Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/34183
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dc.contributor.authorVitale, Maria Rosaria-
dc.contributor.authorZoeller, Johanna Eva Maria-
dc.contributor.authorJansch, Charline-
dc.contributor.authorJanz, Anna-
dc.contributor.authorEdenhofer, Frank-
dc.contributor.authorKlopocki, Eva-
dc.contributor.authorvan den Hove, Daniel-
dc.contributor.authorVANMIERLO, Tim-
dc.contributor.authorRivero, Olga-
dc.contributor.authorKasri, Nael Nadif-
dc.contributor.authorZiegler, Georg Christoph-
dc.contributor.authorLesch, Klaus-Peter-
dc.date.accessioned2021-06-01T18:37:43Z-
dc.date.available2021-06-01T18:37:43Z-
dc.date.issued2021-
dc.date.submitted2021-04-22T08:45:30Z-
dc.identifier.citationStem Cell Research, 51 (Art N° 102169)-
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/1942/34183-
dc.description.abstractFibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.-
dc.description.sponsorshipThis work was supported by ERA-NET NEURON under Grant No. 01EW1902 (DECODE!), the Horizon 2020 Research and Innovation Programme under Grant No. 728018 (Eat2beNICE) andthe 5-100 Russian Academic Excellence Project. This publication was supported by the Open Access Publication Fund of the University of Wuerzburg. G.C.Z is supported by a grant from the DFG (Project No. 413657723 Clinician Scientist-Program UNION CVD). We thank J. Merk for excellent technical assistance and E. Siqueira, R. Riemens, E. Yildirim and F. Benz for excellent contribution to the technical processes.-
dc.language.isoen-
dc.publisherELSEVIER-
dc.rights2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)-
dc.subject.otherCRISPR-Cas Systems-
dc.subject.otherCadherins-
dc.subject.otherFemale-
dc.subject.otherHeterozygote-
dc.subject.otherHumans-
dc.subject.otherMiddle Aged-
dc.subject.otherInduced Pluripotent Stem Cells-
dc.subject.otherNeurodevelopmental Disorders-
dc.titleGeneration of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9-
dc.typeJournal Contribution-
dc.identifier.volume51-
local.format.pages5-
local.bibliographicCitation.jcatA1-
dc.description.notesVitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany.-
dc.description.notesVitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de-
dc.description.otherVitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. Vitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de-
local.publisher.placeRADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr102169-
dc.identifier.doi10.1016/j.scr.2021.102169-
dc.identifier.pmid33486346-
dc.identifier.isiWOS:000629039100019-
dc.contributor.orcidKasri, Nael Nadif/0000-0002-7448-9289; Ziegler, Georg-
dc.contributor.orcidC/0000-0001-9411-3169; Rivero, Olga/0000-0002-2664-4053-
dc.identifier.eissn1876-7753-
local.provider.typewosris-
local.uhasselt.uhpubyes-
local.description.affiliation[Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline; van den Hove, Daniel; Rivero, Olga; Ziegler, Georg Christoph; Lesch, Klaus-Peter] Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany.-
local.description.affiliation[Vitale, Maria Rosaria; Lesch, Klaus-Peter] IM Sechenov First Moscow State Med Univ, Inst Mol Med, Lab Psychiat Neurobiol, Moscow, Russia.-
local.description.affiliation[Zoeller, Johanna Eva Maria; van den Hove, Daniel; Vanmierlo, Tim; Lesch, Klaus-Peter] Maastricht Univ, Sch Mental Hlth & Neurosci MHeNs, Dept Psychiat & Neuropsychol, Maastricht, Netherlands.-
local.description.affiliation[Janz, Anna] Univ Hosp Wurzburg, Comprehens Heart Failure Ctr CHFC, Wurzburg, Germany.-
local.description.affiliation[Janz, Anna] Univ Hosp Wurzburg, Dept Internal Med 1, Wurzburg, Germany.-
local.description.affiliation[Edenhofer, Frank] Leopold Franzens Univ Innsbruck, Inst Mol Biol, Dept Genom Stem Cell Biol & Regenerat Med, Innsbruck, Austria.-
local.description.affiliation[Edenhofer, Frank] Leopold Franzens Univ Innsbruck, CMBI, Innsbruck, Austria.-
local.description.affiliation[Klopocki, Eva] Univ Wurzburg, Bioctr, Inst Human Genet, Wurzburg, Germany.-
local.description.affiliation[Vanmierlo, Tim] Hasselt Univ, Biomed Res Inst, Neuroimmune Connect & Repair Lab, Hasselt, Belgium.-
local.description.affiliation[Kasri, Nael Nadif] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, Dept Cognit Neurosci, Med Ctr, Nijmegen, Netherlands.-
local.description.affiliation[Kasri, Nael Nadif] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, Dept Human Genet, Med Ctr, Nijmegen, Netherlands.-
local.description.affiliation[Ziegler, Georg Christoph] Univ Hosp Wurzburg, Ctr Mental Hlth, Dept Psychiat Psychosomat & Psychotherapy, Wurzburg, Germany.-
local.uhasselt.internationalyes-
item.contributorVitale, Maria Rosaria-
item.contributorZoeller, Johanna Eva Maria-
item.contributorJansch, Charline-
item.contributorJanz, Anna-
item.contributorEdenhofer, Frank-
item.contributorKlopocki, Eva-
item.contributorvan den Hove, Daniel-
item.contributorVANMIERLO, Tim-
item.contributorRivero, Olga-
item.contributorKasri, Nael Nadif-
item.contributorZiegler, Georg Christoph-
item.contributorLesch, Klaus-Peter-
item.fullcitationVitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline; Janz, Anna; Edenhofer, Frank; Klopocki, Eva; van den Hove, Daniel; VANMIERLO, Tim; Rivero, Olga; Kasri, Nael Nadif; Ziegler, Georg Christoph & Lesch, Klaus-Peter (2021) Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. In: Stem Cell Research, 51 (Art N° 102169).-
item.accessRightsOpen Access-
item.fulltextWith Fulltext-
item.validationecoom 2022-
crisitem.journal.issn1873-5061-
crisitem.journal.eissn1876-7753-
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