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http://hdl.handle.net/1942/34794
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DC Field | Value | Language |
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dc.contributor.author | Boon, Maarten | - |
dc.contributor.author | Ceyssens, Pieter-Jan | - |
dc.contributor.author | Voet, Marleen | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Andreeva, Julia | - |
dc.contributor.author | Ghilarov, Dmitry | - |
dc.contributor.author | Severinov, Konstantin | - |
dc.contributor.author | Lavigne, Rob | - |
dc.contributor.author | Wagemans, Jeroen | - |
dc.contributor.author | De Smet, Jeroen | - |
dc.date.accessioned | 2021-09-02T15:27:12Z | - |
dc.date.available | 2021-09-02T15:27:12Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-09-02T10:08:09Z | - |
dc.identifier.citation | CELL REPORTS, 36 (8) (Art N° 109567) | - |
dc.identifier.uri | http://hdl.handle.net/1942/34794 | - |
dc.description.abstract | The bacterial DNA gyrase complex (GyrA/GyrB) plays a crucial role during DNA replication and serves as a target for multiple antibiotics, including the fluoroquinolones. Despite it being a valuable antibiotics target, resistance emergence by pathogens including Pseudomonas aeruginosa are proving problematic. Here, we describe Igy, a peptide inhibitor of gyrase, encoded by Pseudomonas bacteriophage LUZ24 and other members of the Bruynoghevirus genus. Igy (5.6 kDa) inhibits in vitro gyrase activity and interacts with the P. aeruginosa GyrB subunit, possibly by DNA mimicry, as indicated by a de novo model of the peptide and mutagenesis. In vivo, overproduction of Igy blocks DNA replication and leads to cell death also in fluoroquinolone-resistant bacterial isolates. These data highlight the potential of discovering phage-inspired leads for antibiotics development, supported by co-evolution, as Igy may serve as a scaffold for small molecule mimicry to target the DNA gyrase complex, without cross-resistance to existing molecules. | - |
dc.description.sponsorship | "Fonds Wetenschappelijk Onderzoek'' (FWO) [G.0323.09]; KULeuven project GOA "Phage Biosystems.'' | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.title | The bacteriophage LUZ24 “Igy” peptide inhibits the Pseudomonas DNA gyrase | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 8 | - |
dc.identifier.volume | 36 | - |
local.format.pages | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Lavigne, R (corresponding author), Katholieke Univ Leuven, Lab Gene Technol, Dept Biosyst, B-3001 Leuven, Belgium. | - |
dc.description.notes | rob.lavigne@kuleuven.be | - |
local.publisher.place | 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 109567 | - |
dc.identifier.doi | 10.1016/j.celrep.2021.109567 | - |
dc.identifier.isi | WOS:000688508300009 | - |
dc.contributor.orcid | Ceyssens, Pieter-Jan/0000-0001-7735-8316; Lavigne, | - |
dc.contributor.orcid | Rob/0000-0001-7377-1314; Andreeva, Julia/0000-0002-1206-8606 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [De Smet, Jeroen; Wagemans, Jeroen; Boon, Maarten; Ceyssens, Pieter-Jan; Voet, Marleen; Lavigne, Rob] Katholieke Univ Leuven, Lab Gene Technol, Dept Biosyst, B-3001 Leuven, Belgium. | - |
local.description.affiliation | [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnatl Univ Limburg, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Andreeva, Julia; Ghilarov, Dmitry; Severinov, Konstantin] Skolkovo Inst Sci & Technol, Ctr Life Sci, Moscow 143026, Russia. | - |
local.description.affiliation | [Severinov, Konstantin] Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 USA. | - |
local.description.affiliation | [De Smet, Jeroen] Katholieke Univ Leuven, Dept Microbial & Mol Syst, Res Grp Insect Prod & Proc, B-2240 Geel, Belgium. | - |
local.description.affiliation | [Ceyssens, Pieter-Jan] Sciensano, Div Bacterial Dis, B-1050 Brussels, Belgium. | - |
local.description.affiliation | [Ghilarov, Dmitry] Jagiellonian Univ, Malopolska Ctr Biotechnol, PL-30387 Krakow, Poland. | - |
local.uhasselt.international | yes | - |
item.contributor | Boon, Maarten | - |
item.contributor | Ceyssens, Pieter-Jan | - |
item.contributor | Voet, Marleen | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Andreeva, Julia | - |
item.contributor | Ghilarov, Dmitry | - |
item.contributor | Severinov, Konstantin | - |
item.contributor | Lavigne, Rob | - |
item.contributor | Wagemans, Jeroen | - |
item.contributor | De Smet, Jeroen | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2022 | - |
item.fullcitation | Boon, Maarten; Ceyssens, Pieter-Jan; Voet, Marleen; NOBEN, Jean-Paul; Andreeva, Julia; Ghilarov, Dmitry; Severinov, Konstantin; Lavigne, Rob; Wagemans, Jeroen & De Smet, Jeroen (2021) The bacteriophage LUZ24 “Igy” peptide inhibits the Pseudomonas DNA gyrase. In: CELL REPORTS, 36 (8) (Art N° 109567). | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 2211-1247 | - |
crisitem.journal.eissn | 2211-1247 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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1-s20-S2211124721010019-main.pdf | Published version | 1.92 MB | Adobe PDF | View/Open |
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