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Title: | Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior | Authors: | EzEldeen, Mostafa BurakToprakhisar Murgia, Denise SMISDOM, Nick Deschaume, Olivier Bartic, Carmen Van Oosterwyck, Hans Pereira, Rafaela Vaz Sousa Opdenakkee, Ghislain Jacobs, Reinhilde BRONCKAERS, Annelies LAMBRICHTS, Ivo Patterson, Jennifer |
Issue Date: | 2021 | Publisher: | NATURE RESEARCH | Source: | Scientific reports, 11 (1) (Art N° 5687) | Abstract: | Tailored hydrogels mimicking the native extracellular environment could help overcome the high variability in outcomes within regenerative endodontics. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the microstructural properties of fibrin and self-assembling peptide (SAP) hydrogels. A further goal was to assess the influence of the microstructural differences between the hydrogels on the in vitro behavior of human dental pulp stem cells (hDPSCs). Structural and mechanical characterization of the hydrogels with and without COAM was performed by atomic force microscopy and scanning electron microscopy to characterize their microstructure (roughness and fiber length, diameter, straightness, and alignment) and by nanoindentation to measure their stiffness (elastic modulus). Then, hDPSCs were encapsulated in hydrogels with and without COAM. Cell viability and circularity were determined using confocal microscopy, and proliferation was determined using DNA quantification. Inclusion of COAM did not alter the microstructure of the fibrin hydrogels at the fiber level while affecting the SAP hydrogel microstructure (homogeneity), leading to fiber aggregation. The stiffness of the SAP hydrogels was sevenfold higher than the fibrin hydrogels. The viability and attachment of hDPSCs were significantly higher in fibrin hydrogels than in SAP hydrogels. The DNA content was significantly affected by the hydrogel type and the presence of COAM. The microstructural stability after COAM inclusion and the favorable hDPSCs' response observed in fibrin hydrogels suggest this system as a promising carrier for COAM and application in endodontic regeneration. | Notes: | EzEldeen, M (corresponding author), Katholieke Univ Leuven, Fac Med, Dept Imaging & Pathol, OMFS IMPATH Res Grp, Kapucijnenvoer 33, B-3000 Leuven, Belgium.; EzEldeen, M (corresponding author), Univ Hosp Leuven, Oral & Maxillofacial Surg, Kapucijnenvoer 33, B-3000 Leuven, Belgium.; EzEldeen, M (corresponding author), Katholieke Univ Leuven, Dept Oral Hlth Sci, Kapucijnenvoer 33, B-3000 Leuven, Belgium.; EzEldeen, M (corresponding author), Univ Hosp Leuven, Paediat Dent & Special Dent Care, Kapucijnenvoer 33, B-3000 Leuven, Belgium. mostafa.ezeldeen@kuleuven.be |
Other: | EzEldeen, M (corresponding author), Katholieke Univ Leuven, Fac Med, Dept Imaging & Pathol, OMFS IMPATH Res Grp, Kapucijnenvoer 33, B-3000 Leuven, Belgium ; Univ Hosp Leuven, Oral & Maxillofacial Surg, Kapucijnenvoer 33, B-3000 Leuven, Belgium ; Katholieke Univ Leuven, Dept Oral Hlth Sci, Kapucijnenvoer 33, B-3000 Leuven, Belgium ; Univ Hosp Leuven, Paediat Dent & Special Dent Care, Kapucijnenvoer 33, B-3000 Leuven, Belgium. mostafa.ezeldeen@kuleuven.be | Keywords: | Adolescent;Amylose;Cell Proliferation;Cell Survival;Chlorides;DNA;Dental Pulp;Female;Fibrin;Humans;Hydrogels;Male;Microscopy, Atomic Force;Oxidation-Reduction;Peptides;Stem Cells;Young Adult | Document URI: | http://hdl.handle.net/1942/34171 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/s41598-021-84405-4 | ISI #: | WOS:000629620800015 | Rights: | The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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s41598-021-84405-4.pdf | Published version | 3.79 MB | Adobe PDF | View/Open |
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