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Title: | Non-muscle invasive bladder cancer tissues have increased base excision repair capacity | Authors: | Somuncu, Berna Keskin, Selcuk Antmen, Fatma Merve Saglican, Yesim Ekmekcioglu, Aysegul Ertuzun, Tugce Tuna, Mustafa Bilal Obek, Can WILSON, David Ince, Umit Kural, Ali Riza Muftuoglu, Meltem |
Issue Date: | 2020 | Publisher: | NATURE RESEARCH | Source: | SCIENTIFIC REPORTS, 10 (1) (Art N° 16371) | Abstract: | The molecular mechanisms underlying the development and progression of bladder cancer (BC) are complex and have not been fully elucidated. Alterations in base excision repair (BER) capacity, one of several DNA repair mechanisms assigned to preserving genome integrity, have been reported to influence cancer susceptibility, recurrence, and progression, as well as responses to chemotherapy and radiotherapy. We report herein that non-muscle invasive BC (NMIBC) tissues exhibit increased uracil incision, abasic endonuclease and gap-filling activities, as well as total BER capacity in comparison to normal bladder tissue from the same patient (p<0.05). No significant difference was detected in 8-oxoG incision activity between cancer and normal tissues. NMIBC tissues have elevated protein levels of uracil DNA glycosylase, 8-oxoguanine DNA glycosylase, AP endonuclease 1 and DNA polymerase beta protein. Moreover, the fold increase in total BER and the individual BER enzyme activities were greater in high-grade tissues than in low-grade NMIBC tissues. These findings suggest that enhanced BER activity may play a role in the etiology of NMIBC and that BER proteins could serve as biomarkers in disease prognosis, progression or response to genotoxic therapeutics, such as Bacillus Calmette-Guerin. | Notes: | Muftuoglu, M (corresponding author), Acibadem Mehmet Ali Aydinlar Univ, Dept Med Biotechnol, TR-34752 Istanbul, Turkey.; Muftuoglu, M (corresponding author), Acibadem Mehmet Ali Aydinlar Univ, Dept Mol Biol & Genet, TR-34752 Istanbul, Turkey. meltem.muftuoglu@acibadem.edu.tr |
Other: | Muftuoglu, M (corresponding author), Acibadem Mehmet Ali Aydinlar Univ, Dept Med Biotechnol, TR-34752 Istanbul, Turkey ; Acibadem Mehmet Ali Aydinlar Univ, Dept Mol Biol & Genet, TR-34752 Istanbul, Turkey. meltem.muftuoglu@acibadem.edu.tr | Keywords: | Dna-Polymerase-Beta;Apurinic/Apyrimidinic Endonuclease Activity;Genetic-Variants;Expression;Damage;Glycosylase;Overexpression;Risk;Ape1;Uracil | Document URI: | http://hdl.handle.net/1942/32783 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-020-73370-z | ISI #: | WOS:000577143400134 | Rights: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © Te Author(s) 2020 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2021 |
Appears in Collections: | Research publications |
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SomuncuB_2020.pdf | Published version | 6.45 MB | Adobe PDF | View/Open |
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