Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/34723
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dc.contributor.authorDu Cheyne, Charis-
dc.contributor.authorChen, Yao-
dc.contributor.authorDe Craene, Jurgen-
dc.contributor.authorTHAS, Olivier-
dc.contributor.authorDe Spiegelaere, Ward-
dc.date.accessioned2021-08-28T18:57:07Z-
dc.date.available2021-08-28T18:57:07Z-
dc.date.issued2021-
dc.date.submitted2021-08-28T18:34:34Z-
dc.identifier.citationAnalytical biochemistry (Print), 626 (Art N° 114217)-
dc.identifier.urihttp://hdl.handle.net/1942/34723-
dc.description.abstractAccurate tools to measure RNA integrity are essential to obtain reliable gene expression data. The reverse transcription quantitative PCR (RT-qPCR) based 3':5 ' assay permits a direct determination of messenger RNA (mRNA) integrity. However, the use of standard curves and the possible effect of PCR inhibitors make this method cumbersome and prone to variation, especially in small samples. Here we developed a triplex digital PCR (dPCR) 3':5' assay for assessing RNA integrity in equine samples as rapid and simple alternative to RT-qPCR. This dPCR assay not only provides a straight forward analysis of the mRNA integrity, but also of its quantity.-
dc.description.sponsorshipCharis Du Cheyne holds a PhD grant strategic basic research of the (Research Foundation - Flanders FWO-SB 1S22820N). The research was also supported by a research grant (FWO 1508419N).-
dc.language.isoen-
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE-
dc.rights2021 Elsevier Inc. All rights reserved.-
dc.subject.otherDigital PCR-
dc.subject.otherdPCR-
dc.subject.otherRNA integrity-
dc.subject.otherRNA quality-
dc.titleDevelopment of a 3':5' digital PCR assay to determine horse mRNA integrity-
dc.typeJournal Contribution-
dc.identifier.volume626-
local.format.pages3-
local.bibliographicCitation.jcatA1-
dc.description.notesDe Spiegelaere, W (corresponding author), Univ Ghent, Fac Vet Med, Dept Morphol, Salisburylaan 133, B-9820 Merelbeke, Belgium.-
dc.description.notesward.despiegelaere@ugent.be-
local.publisher.place525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr114217-
dc.identifier.doi10.1016/j.ab.2021.114217-
dc.identifier.isiWOS:000663454300002-
dc.contributor.orcidDe Craene, Jurgen/0000-0002-9009-4893; Du Cheyne,-
dc.contributor.orcidCharis/0000-0002-8675-8052; De Spiegelaere, Ward/0000-0003-2097-8439-
local.provider.typewosris-
local.uhasselt.uhpubyes-
local.description.affiliation[Du Cheyne, Charis; Chen, Yao; De Craene, Jurgen; De Spiegelaere, Ward] Univ Ghent, Fac Vet Med, Dept Morphol, Salisburylaan 133, B-9820 Merelbeke, Belgium.-
local.description.affiliation[Chen, Yao; Thas, Olivier] Univ Ghent, Dept Data Anal & Math Modelling, B-9000 Ghent, Belgium.-
local.description.affiliation[Chen, Yao; Thas, Olivier; De Spiegelaere, Ward] Univ Ghent, Digital PCR Consortium, B-9000 Ghent, Belgium.-
local.description.affiliation[Thas, Olivier] Hasselt Univ, I BioStat, Data Sci Inst, B-3500 Hasselt, Belgium.-
local.description.affiliation[Thas, Olivier] Univ Wollongong, Natl Inst Appl Stat Res Australia NIASRA, Wollongong, NSW 2500, Australia.-
local.uhasselt.internationalyes-
item.fullcitationDu Cheyne, Charis; Chen, Yao; De Craene, Jurgen; THAS, Olivier & De Spiegelaere, Ward (2021) Development of a 3':5' digital PCR assay to determine horse mRNA integrity. In: Analytical biochemistry (Print), 626 (Art N° 114217).-
item.fulltextWith Fulltext-
item.contributorDu Cheyne, Charis-
item.contributorChen, Yao-
item.contributorDe Craene, Jurgen-
item.contributorTHAS, Olivier-
item.contributorDe Spiegelaere, Ward-
item.validationecoom 2022-
item.accessRightsRestricted Access-
crisitem.journal.issn0003-2697-
crisitem.journal.eissn1096-0309-
Appears in Collections:Research publications
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