Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/32852
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Al-Khafaji, Alaa M. | - |
dc.contributor.author | Bell-Sakyi, Lesley | - |
dc.contributor.author | Fracasso, Gerardo | - |
dc.contributor.author | Luu, Lisa | - |
dc.contributor.author | HEYLEN, Dieter | - |
dc.contributor.author | Matthysen, Erik | - |
dc.contributor.author | Oteo, Jose A. | - |
dc.contributor.author | Palomar, Ana M. | - |
dc.date.accessioned | 2020-12-10T10:46:53Z | - |
dc.date.available | 2020-12-10T10:46:53Z | - |
dc.date.issued | 2020 | - |
dc.date.submitted | 2020-12-01T13:56:54Z | - |
dc.identifier.citation | Ticks and Tick-Borne Diseases, 11 (6) (Art N° 101511) | - |
dc.identifier.uri | http://hdl.handle.net/1942/32852 | - |
dc.description.abstract | Candidatus Rickettsia vini was originally detected in Ixodes arboricola ticks from Spain, and subsequently reported from several other Western Palearctic countries including Belgium. Recently, the bacterium was isolated in mammalian (Vero) cell culture from macerated male I. arboricola from Czech Republic, but there have been no reports of propagation in tick cells. Here we report isolation in a tick cell line of three strains of Ca. R. vini from I. arboricola collected from nests of great tits (Pants major) in Belgium. Internal organs of one male and two engorged female ticks were dissected aseptically, added to cultures of the Rhipicephalus microplus cell line BME/CTVM23 and incubated at 28 degrees C. Rickettsia-like bacteria were first seen in Giemsa-stained cytocentrifuge smears between 2 and 15 weeks later. Two of the isolates grew rapidly, destroying the tick cells within 2-4 weeks of onward passage in BME/CTVM23 cells, while the third isolate grew much more slowly, only requiring subculture at 4 - 5-month intervals. PCR amplification of bacterial 16S rRNA and Rickettsia gltA, sca4, ompB, ompA and 17-kDa genes revealed that all three isolates were Ca. R. vini, with 100 % identity to each other and to published Ca. R. vini sequences from other geographical locations. Transmission electron microscopy revealed typical single Rickettsia bacteria in the cytoplasm of BME/CTVM23 cells. The Ca. R. vini strain isolated from the male I. arboricola tick, designated Boshoek1, was tested for ability to grow in a panel of Ixodes ricinus, Ixodes scapularis and R. microplus cell lines and in Vero cells. The Boshoek1 strain grew rapidly, causing severe cytopathic effect, in the R. microplus line BME26, the I. ricinus line IRE11 and Vero cells, more slowly in the I. ricinus line IRE/CTVM19, possibly established a low-level infection in the I. ricinus line IRE/CTVM20, and failed to infect cells of any of four I. scapularis lines over a 12-week observation period. This study confirmed the applicability of the simple tick organ-cell line co-cultivation technique for isolation of tick-borne Rickettsia spp. using BME/CTVM23 cells. | - |
dc.description.sponsorship | We would like to thank the Tick Cell Biobank at the University of Liverpool and Timothy Kurtti and Ulrike Munderloh of the University of Minnesota for tick cell lines, and Alison Beckett, Aleksandra Beliavskaia and Catherine Hartley of the University of Liverpool for, respectively, assistance with transmission electron microscopy, photomicrographs and provision of uninfected Vero cells. LBS, AA-K and LL were funded by the UK Biotechnology and Biological Sciences Research Council grants BB/P024270/1 and BB/N023889/2; GF is funded by the Fund for Scientific Research (FWO) (Fonds voor Wetenschappelijk Onderzoek-Flanders, Belgium. Grant number G.0538.17) awarded to EM and DH; DH is funded by the Marie Sklodowska-Curie Actions (EUHorizon 2020, Individual Global Fellowship, project no 799609). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER GMBH | - |
dc.rights | © 2020 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | - |
dc.subject.other | Candidatus Rickettsia vini | - |
dc.subject.other | Ixodes arboricola | - |
dc.subject.other | Tree-hole tick | - |
dc.subject.other | Tick cell line | - |
dc.subject.other | Endosymbiont | - |
dc.subject.other | Parus major | - |
dc.title | Isolation of Candidatus Rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 6 | - |
dc.identifier.volume | 11 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Bell-Sakyi, L (corresponding author), Univ Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool Sci Pk IC2,146 Brownlow Hill, Liverpool L3 5RF, Merseyside, England. | - |
dc.description.notes | supervisore2@yahoo.com; L.Bell-Sakyi@liverpool.ac.uk; | - |
dc.description.notes | gerardo.fracasso@uantwerpen.be; lisaluu@liverpool.ac.uk; | - |
dc.description.notes | dieter.heylen@uantwerpen.be; erik.matthysen@uantwerpen.be; | - |
dc.description.notes | jaoteo@riojasalud.es; ampalomar@riojasalud.es | - |
dc.description.other | Bell-Sakyi, L (corresponding author), Univ Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool Sci Pk IC2,146 Brownlow Hill, Liverpool L3 5RF, Merseyside, England. supervisore2@yahoo.com; L.Bell-Sakyi@liverpool.ac.uk; gerardo.fracasso@uantwerpen.be; lisaluu@liverpool.ac.uk; dieter.heylen@uantwerpen.be; erik.matthysen@uantwerpen.be; jaoteo@riojasalud.es; ampalomar@riojasalud.es | - |
local.publisher.place | HACKERBRUCKE 6, 80335 MUNICH, GERMANY | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 101511 | - |
dc.identifier.doi | 10.1016/j.ttbdis.2020.101511 | - |
dc.identifier.isi | WOS:000576945200009 | - |
dc.contributor.orcid | Palomar, Ana M/0000-0002-5461-5874; Fracasso, | - |
dc.contributor.orcid | Gerardo/0000-0003-3384-4472 | - |
dc.identifier.eissn | - | |
dc.identifier.eissn | 1877-9603 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Al-Khafaji, Alaa M.; Bell-Sakyi, Lesley; Luu, Lisa] Univ Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool Sci Pk IC2,146 Brownlow Hill, Liverpool L3 5RF, Merseyside, England. | - |
local.description.affiliation | [Al-Khafaji, Alaa M.] Univ Al Qadisiyah, Coll Vet Med, Al Diwaniyah, Qadisiyah Provi, Iraq. | - |
local.description.affiliation | [Fracasso, Gerardo; Heylen, Dieter; Matthysen, Erik] Univ Antwerp, Dept Biol, Univ Pl 1, B-2610 Antwerp, Belgium. | - |
local.description.affiliation | [Heylen, Dieter] Princeton Univ, Dept Ecol & Evolutionary Biol, M26 Guyot Hall, Princeton, NJ 08544 USA. | - |
local.description.affiliation | [Heylen, Dieter] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Oteo, Jose A.; Palomar, Ana M.] Hosp Univ San Pedro CIBIR, Ctr Rickettsiosis & Arthropod Borne Dis, C Piqueras 98, Logrono 26006, La Rioja, Spain. | - |
local.uhasselt.international | yes | - |
item.fullcitation | Al-Khafaji, Alaa M.; Bell-Sakyi, Lesley; Fracasso, Gerardo; Luu, Lisa; HEYLEN, Dieter; Matthysen, Erik; Oteo, Jose A. & Palomar, Ana M. (2020) Isolation of Candidatus Rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines. In: Ticks and Tick-Borne Diseases, 11 (6) (Art N° 101511). | - |
item.fulltext | With Fulltext | - |
item.contributor | Al-Khafaji, Alaa M. | - |
item.contributor | Bell-Sakyi, Lesley | - |
item.contributor | Fracasso, Gerardo | - |
item.contributor | Luu, Lisa | - |
item.contributor | HEYLEN, Dieter | - |
item.contributor | Matthysen, Erik | - |
item.contributor | Oteo, Jose A. | - |
item.contributor | Palomar, Ana M. | - |
item.validation | ecoom 2021 | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 1877-959X | - |
crisitem.journal.eissn | 1877-9603 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S1877959X20300893-main.pdf | Published version | 3.08 MB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
5
checked on Oct 6, 2024
Page view(s)
30
checked on Sep 6, 2022
Download(s)
12
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.