Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/33177
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dc.contributor.authorLUYTEN, Leen-
dc.contributor.authorDieu, Marc-
dc.contributor.authorDemazy, Catherine-
dc.contributor.authorFransolet, Maude-
dc.contributor.authorNAWROT, Tim-
dc.contributor.authorRenard, Patricia-
dc.contributor.authorDebacq-Chainiaux, Florence-
dc.date.accessioned2021-01-26T12:51:05Z-
dc.date.available2021-01-26T12:51:05Z-
dc.date.issued2020-
dc.date.submitted2021-01-20T09:24:19Z-
dc.identifier.citationPLACENTA, 101 , p. 159 -162-
dc.identifier.urihttp://hdl.handle.net/1942/33177-
dc.description.abstractThe placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.-
dc.description.sponsorshipLeen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence DebacqChainiaux is a Research Associate of the FNRS, Belgium.-
dc.language.isoen-
dc.publisherW B SAUNDERS CO LTD-
dc.subject.otherPlacenta-
dc.subject.otherProteomics-
dc.subject.otherLabel-free nano LC-MS/MS-
dc.titleOptimization of label-free nano LC-MS/MS analysis of the placental proteome-
dc.typeJournal Contribution-
dc.identifier.epage162-
dc.identifier.spage159-
dc.identifier.volume101-
local.format.pages4-
local.bibliographicCitation.jcatA1-
dc.description.notesDebacq-Chainiaux, F (corresponding author), Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium.-
dc.description.notesflorence.chainiaux@unamur.be-
dc.description.otherDebacq-Chainiaux, F (corresponding author), Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium. florence.chainiaux@unamur.be-
local.publisher.place32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1016/j.placenta.2020.09.013-
dc.identifier.isiWOS:000590600700021-
dc.contributor.orcidDieu, Marc/0000-0002-3902-542X-
local.provider.typewosris-
local.uhasselt.uhpubyes-
local.description.affiliation[Luyten, Leen J.; Dieu, Marc; Demazy, Catherine; Fransolet, Maude; Renard, Patricia; Debacq-Chainiaux, Florence] Univ Namur UNamur, Unite Rech Biol Cellulaire URBC, Namur Res Inst Life Sci Narilis, Namur, Belgium.-
local.description.affiliation[Luyten, Leen J.; Nawrot, Tim S.] Hasselt Univ UHasselt, Ctr Environm Sci, Diepenbeek, Belgium.-
local.description.affiliation[Dieu, Marc; Demazy, Catherine; Fransolet, Maude; Renard, Patricia] Univ Namur UNamur, Mass Spectrometry Facil, MaSUN, Namur, Belgium.-
local.description.affiliation[Nawrot, Tim S.] Leuven Univ KULeuven, Dept Publ Hlth & Primary Care, Occupat & Environm Med, Leuven, Belgium.-
local.uhasselt.internationalno-
item.fullcitationLUYTEN, Leen; Dieu, Marc; Demazy, Catherine; Fransolet, Maude; NAWROT, Tim; Renard, Patricia & Debacq-Chainiaux, Florence (2020) Optimization of label-free nano LC-MS/MS analysis of the placental proteome. In: PLACENTA, 101 , p. 159 -162.-
item.validationecoom 2022-
item.contributorLUYTEN, Leen-
item.contributorDieu, Marc-
item.contributorDemazy, Catherine-
item.contributorFransolet, Maude-
item.contributorNAWROT, Tim-
item.contributorRenard, Patricia-
item.contributorDebacq-Chainiaux, Florence-
item.fulltextWith Fulltext-
item.accessRightsRestricted Access-
crisitem.journal.issn0143-4004-
crisitem.journal.eissn1532-3102-
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