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http://hdl.handle.net/1942/32472
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DC Field | Value | Language |
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dc.contributor.author | VOS, Stijn | - |
dc.contributor.author | NAWROT, Tim | - |
dc.contributor.author | MARTENS, Dries | - |
dc.contributor.author | Hyang-Min Byun | - |
dc.contributor.author | JANSSEN, Bram | - |
dc.date.accessioned | 2020-10-15T09:23:58Z | - |
dc.date.available | 2020-10-15T09:23:58Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2020-09-09T11:29:02Z | - |
dc.identifier.citation | EPIGENETICS, 16(2), p. 121-131 | - |
dc.identifier.uri | http://hdl.handle.net/1942/32472 | - |
dc.description.abstract | While previous studies have demonstrated that prenatal exposure to environmental stressors is associated with mitochondrial DNA (mtDNA) methylation, more recent investigations are questioning the accuracy of the methylation assessment and its biological relevance. In this study, we investigated placental mtDNA methylation while accounting for methodological issues such as nuclear contamination, bisulphite conversion, and PCR bias. From the ENVIRONAGE birth cohort, we selected three groups of participants (n = 20/group). One group with mothers who smoked during pregnancy (average 13.2 cig/day), one group with high air pollutant exposure (PM2.5: 16.0 +/- 1.4 mu g/m(3), black carbon: 1.8 +/- 0.3 mu g/m(3)) and one control group (non-smokers, PM2.5: 10.6 +/- 1.7 mu g/m(3), black carbon: 0.9 +/- 0.1 mu g/m(3)) with low air pollutant exposure. DNA methylation levels were quantified in two regions of the displacement loop control region (D-loopandLDLR2) by bisulphite pyrosequencing. Additionally, we measured DNA methylation on nuclear genes involved in mitochondrial maintenance (PINK1, DNA2, andPOLG1) and assessed mtDNA content using qPCR. AbsoluteD-loopmethylation levels were higher for mothers that smoked extensively (+0.36%, 95% CI: 0.06% to 0.66%), and for mothers that were highly exposed to air pollutants (+0.47%, 95% CI: 0.20% to 0.73%). The relevance of our findings is further supported, asD-loopmethylation levels were correlated with placental mtDNA content (r = -0.40, p = 0.002) and associated with birth weight (-106.98 g, 95% CI: -209.60 g to -4.36 g for an IQR increase inD-loopmethylation). Most notably, our data demonstrates relevant levels of mtDNA methylation in placenta tissue, with significant associations between prenatal exposure to environmental stressors andD-loopmethylation. | - |
dc.description.sponsorship | This work was supported by the Research Foundation Flanders [G082317N]; Research Foundation Flanders [N1518119]. | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.rights | © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-ncnd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. | - |
dc.subject.other | Mitochondria | - |
dc.subject.other | mtDNA | - |
dc.subject.other | methylation | - |
dc.subject.other | placenta | - |
dc.subject.other | air pollution | - |
dc.subject.other | smoking | - |
dc.subject.other | prenatal | - |
dc.subject.other | DOHaD | - |
dc.subject.other | markers | - |
dc.subject.other | birthweight | - |
dc.title | Mitochondrial DNA methylation in placental tissue: a proof of concept study by means of prenatal environmental stressors | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 131 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 121 | - |
dc.identifier.volume | 16 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Janssen, BG (corresponding author), Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. | - |
dc.description.notes | bram.janssen@uhasselt.be | - |
dc.description.other | Janssen, BG (corresponding author), Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. bram.janssen@uhasselt.be | - |
local.publisher.place | 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1080/15592294.2020.1790923 | - |
dc.identifier.pmid | 32657220 | - |
dc.identifier.isi | WOS:000547731200001 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Vos, Stijn; Nawrot, Tim S.; Martens, Dries S.; Janssen, Bram G.] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. | - |
local.description.affiliation | [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Occupat & Environm Med, Leuven, Belgium. | - |
local.description.affiliation | [Hyang-Min Byun] Newcastle Univ, Populat Hlth Sci Inst, Newcastle Upon Tyne, Tyne & Wear, England. | - |
local.uhasselt.international | yes | - |
item.fullcitation | VOS, Stijn; NAWROT, Tim; MARTENS, Dries; Hyang-Min Byun & JANSSEN, Bram (2021) Mitochondrial DNA methylation in placental tissue: a proof of concept study by means of prenatal environmental stressors. In: EPIGENETICS, 16(2), p. 121-131. | - |
item.contributor | VOS, Stijn | - |
item.contributor | NAWROT, Tim | - |
item.contributor | MARTENS, Dries | - |
item.contributor | Hyang-Min Byun | - |
item.contributor | JANSSEN, Bram | - |
item.validation | ecoom 2021 | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 1559-2294 | - |
crisitem.journal.eissn | 1559-2308 | - |
Appears in Collections: | Research publications |
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Mitochondrial DNA methylation in placental tissue a proof of concept study by means of prenatal environmental stressors.pdf | Published version | 904.73 kB | Adobe PDF | View/Open |
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