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http://hdl.handle.net/1942/35091
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DC Field | Value | Language |
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dc.contributor.advisor | FRAUSSEN, Judith | |
dc.contributor.author | Bovens, Becky | |
dc.date.accessioned | 2021-09-13T13:03:13Z | - |
dc.date.available | 2021-09-13T13:03:13Z | - |
dc.date.issued | 2021 | |
dc.identifier.uri | http://hdl.handle.net/1942/35091 | - |
dc.description.abstract | Spinal cord injury (SCI) is a devastating condition, leading to sensory and motor function loss. SCI disrupts the blood-spinal cord barrier, releasing SCI-related proteins in the blood stream, triggering autoimmune responses and the production of autoantibodies. Since there are no predictive strategies for the SCI-outcome, these SCI-induced autoantibodies can be valuable as prognostic disease markers, especially in patients with worsening of the SCI. Our aim was to identify a full spectrum of SCI-induced autoantibodies from SCI patients’ plasma using serological antigen selection (SAS). Immunoglobulin (Ig)M and IgG levels in SCI patients’ plasma were determined 0-4 days post-injury (DPI) and 20-33 DPI using ELISA. Next, IgM ELISA and SAS procedures were optimised. SAS used a cDNA phage display library expressing spinal cord proteins, which was screened for SCI-induced autoantibody reactivity using pooled plasma of 12 SCI patients with known worsening of the SCI. Phage expressing antigens to which SCI-induced autoantibodies bound were selected. Selected antigenic targets were characterised using colony PCR and DNA fingerprinting. SCI patients’ total plasma IgM levels were significantly increased (p=0.0491) 20-33 DPI compared to 0-4 DPI, in contrast, IgG levels remained stable (p=0.3474). SAS and phage ELISA data suggested an increased selection of phage expressing antigens with SCI-induced autoantibody reactivity. DNA fingerprinting of the selected clones identified five digestion patterns, confirming enrichment of phage. | |
dc.format.mimetype | Application/pdf | |
dc.language | en | |
dc.publisher | tUL | |
dc.title | Identification of spinal cord injury-induced autoantibodies as prognostic biomarkers using serological antigen selection | |
dc.type | Theses and Dissertations | |
local.bibliographicCitation.jcat | T2 | |
dc.description.notes | Master of Biomedical Sciences-Molecular Mechanisms in Health and Disease | |
local.type.specified | Master thesis | |
item.fullcitation | Bovens, Becky (2021) Identification of spinal cord injury-induced autoantibodies as prognostic biomarkers using serological antigen selection. | - |
item.fulltext | With Fulltext | - |
item.contributor | Bovens, Becky | - |
item.accessRights | Open Access | - |
Appears in Collections: | Master theses |
Files in This Item:
File | Description | Size | Format | |
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c1230f7b-bf54-4aa3-932e-0928572e4b02.pdf | 1.12 MB | Adobe PDF | View/Open |
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