Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3516
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dc.contributor.authorAndersen, H.K.-
dc.contributor.authorURBACH, V-
dc.contributor.authorVAN KERKHOVE, Emmy-
dc.contributor.authorPROSSER, E-
dc.contributor.authorHARVEY, BJ-
dc.date.accessioned2007-11-28T16:26:23Z-
dc.date.available2007-11-28T16:26:23Z-
dc.date.issued1995-
dc.identifier.citationPFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 431(1). p. 52-65-
dc.identifier.issn0031-6768-
dc.identifier.urihttp://hdl.handle.net/1942/3516-
dc.description.abstractWith the single-channel patch-clamp technique we have identified Ca2+-sensitive, high-conductance (maxi) K+ channels in the basolateral membrane (BLM) of exocrine gland cells in frog skin, Under resting conditions, maxi K+ channels were normally quiescent, but they were activated by muscarinic agonists or by high serosal K+. In excised inside-out patches and with symmetrical 140 mmol/l K+, single-channel conductance was 200 pS and the channel exhibited a high selectivity for K+ over Na+. Depolarization of the BLM increased maxi K+ channel activity. Increasing cytosolic free Ca2+ (by addition of 100 nmol/l thapsigargin to the bathing solution of cell-attached patches also increased channel activity, whereas thapsigargin had no effect when added to excised inside-out patches, An increase in cytosolic free Ca2+ directly activated channel activity in a voltage-dependent manner, Maxi K+ channel activity was sensitive to changes in intracellular pH, with maximal activity at pH 7.4 and decreasing activities following acidification and alkalinization. Maxi K+ channel outward current was reversibly blocked by micromolar concentrations of Ba2+ from the cytosolic and extracellular site, and was irreversibly blocked by micromolar concentrations of charybdotoxin and kaliotoxin from the extracellular site in outside-out patches.-
dc.language.isoen-
dc.publisherSPRINGER VERLAG-
dc.subject.otherCALCIUM; FROG SKIN GLANDS; MAXI K+ CHANNEL; PATCH CLAMP; PH; THAPSIGARGIN-
dc.titleMAXI K+ CHANNELS IN THE BASOLATERAL MEMBRANE OF THE EXOCRINE FROG-SKIN GLAND REGULATED BY INTRACELLULAR CALCIUM AND PH-
dc.typeJournal Contribution-
dc.identifier.epage65-
dc.identifier.issue1-
dc.identifier.spage52-
dc.identifier.volume431-
local.format.pages14-
dc.description.notesNATL UNIV IRELAND UNIV COLL CORK,DEPT PHYSIOL,CELLULAR PHYSIOL RES UNIT,CORK,IRELAND. LIMBURGS UNIV CENTRUM,DEPT MBW FYSIOL,B-3590 DIEPENBEEK,BELGIUM.ANDERSEN, HK, AUGUST KROGH INST,ZOOPHYSIOL LAB,UNIV PK 13,DK-2100 COPENHAGEN O,DENMARK.-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.bibliographicCitation.oldjcatA1-
dc.identifier.isiA1995TG33200007-
item.fulltextNo Fulltext-
item.accessRightsClosed Access-
item.fullcitationAndersen, H.K.; URBACH, V; VAN KERKHOVE, Emmy; PROSSER, E & HARVEY, BJ (1995) MAXI K+ CHANNELS IN THE BASOLATERAL MEMBRANE OF THE EXOCRINE FROG-SKIN GLAND REGULATED BY INTRACELLULAR CALCIUM AND PH. In: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 431(1). p. 52-65.-
item.contributorVAN KERKHOVE, Emmy-
item.contributorHARVEY, BJ-
item.contributorPROSSER, E-
item.contributorAndersen, H.K.-
item.contributorURBACH, V-
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