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http://hdl.handle.net/1942/34122
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DC Field | Value | Language |
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dc.contributor.author | BIJNENS, Karolien | - |
dc.contributor.author | JAENEN, Vincent | - |
dc.contributor.author | WOUTERS, Annelies | - |
dc.contributor.author | LEYNEN, Nathalie | - |
dc.contributor.author | PIROTTE, Nicky | - |
dc.contributor.author | ARTOIS, Tom | - |
dc.contributor.author | SMEETS, Karen | - |
dc.date.accessioned | 2021-05-28T08:04:33Z | - |
dc.date.available | 2021-05-28T08:04:33Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-05-27T07:28:34Z | - |
dc.identifier.citation | Biomolecules, 11 (5) (Art N° 714) | - |
dc.identifier.uri | http://hdl.handle.net/1942/34122 | - |
dc.description.abstract | A strict coordination between pro- and antioxidative molecules is needed for normal animal physiology, although their exact function and dynamics during regeneration and development remains largely unknown. Via in vivo imaging, we were able to locate and discriminate between reactive oxygen species (ROS) in real-time during different physiological stages of the highly regenerative planarian Schmidtea mediterranea. All ROS signals were strong enough to overcome the detected autofluorescence. Combined with an in situ characterisation and quantification of the transcription of several antioxidant genes, our data showed that the planarian gut and epidermis have a well-equipped redox system. Pharmacological inhibition or RNA interference of either side of the redox balance resulted in alterations in the regeneration process, characterised by decreased blastema sizes and delayed neurodevelopment, thereby affecting tails more than heads. Focusing on glutathione, a central component in the redox balance, we found that it is highly present in planarians and that a significant reduction in glutathione content led to regenerative failure with tissue lesions, characterised by underlying stem cell alterations. This exploratory study indicates that ROS and antioxidants are tightly intertwined and should be studied as a whole to fully comprehend the function of the redox balance in animal physiology. | - |
dc.description.sponsorship | His work was supported by the Research Foundation Flanders (FWO) (G.0B83.17N, N1522719 and 1522015N and the Bijzonder OnderzoeksFonds of Hasselt University (BOF16NI03). The research leading to results presented in this publication was carried out with infrastructure funded by The European Marine Biological Resource Centre (EMBRC) Belgium—FWO project GOH3817N and I001219N. The authors thank Natascha Steffanie and Ria Vanderspikken for their skilful technical assistance. | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.rights | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
dc.subject.other | planarians | - |
dc.subject.other | wound healing and tissue regeneration | - |
dc.subject.other | stem cells | - |
dc.subject.other | redox molecules | - |
dc.subject.other | reactive oxygen species | - |
dc.subject.other | antioxidants | - |
dc.title | A Spatiotemporal Characterisation of Redox Molecules in Planarians, with a Focus on the Role of Glutathione during Regeneration | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 5 | - |
dc.identifier.volume | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 714 | - |
dc.identifier.doi | 10.3390/biom11050714 | - |
dc.identifier.pmid | 34064618 | - |
dc.identifier.isi | 000653426600001 | - |
dc.identifier.eissn | 2218-273X | - |
local.provider.type | CrossRef | - |
local.uhasselt.uhpub | yes | - |
local.uhasselt.international | no | - |
item.contributor | BIJNENS, Karolien | - |
item.contributor | JAENEN, Vincent | - |
item.contributor | WOUTERS, Annelies | - |
item.contributor | LEYNEN, Nathalie | - |
item.contributor | PIROTTE, Nicky | - |
item.contributor | ARTOIS, Tom | - |
item.contributor | SMEETS, Karen | - |
item.validation | ecoom 2022 | - |
item.fullcitation | BIJNENS, Karolien; JAENEN, Vincent; WOUTERS, Annelies; LEYNEN, Nathalie; PIROTTE, Nicky; ARTOIS, Tom & SMEETS, Karen (2021) A Spatiotemporal Characterisation of Redox Molecules in Planarians, with a Focus on the Role of Glutathione during Regeneration. In: Biomolecules, 11 (5) (Art N° 714). | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.eissn | 2218-273X | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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biomolecules-11-00714-v2.pdf | Published version | 8.12 MB | Adobe PDF | View/Open |
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