Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3315
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dc.contributor.authorSYNKOVÁ, Helena-
dc.contributor.authorVAN LOVEN, Karen-
dc.contributor.authorVALCKE, Roland-
dc.date.accessioned2007-11-27T14:16:56Z-
dc.date.available2007-11-27T14:16:56Z-
dc.date.issued1997-
dc.identifier.citationPHOTOSYNTHETICA, 33(3-4). p. 595-608-
dc.identifier.issn0300-3604-
dc.identifier.urihttp://hdl.handle.net/1942/3315-
dc.description.abstractThe effect of stress (long-term darkening) on the structure and functioning of the photosynthetic apparatus was studied in leaves of non-transformed as well as two types of ipt-transformed tobacco (Nicotiana tabacum cv. Petit Havana SR1) plants, The ipt-gene controlling the biosynthesis of cytokinins (CKs) was coupled to the light-inducible Pssu-promoter of Pisum sativum of to the heat-inducible hsp-promoter of Drosophila melanogaster. Pssu-ipt transgenic grafts with high contents of endogenous CKs retained their chlorophyll (Chi) content during a 15 d dark treatment while the SR1- and heat-treated Phsp 70-ipt seedlings, which did not differ significantly in CKs content, lost up to 60 % of their Chi. The normalised variable fluorescence ratio (F-v/F-m) and oxygen evolution decreased dramatically in the course of continuous dark treatment, indicating a degradation of photosystem. 2 irrespective of the plant type. Changes in the polypeptide composition of thylakoid membranes, as analysed by SDS-PAGE, confirmed this degradation process. Light and electron microscopic observations of leaf sections, and of the ultrastructure of plastids showed changes corresponding to a degradation of the photosynthetic apparatus.-
dc.language.isoen-
dc.publisherINST EXPERIMENTAL BOTANY, ACAD SCI CZECH REPUBLIC-
dc.subject.othercarotenoids; chlorophyll content and fluorescence kinetics; chloroplast ultrastructure; cytokinin; dark stress; ipt-gene; photosynthesis-
dc.titleIncreased content of endogenous cytokinins does riot delay degradation of photosynthetic apparatus in tobacco-
dc.typeJournal Contribution-
dc.identifier.epage608-
dc.identifier.issue3-4-
dc.identifier.spage595-
dc.identifier.volume33-
local.format.pages14-
dc.description.notesLIMBURGS UNIV CTR,DEPT SBG,B-3590 DIEPENBEEK,BELGIUM.Synkova, H, ACAD SCI CZECH REPUBL,INST EXPT BOT,KARLOVCE 1A,CZ-16000 PRAGUE 6,CZECH REPUBLIC.-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.bibliographicCitation.oldjcatA1-
dc.identifier.doi10.1002/9780470988855.ch13-
dc.identifier.isiA1997YA11100026-
item.fulltextNo Fulltext-
item.accessRightsClosed Access-
item.contributorVALCKE, Roland-
item.contributorSYNKOVÁ, Helena-
item.contributorVAN LOVEN, Karen-
item.fullcitationSYNKOVÁ, Helena; VAN LOVEN, Karen & VALCKE, Roland (1997) Increased content of endogenous cytokinins does riot delay degradation of photosynthetic apparatus in tobacco. In: PHOTOSYNTHETICA, 33(3-4). p. 595-608.-
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