Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/34110
Title: | Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer | Authors: | MERCKX, Greet LO MONACO, Melissa LAMBRICHTS, Ivo Himmelreich, U BRONCKAERS, Annelies WOLFS, Esther |
Issue Date: | 2021 | Publisher: | SPRINGER | Source: | Stem Cell Reviews and Reports, 17(5), p. 1619-1634 | Abstract: | Background Head and neck cancer (HNC) is one of the most common cancers, associated with a huge mortality and morbidity. In order to improve patient outcomes, more efficient and targeted therapies are essential. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) express tumour homing capacity, which could be exploited to target anti-cancer drug delivery to the tumour region and reduce adverse side-effects. Nevertheless, dental pulp stromal cells (DPSCs), an MSC-like population present in teeth, could offer important clinical benefits because of their easy isolation and superior proliferation compared to BM-MSCs. Therefore, we aimed to elucidate the tumour homing and safe usage of DPSCs to treat HNC.Methods The in vivo survival as well as the effect of intratumourally administered DPSCs on tumour aggressiveness was tested in a HNC xenograft mouse model by using bioluminescence imaging (BLI), (immuno)histology and qRT-PCR. Furthermore, the in vitro and in vivo tumour homing capacity of DPSCs towards a HNC cell line were evaluated by a transwell migration assay and BLI, respectively.Results Intratumourally injected DPSCs survived for at least two weeks in the tumour micro-environment and had no significant influence on tumour morphology, growth, angiogenesis and epithelial-to-mesenchymal transition. In addition, DPSCs migrated towards tumour cells in vitro, which could not be confirmed after their in vivo intravenous, intraperitoneal or peritumoural injection under the tested experimental conditions.Conclusions Our research suggests that intratumourally delivered DPSCs might be used as safe factories for the continuous delivery of anti-cancer center dot drugs in HNC. Nevertheless, further optimization as well as efficacy studies are necessary to understand and improve in vivo tumour homing and determine the optimal experimental set-up of stem cell-based cancer therapies, including dosing and timing. | Keywords: | Human dental pulp stromal cells;Head and neck cancer;Tumour homing;Angiogenesis;Epithelial-to-mesenchymal transition;Cell survival;Xcnograft mouse model | Document URI: | http://hdl.handle.net/1942/34110 | ISSN: | 2629-3269 | e-ISSN: | 2629-3277 | DOI: | 10.1007/s12015-021-10159-1 | ISI #: | WOS:000637469300001 | Rights: | The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
s12015-021-10159-1.pdf Restricted Access | Published version | 10.84 MB | Adobe PDF | View/Open Request a copy |
WEB OF SCIENCETM
Citations
3
checked on May 10, 2024
Page view(s)
68
checked on Aug 31, 2022
Download(s)
8
checked on Aug 31, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.