-virus
imaging using fluorescence microscopy can provide answers to
unsolved questions in virology. We discuss fluorescent labeling of
virus particles, resolution at the subviral and molecular levels, tracking
in living cells, and imaging of interactions between viral and host
proteins. We end this Perspective with a set of remaining questions in
understanding the life cycle of retroviruses and how imaging a single
virus can help researchers address these questions. Although we use
examples from the HIV field, these methods are of value for the study of other viruses as well. The molecular composition of viral particles indicates that a single virion is capable of initiating an infection. However, the majority of viruses that come into contact with cells fails to infect them. Understanding what makes one viral particle more successful than others requires visualizing the infection process directly in living cells, one virion at a time. In this Perspective, we explain how single-virus imaging using fluorescence microscopy can provide answers to unsolved questions in virology. We discuss fluorescent labeling of virus particles, resolution at the subviral and molecular levels, tracking in living cells, and imaging of interactions between viral and host proteins. We end this Perspective with a set of remaining questions in understanding the life cycle of retroviruses and how imaging a single virus can help researchers address these questions. Although we use examples from the HIV field, these methods are of value for the study of other viruses as well. |
Document URI: | http://hdl.handle.net/1942/32402 | ISSN: | 1936-0851 | e-ISSN: | 1936-086X | DOI: | 10.1021/acsnano.0c06369 | ISI #: | WOS:000615914200001 | Rights: | 2020 American Chemical Society | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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acsnano 0c06369.pdf Restricted Access | Published version | 2.12 MB | Adobe PDF | View/Open Request a copy |
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