Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/3502
Title: | Oxidative damage and defense mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper | Authors: | Weckx, Jolanda CLIJSTERS, Herman |
Issue Date: | 1996 | Publisher: | MUNKSGAARD INT PUBL LTD | Source: | PHYSIOLOGIA PLANTARUM, 96(3). p. 506-512 | Abstract: | We studied the sequence of several metabolic reactions, representative for oxidative damage and protection, in primary leaves of Phaseolus vulgaris (cv. Limburgse vroege) as a function of root assimilation of a toxic sublethal Cu concentration (630 mu M). A transient increase of products of membrane peroxidation was observed in the primary leaves during the period of Cu uptake. This rise was mainly due to the oxidizing properties of copper itself and not to a stimulation of the lipoxygenase (EC 1.13.11.12) activity. In our experimental conditions, membrane lipid peroxidation and K+-leakage were not directly related; during at least three days after Cu application to the roots, when products of lipid peroxidation were already detected in the leaf, permeability of the cytoplasmic membrane for K+ was improved. However, Cu stimulated the capacity of catalase (EC 1.11.1.6) and ascorbate peroxidase (EC 1.11.1.11). These enzymes protect the tissue against oxidative stress since at least the hydrogen peroxide content was significantly reduced. Superoxide dismutase (EC 1.15.1.1) was not involved in this defense mechanism. | Notes: | LIMBURGS UNIV CENTRUM,DEPT SBG,B-3590 DIEPENBEEK,BELGIUM. | Keywords: | ascorbate peroxidase; bean; catalase; copper toxicity; defense mechanisms; hydrogen peroxide; lipid peroxidation; membrane permeability; oxidative damage; Phaseolus vulgaris; primary leaves; superoxide dismutase | Document URI: | http://hdl.handle.net/1942/3502 | DOI: | 10.1034/j.1399-3054.1996.960322.x | ISI #: | A1996UG64300022 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Show full item record
WEB OF SCIENCETM
Citations
334
checked on Oct 4, 2024
Page view(s)
104
checked on Nov 7, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.