Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/34110
Title: Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer
Authors: MERCKX, Greet 
LO MONACO, Melissa 
LAMBRICHTS, Ivo 
Himmelreich, U
BRONCKAERS, Annelies 
WOLFS, Esther 
Issue Date: 2021
Publisher: SPRINGER
Source: Stem Cell Reviews and Reports, 17(5), p. 1619-1634
Abstract: Background Head and neck cancer (HNC) is one of the most common cancers, associated with a huge mortality and morbidity. In order to improve patient outcomes, more efficient and targeted therapies are essential. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) express tumour homing capacity, which could be exploited to target anti-cancer drug delivery to the tumour region and reduce adverse side-effects. Nevertheless, dental pulp stromal cells (DPSCs), an MSC-like population present in teeth, could offer important clinical benefits because of their easy isolation and superior proliferation compared to BM-MSCs. Therefore, we aimed to elucidate the tumour homing and safe usage of DPSCs to treat HNC.Methods The in vivo survival as well as the effect of intratumourally administered DPSCs on tumour aggressiveness was tested in a HNC xenograft mouse model by using bioluminescence imaging (BLI), (immuno)histology and qRT-PCR. Furthermore, the in vitro and in vivo tumour homing capacity of DPSCs towards a HNC cell line were evaluated by a transwell migration assay and BLI, respectively.Results Intratumourally injected DPSCs survived for at least two weeks in the tumour micro-environment and had no significant influence on tumour morphology, growth, angiogenesis and epithelial-to-mesenchymal transition. In addition, DPSCs migrated towards tumour cells in vitro, which could not be confirmed after their in vivo intravenous, intraperitoneal or peritumoural injection under the tested experimental conditions.Conclusions Our research suggests that intratumourally delivered DPSCs might be used as safe factories for the continuous delivery of anti-cancer center dot drugs in HNC. Nevertheless, further optimization as well as efficacy studies are necessary to understand and improve in vivo tumour homing and determine the optimal experimental set-up of stem cell-based cancer therapies, including dosing and timing.
Keywords: Human dental pulp stromal cells;Head and neck cancer;Tumour homing;Angiogenesis;Epithelial-to-mesenchymal transition;Cell survival;Xcnograft mouse model
Document URI: http://hdl.handle.net/1942/34110
ISSN: 2629-3269
e-ISSN: 2629-3277
DOI: 10.1007/s12015-021-10159-1
ISI #: WOS:000637469300001
Rights: The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021
Category: A1
Type: Journal Contribution
Validations: ecoom 2022
Appears in Collections:Research publications

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