Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/34183
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Vitale, Maria Rosaria | - |
dc.contributor.author | Zoeller, Johanna Eva Maria | - |
dc.contributor.author | Jansch, Charline | - |
dc.contributor.author | Janz, Anna | - |
dc.contributor.author | Edenhofer, Frank | - |
dc.contributor.author | Klopocki, Eva | - |
dc.contributor.author | van den Hove, Daniel | - |
dc.contributor.author | VANMIERLO, Tim | - |
dc.contributor.author | Rivero, Olga | - |
dc.contributor.author | Kasri, Nael Nadif | - |
dc.contributor.author | Ziegler, Georg Christoph | - |
dc.contributor.author | Lesch, Klaus-Peter | - |
dc.date.accessioned | 2021-06-01T18:37:43Z | - |
dc.date.available | 2021-06-01T18:37:43Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-04-22T08:45:30Z | - |
dc.identifier.citation | Stem Cell Research, 51 (Art N° 102169) | - |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.uri | http://hdl.handle.net/1942/34183 | - |
dc.description.abstract | Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype. | - |
dc.description.sponsorship | This work was supported by ERA-NET NEURON under Grant No. 01EW1902 (DECODE!), the Horizon 2020 Research and Innovation Programme under Grant No. 728018 (Eat2beNICE) andthe 5-100 Russian Academic Excellence Project. This publication was supported by the Open Access Publication Fund of the University of Wuerzburg. G.C.Z is supported by a grant from the DFG (Project No. 413657723 Clinician Scientist-Program UNION CVD). We thank J. Merk for excellent technical assistance and E. Siqueira, R. Riemens, E. Yildirim and F. Benz for excellent contribution to the technical processes. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.rights | 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | - |
dc.subject.other | CRISPR-Cas Systems | - |
dc.subject.other | Cadherins | - |
dc.subject.other | Female | - |
dc.subject.other | Heterozygote | - |
dc.subject.other | Humans | - |
dc.subject.other | Middle Aged | - |
dc.subject.other | Induced Pluripotent Stem Cells | - |
dc.subject.other | Neurodevelopmental Disorders | - |
dc.title | Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9 | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 51 | - |
local.format.pages | 5 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Vitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. | - |
dc.description.notes | Vitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de | - |
dc.description.other | Vitale, MR; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. Vitale_M@ukw.de; kplesch@mail.uni-wuerzburg.de | - |
local.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 102169 | - |
dc.identifier.doi | 10.1016/j.scr.2021.102169 | - |
dc.identifier.pmid | 33486346 | - |
dc.identifier.isi | WOS:000629039100019 | - |
dc.contributor.orcid | Kasri, Nael Nadif/0000-0002-7448-9289; Ziegler, Georg | - |
dc.contributor.orcid | C/0000-0001-9411-3169; Rivero, Olga/0000-0002-2664-4053 | - |
dc.identifier.eissn | 1876-7753 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline; van den Hove, Daniel; Rivero, Olga; Ziegler, Georg Christoph; Lesch, Klaus-Peter] Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany. | - |
local.description.affiliation | [Vitale, Maria Rosaria; Lesch, Klaus-Peter] IM Sechenov First Moscow State Med Univ, Inst Mol Med, Lab Psychiat Neurobiol, Moscow, Russia. | - |
local.description.affiliation | [Zoeller, Johanna Eva Maria; van den Hove, Daniel; Vanmierlo, Tim; Lesch, Klaus-Peter] Maastricht Univ, Sch Mental Hlth & Neurosci MHeNs, Dept Psychiat & Neuropsychol, Maastricht, Netherlands. | - |
local.description.affiliation | [Janz, Anna] Univ Hosp Wurzburg, Comprehens Heart Failure Ctr CHFC, Wurzburg, Germany. | - |
local.description.affiliation | [Janz, Anna] Univ Hosp Wurzburg, Dept Internal Med 1, Wurzburg, Germany. | - |
local.description.affiliation | [Edenhofer, Frank] Leopold Franzens Univ Innsbruck, Inst Mol Biol, Dept Genom Stem Cell Biol & Regenerat Med, Innsbruck, Austria. | - |
local.description.affiliation | [Edenhofer, Frank] Leopold Franzens Univ Innsbruck, CMBI, Innsbruck, Austria. | - |
local.description.affiliation | [Klopocki, Eva] Univ Wurzburg, Bioctr, Inst Human Genet, Wurzburg, Germany. | - |
local.description.affiliation | [Vanmierlo, Tim] Hasselt Univ, Biomed Res Inst, Neuroimmune Connect & Repair Lab, Hasselt, Belgium. | - |
local.description.affiliation | [Kasri, Nael Nadif] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, Dept Cognit Neurosci, Med Ctr, Nijmegen, Netherlands. | - |
local.description.affiliation | [Kasri, Nael Nadif] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, Dept Human Genet, Med Ctr, Nijmegen, Netherlands. | - |
local.description.affiliation | [Ziegler, Georg Christoph] Univ Hosp Wurzburg, Ctr Mental Hlth, Dept Psychiat Psychosomat & Psychotherapy, Wurzburg, Germany. | - |
local.uhasselt.international | yes | - |
item.contributor | Vitale, Maria Rosaria | - |
item.contributor | Zoeller, Johanna Eva Maria | - |
item.contributor | Jansch, Charline | - |
item.contributor | Janz, Anna | - |
item.contributor | Edenhofer, Frank | - |
item.contributor | Klopocki, Eva | - |
item.contributor | van den Hove, Daniel | - |
item.contributor | VANMIERLO, Tim | - |
item.contributor | Rivero, Olga | - |
item.contributor | Kasri, Nael Nadif | - |
item.contributor | Ziegler, Georg Christoph | - |
item.contributor | Lesch, Klaus-Peter | - |
item.fullcitation | Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline; Janz, Anna; Edenhofer, Frank; Klopocki, Eva; van den Hove, Daniel; VANMIERLO, Tim; Rivero, Olga; Kasri, Nael Nadif; Ziegler, Georg Christoph & Lesch, Klaus-Peter (2021) Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. In: Stem Cell Research, 51 (Art N° 102169). | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2022 | - |
crisitem.journal.issn | 1873-5061 | - |
crisitem.journal.eissn | 1876-7753 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s20-S1873506121000155-main.pdf | Published version | 2.29 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.