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http://hdl.handle.net/1942/33890
Title: | Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System | Authors: | De Zitter, Elke Hugelier, Siewert DUWE, Sam Vandenberg, Wim Tebo, Alison G. Van Meervelt, Luc Dedecker, Peter |
Issue Date: | 2021 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | Angewandte Chemie (International edition), 60 (18), p. 10073-10081 | Abstract: | Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure-function relationships. We examined this using fluorescent proteins as easy-to-modify model systems. Starting from a single scaffold, we have developed a range of 27 photochromic fluorescent proteins that cover a broad range of spectroscopic properties, including the determination of 43 crystal structures. Correlation and principal component analysis confirmed the complex relationship between structure and spectroscopy, but also allowed us to identify consistent trends and to relate these to the spatial organization. We find that changes in spectroscopic properties can come about through multiple underlying mechanisms, of which polarity, hydrogen bonding and presence of water molecules are key modulators. We anticipate that our findings and rich structure/spectroscopy dataset can open opportunities for the development and evaluation of new and existing protein engineering methods. | Notes: | Dedecker, P (corresponding author), Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200G Box 2403, B-3001 Leuven, Belgium. peter.dedecker@kuleuven.be |
Other: | Dedecker, P (corresponding author), Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200G Box 2403, B-3001 Leuven, Belgium. peter.dedecker@kuleuven.be | Keywords: | biophysics;fluorescent proteins;photochromism;structure–function relationships | Document URI: | http://hdl.handle.net/1942/33890 | ISSN: | 1433-7851 | e-ISSN: | 1521-3773 | DOI: | 10.1002/anie.202015201 | ISI #: | WOS:000630148800001 | Rights: | 2021 Wiley-VCH GmbH | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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rsGreenMutants.pdf | Peer-reviewed author version | 9.01 MB | Adobe PDF | View/Open |
Angew Chem Int Ed - 2021 - De Zitter - Structure Function Dataset Reveals Environment Effects within a Fluorescent Protein.pdf Restricted Access | Published version | 3 MB | Adobe PDF | View/Open Request a copy |
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