Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/33490
Title: Unsupervised Machine Learning-Based Clustering of Nanosized Fluorescent Extracellular Vesicles
Authors: KUYPERS, Soren 
SMISDOM, Nick 
AMELOOT, Marcel 
MICHIELS, Luc 
HENDRIX, Jelle 
HOSSEINKHANI, Baharak 
Pintelon, Isabel
Timmermans, Jean-Pierre
Issue Date: 2021
Publisher: WILEY-V C H VERLAG GMBH
Source: Small, 17 (5) (Art N° 2006786)
Abstract: Extracellular vesicles (EV) are biological nanoparticles that play an important role in cell-to-cell communication. The phenotypic profile of EV populations is a promising reporter of disease, with direct clinical diagnostic relevance. Yet, robust methods for quantifying the biomarker content of EV have been critically lacking, and require a single-particle approach due to their inherent heterogeneous nature. Here, multicolor single-molecule burst analysis microscopy is used to detect multiple biomarkers present on single EV. The authors classify the recorded signals and apply the machine learning-based t-distributed stochastic neighbor embedding algorithm to cluster the resulting multidimensional data. As a proof of principle, the authors use the method to assess both the purity and the inflammatory status of EV, and compare cell culture and plasma-derived EV isolated via different purification methods. This methodology is then applied to identify intercellular adhesion molecule-1 specific EV subgroups released by inflamed endothelial cells, and to prove that apolipoprotein-a1 is an excellent marker to identify the typical lipoprotein contamination in plasma. This methodology can be widely applied on standard confocal microscopes, thereby allowing both standardized quality assessment of patient plasma EV preparations, and diagnostic profiling of multiple EV biomarkers in health and disease.
Notes: Hendrix, J; Hosseinkhani, B (corresponding author), Hasselt Univ, Biomed Res Inst BIOMED, Martelarenlaan 42, B-3500 Hasselt, Belgium.; Hendrix, J (corresponding author), Hasselt Univ, Adv Opt Microscopy Ctr, Dynam Bioimaging Lab, B-3500 Hasselt, Belgium.
Jelle.Hendrix@uhasselt.be; Baharak.Hosseinkhani@uhasselt.be
Other: Hendrix, J; Hosseinkhani, B (corresponding author), Hasselt Univ, Biomed Res Inst BIOMED, Martelarenlaan 42, B-3500 Hasselt, Belgium ; Hasselt Univ, Adv Opt Microscopy Ctr, Dynam Bioimaging Lab, B-3500 Hasselt, Belgium. Jelle.Hendrix@uhasselt.be; Baharak.Hosseinkhani@uhasselt.be
Keywords: burst analysis spectroscopy;extracellular vesicles;machine learning;multidimensional phenotyping
Document URI: http://hdl.handle.net/1942/33490
ISSN: 1613-6810
e-ISSN: 1613-6829
DOI: 10.1002/smll.202006786
ISI #: WOS:000607604800001
Rights: 2021 Wiley-VCH GmbH
Category: A1
Type: Journal Contribution
Validations: ecoom 2022
Appears in Collections:Research publications

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