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http://hdl.handle.net/1942/34841
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DC Field | Value | Language |
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dc.contributor.author | VAN ZEEBROECK, Lauren | - |
dc.contributor.author | ARROYO HORNERO, Rebeca | - |
dc.contributor.author | FERNANDES CORTE-REAL, Beatriz | - |
dc.contributor.author | HAMAD, Ibrahim | - |
dc.contributor.author | Meissner, Torsten B. | - |
dc.contributor.author | KLEINEWIETFELD, Markus | - |
dc.date.accessioned | 2021-09-10T08:01:38Z | - |
dc.date.available | 2021-09-10T08:01:38Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-09-09T08:39:45Z | - |
dc.identifier.citation | FRONTIERS IN IMMUNOLOGY, 12 (Art N° 655122) | - |
dc.identifier.uri | http://hdl.handle.net/1942/34841 | - |
dc.description.abstract | FOXP3(+) regulatory T cells (Tregs) are central for maintaining peripheral tolerance and immune homeostasis. Because of their immunosuppressive characteristics, Tregs are a potential therapeutic target in various diseases such as autoimmunity, transplantation and infectious diseases like COVID-19. Numerous studies are currently exploring the potential of adoptive Treg therapy in different disease settings and novel genome editing techniques like CRISPR/Cas will likely widen possibilities to strengthen its efficacy. However, robust and expeditious protocols for genome editing of human Tregs are limited. Here, we describe a rapid and effective protocol for reaching high genome editing efficiencies in human Tregs without compromising cell integrity, suitable for potential therapeutic applications. By deletion of IL2RA encoding for IL-2 receptor alpha-chain (CD25) in Tregs, we demonstrated the applicability of the method for downstream functional assays and highlighted the importance for CD25 for in vitro suppressive function of human Tregs. Moreover, deletion of IL6RA (CD126) in human Tregs elicits cytokine unresponsiveness and thus may prevent IL-6-mediated instability of Tregs, making it an attractive target to potentially boost functionality in settings of adoptive Treg therapies to contain overreaching inflammation or autoimmunity. Thus, our rapid and efficient protocol for genome editing in human Tregs may advance possibilities for Treg-based cellular therapies. | - |
dc.description.sponsorship | European Research Council (ERC) under the European Union [640116]; SALK-grant from the government of Flanders; Research Foundation Flanders, Belgium (FWO) | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject.other | regulatory T cell; CD4; IL6R; CRISPR; human; genome editing; COVID-19; | - |
dc.subject.other | autoimmunity | - |
dc.title | Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 12 | - |
local.format.pages | 14 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Kleinewietfeld, M (corresponding author), Hasselt Univ, Vlaams Inst Biotechnol VIB, Lab Translat Immunomod, Ctr Inflammat Res IRC, Diepenbeek, Belgium.; Kleinewietfeld, M (corresponding author), Hasselt Univ, Biomed Res Inst, Dept Immunol, Diepenbeek, Belgium. | - |
dc.description.notes | markus.kleinewielfeld@uhassell.vib.be | - |
local.publisher.place | AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 655122 | - |
dc.identifier.doi | 10.3389/fimmu.2021.655122 | - |
dc.identifier.isi | WOS:000691310000001 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Van Zeebroeck, Lauren; Hornero, Rebeca Arroyo; Corte-Real, Beatriz F.; Hamad, Ibrahim; Kleinewietfeld, Markus] Hasselt Univ, Vlaams Inst Biotechnol VIB, Lab Translat Immunomod, Ctr Inflammat Res IRC, Diepenbeek, Belgium. | - |
local.description.affiliation | [Van Zeebroeck, Lauren; Hornero, Rebeca Arroyo; Corte-Real, Beatriz F.; Hamad, Ibrahim; Kleinewietfeld, Markus] Hasselt Univ, Biomed Res Inst, Dept Immunol, Diepenbeek, Belgium. | - |
local.description.affiliation | [Meissner, Torsten B.] Harvard Med Sch, Dept Surg, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA. | - |
local.uhasselt.international | yes | - |
item.accessRights | Open Access | - |
item.contributor | VAN ZEEBROECK, Lauren | - |
item.contributor | ARROYO HORNERO, Rebeca | - |
item.contributor | FERNANDES CORTE-REAL, Beatriz | - |
item.contributor | HAMAD, Ibrahim | - |
item.contributor | Meissner, Torsten B. | - |
item.contributor | KLEINEWIETFELD, Markus | - |
item.fulltext | With Fulltext | - |
item.fullcitation | VAN ZEEBROECK, Lauren; ARROYO HORNERO, Rebeca; FERNANDES CORTE-REAL, Beatriz; HAMAD, Ibrahim; Meissner, Torsten B. & KLEINEWIETFELD, Markus (2021) Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells. In: FRONTIERS IN IMMUNOLOGY, 12 (Art N° 655122). | - |
item.validation | ecoom 2022 | - |
crisitem.journal.issn | 1664-3224 | - |
crisitem.journal.eissn | 1664-3224 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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fimmu-12-655122.pdf | Published version | 6.86 MB | Adobe PDF | View/Open |
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