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http://hdl.handle.net/1942/34353
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DC Field | Value | Language |
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dc.contributor.author | Abouelasrar Salama, Sara | - |
dc.contributor.author | DE BONDT, Mirre | - |
dc.contributor.author | De Buck, Mieke | - |
dc.contributor.author | Berghmans, Nele | - |
dc.contributor.author | PROOST, Paul | - |
dc.contributor.author | Oliveira, Vivian Louise Soares | - |
dc.contributor.author | Amaral, Flavio A | - |
dc.contributor.author | GOUWY, Mieke | - |
dc.contributor.author | Van Damme, Jo | - |
dc.contributor.author | Struyf, Sofie | - |
dc.date.accessioned | 2021-06-25T10:25:38Z | - |
dc.date.available | 2021-06-25T10:25:38Z | - |
dc.date.issued | 2020 | - |
dc.date.submitted | 2021-06-18T06:43:41Z | - |
dc.identifier.citation | Frontiers in Immunology, 11 (843) (Art N° 843) | - |
dc.identifier.uri | http://hdl.handle.net/1942/34353 | - |
dc.description.abstract | Infection, sterile injury, and chronic inflammation trigger the acute phase response in order to re-establish homeostasis. This response includes production of positive acute phase proteins in the liver, such as members of the serum amyloid A (SAA) family. In humans the major acute phase SAAs comprise a group of closely related variants of SAA1 and SAA2. SAA1 was proven to be chemotactic for several leukocyte subtypes through activation of the G protein-coupled receptor FPRL1/FPR2. Several other biological activities of SAA1, such as cytokine induction, reported to be mediated via TLRs, have been debated recently. Especially commercial SAA1, recombinantly produced in Escherichia coli, was found to be contaminated with bacterial products confounding biological assays performed with this rSAA1. We purified rSAA1 by RP-HPLC to homogeneity, removing contaminants such as lipopolysaccharides, lipoproteins and formylated peptides, and re-assessed several biological activities attributed to SAA1 (chemotaxis, cytokine induction, MMP-9 release, ROS generation, and macrophage differentiation). The homogeneous rSAA1 (hrSAA1) lacked most cell-activating properties, but its leukocyte-recruiting capacity in vivo and it's in vitro synergy with other leukocyte attractants remained preserved. Furthermore, hrSAA1 maintained the ability to promote monocyte survival. This indicates that pure hrSAA1 retains its potential to activate FPR2, whereas TLR-mediated effects seem to be related to traces of bacterial TLR ligands in the E. coli-produced human rSAA1. | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.rights | Copyright © 2020 Abouelasrar Salama, De Bondt, De Buck, Berghmans, Proost, Oliveira, Amaral, Gouwy, Van Damme and Struyf. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | - |
dc.subject.other | FPR2 | - |
dc.subject.other | MMP-9 | - |
dc.subject.other | ROS | - |
dc.subject.other | SAA | - |
dc.subject.other | chemokines | - |
dc.subject.other | chemotaxis | - |
dc.subject.other | macrophages | - |
dc.subject.other | neutrophils | - |
dc.subject.other | Blood Donors | - |
dc.subject.other | Cell Differentiation | - |
dc.subject.other | Cell Survival | - |
dc.subject.other | Chemotaxis | - |
dc.subject.other | Cytokines | - |
dc.subject.other | Escherichia coli | - |
dc.subject.other | HEK293 Cells | - |
dc.subject.other | Humans | - |
dc.subject.other | Leukocytes | - |
dc.subject.other | Macrophages | - |
dc.subject.other | Matrix Metalloproteinase 9 | - |
dc.subject.other | Monocytes | - |
dc.subject.other | Reactive Oxygen Species | - |
dc.subject.other | Receptors, Formyl Peptide | - |
dc.subject.other | Receptors, Lipoxin | - |
dc.subject.other | Recombinant Proteins | - |
dc.subject.other | Serum Amyloid A Protein | - |
dc.subject.other | Signal Transduction | - |
dc.subject.other | Toll-Like Receptors | - |
dc.subject.other | Transfection | - |
dc.title | Serum Amyloid A1 (SAA1) Revisited: Restricted Leukocyte-Activating Properties of Homogeneous SAA1 | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 843 | - |
dc.identifier.volume | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 843 | - |
dc.identifier.doi | 10.3389/fimmu.2020.00843 | - |
dc.identifier.pmid | 32477346 | - |
dc.identifier.isi | WOS:000537803900001 | - |
local.provider.type | PubMed | - |
local.uhasselt.uhpub | yes | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.contributor | Abouelasrar Salama, Sara | - |
item.contributor | DE BONDT, Mirre | - |
item.contributor | De Buck, Mieke | - |
item.contributor | Berghmans, Nele | - |
item.contributor | PROOST, Paul | - |
item.contributor | Oliveira, Vivian Louise Soares | - |
item.contributor | Amaral, Flavio A | - |
item.contributor | GOUWY, Mieke | - |
item.contributor | Van Damme, Jo | - |
item.contributor | Struyf, Sofie | - |
item.fullcitation | Abouelasrar Salama, Sara; DE BONDT, Mirre; De Buck, Mieke; Berghmans, Nele; PROOST, Paul; Oliveira, Vivian Louise Soares; Amaral, Flavio A; GOUWY, Mieke; Van Damme, Jo & Struyf, Sofie (2020) Serum Amyloid A1 (SAA1) Revisited: Restricted Leukocyte-Activating Properties of Homogeneous SAA1. In: Frontiers in Immunology, 11 (843) (Art N° 843). | - |
crisitem.journal.issn | 1664-3224 | - |
crisitem.journal.eissn | 1664-3224 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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fimmu-11-00843.pdf | Published version | 3.55 MB | Adobe PDF | View/Open |
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