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http://hdl.handle.net/1942/3315
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DC Field | Value | Language |
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dc.contributor.author | SYNKOVÁ, Helena | - |
dc.contributor.author | VAN LOVEN, Karen | - |
dc.contributor.author | VALCKE, Roland | - |
dc.date.accessioned | 2007-11-27T14:16:56Z | - |
dc.date.available | 2007-11-27T14:16:56Z | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | PHOTOSYNTHETICA, 33(3-4). p. 595-608 | - |
dc.identifier.issn | 0300-3604 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3315 | - |
dc.description.abstract | The effect of stress (long-term darkening) on the structure and functioning of the photosynthetic apparatus was studied in leaves of non-transformed as well as two types of ipt-transformed tobacco (Nicotiana tabacum cv. Petit Havana SR1) plants, The ipt-gene controlling the biosynthesis of cytokinins (CKs) was coupled to the light-inducible Pssu-promoter of Pisum sativum of to the heat-inducible hsp-promoter of Drosophila melanogaster. Pssu-ipt transgenic grafts with high contents of endogenous CKs retained their chlorophyll (Chi) content during a 15 d dark treatment while the SR1- and heat-treated Phsp 70-ipt seedlings, which did not differ significantly in CKs content, lost up to 60 % of their Chi. The normalised variable fluorescence ratio (F-v/F-m) and oxygen evolution decreased dramatically in the course of continuous dark treatment, indicating a degradation of photosystem. 2 irrespective of the plant type. Changes in the polypeptide composition of thylakoid membranes, as analysed by SDS-PAGE, confirmed this degradation process. Light and electron microscopic observations of leaf sections, and of the ultrastructure of plastids showed changes corresponding to a degradation of the photosynthetic apparatus. | - |
dc.language.iso | en | - |
dc.publisher | INST EXPERIMENTAL BOTANY, ACAD SCI CZECH REPUBLIC | - |
dc.subject.other | carotenoids; chlorophyll content and fluorescence kinetics; chloroplast ultrastructure; cytokinin; dark stress; ipt-gene; photosynthesis | - |
dc.title | Increased content of endogenous cytokinins does riot delay degradation of photosynthetic apparatus in tobacco | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 608 | - |
dc.identifier.issue | 3-4 | - |
dc.identifier.spage | 595 | - |
dc.identifier.volume | 33 | - |
local.format.pages | 14 | - |
dc.description.notes | LIMBURGS UNIV CTR,DEPT SBG,B-3590 DIEPENBEEK,BELGIUM.Synkova, H, ACAD SCI CZECH REPUBL,INST EXPT BOT,KARLOVCE 1A,CZ-16000 PRAGUE 6,CZECH REPUBLIC. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1002/9780470988855.ch13 | - |
dc.identifier.isi | A1997YA11100026 | - |
item.accessRights | Closed Access | - |
item.fullcitation | SYNKOVÁ, Helena; VAN LOVEN, Karen & VALCKE, Roland (1997) Increased content of endogenous cytokinins does riot delay degradation of photosynthetic apparatus in tobacco. In: PHOTOSYNTHETICA, 33(3-4). p. 595-608. | - |
item.contributor | SYNKOVÁ, Helena | - |
item.contributor | VAN LOVEN, Karen | - |
item.contributor | VALCKE, Roland | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Research publications |
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