Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/35380
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | YADAV, Anu | - |
dc.contributor.author | Maertens, Laurens | - |
dc.contributor.author | Meese, Tim | - |
dc.contributor.author | Van Nieuwerburgh, Filip | - |
dc.contributor.author | Mysara, Mohamed | - |
dc.contributor.author | Leys, Natalie | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.contributor.author | Janssen, Paul Jaak | - |
dc.date.accessioned | 2021-09-15T15:49:57Z | - |
dc.date.available | 2021-09-15T15:49:57Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-09-09T13:14:40Z | - |
dc.identifier.citation | MICROORGANISMS, 9 (8) (Art N° 1626) | - |
dc.identifier.uri | http://hdl.handle.net/1942/35380 | - |
dc.description.abstract | Two morphotypes of the cyanobacterial Limnospira indica (formerly Arthrospira sp.) strain PCC 8005, denoted as P2 (straight trichomes) and P6 (helical trichomes), were subjected to chronic gamma radiation from spent nuclear fuel (SNF) rods at a dose rate of ca. 80 Gy center dot h(-1) for one mass doubling period (approximately 3 days) under continuous light with photoautotrophic metabolism fully active. Samples were taken for post-irradiation growth recovery and RNA-Seq transcriptional analysis at time intervals of 15, 40, and 71.5 h corresponding to cumulative doses of ca. 1450, 3200, and 5700 Gy, respectively. Both morphotypes, which were previously reported by us to display different antioxidant capacities and differ at the genomic level in 168 SNPs, 48 indels and 4 large insertions, recovered equally well from 1450 and 3200 Gy. However, while the P2 straight type recovered from 5700 Gy by regaining normal growth within 6 days, the P6 helical type took about 13 days to recover from this dose, indicating differences in their radiation tolerance and response. To investigate these differences, P2 and P6 cells exposed to the intermediate dose of gamma radiation (3200 Gy) were analyzed for differential gene expression by RNA-Seq analysis. Prior to batch normalization, a total of 1553 genes (887 and 666 of P2 and P6, respectively, with 352 genes in common) were selected based on a two-fold change in expression and a false discovery rate FDR smaller or equal to 0.05. About 85% of these 1553 genes encoded products of yet unknown function. Of the 229 remaining genes, 171 had a defined function while 58 genes were transcribed into non-coding RNA including 21 tRNAs (all downregulated). Batch normalization resulted in 660 differentially expressed genes with 98 having a function and 32 encoding RNA. From PCC 8005-P2 and PCC 8005-P6 expression patterns, it emerges that although the cellular routes used by the two substrains to cope with ionizing radiation do overlap to a large extent, both strains displayed a distinct preference of priorities. | - |
dc.description.sponsorship | Funding: This work was in part financed through a PhD grant for A. Yadav provided by SCKCEN. Acknowledgments: We wish to thank Andrei Goussarov (SCKCEN—Nuclear Engineering Office) for providing access to nuclear reactor BR2 and its irradiation facilities, Steven Van Loven and Kris Thijs (SCKCEN—NEO) for loading and unloading biological samples in the GEUSE II vessel, Ilse Coninx (SCKCEN—Microbiology Research Unit) for maintenance of bacterial cultures, RNA extractions, and general labwork, Eric Boogers (SCKCEN—Dosimetric and Callibration Services) for design and build of the LED lighting towers, and Jürgen Verlinden (SCKCEN—Mechanical Workshop) and Çelik Yurdunaz (SCKCEN—Nuclear Systems Physics) for gamma ray shielding calculations and design. | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.rights | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
dc.subject.other | Limnospira | - |
dc.subject.other | Arthrospira | - |
dc.subject.other | gamma radiation | - |
dc.subject.other | expression analysis | - |
dc.subject.other | RNA-Seq | - |
dc.subject.other | radiation resistance | - |
dc.subject.other | morphology | - |
dc.subject.other | genomics | - |
dc.subject.other | genetic response | - |
dc.title | Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 8 | - |
dc.identifier.volume | 9 | - |
local.format.pages | 32 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Janssen, PJ (corresponding author), Belgian Nucl Res Ctr SCKCEN, Interdisciplinary Biosci, Microbiol Unit, B-2400 Mol, Belgium. | - |
dc.description.notes | anuiisermohali@gmail.com; laurens.maertens@sckcen.be; | - |
dc.description.notes | tim.meese@ugent.be; filip.vannieuwerburgh@ugent.be; | - |
dc.description.notes | mohamed.mysara.ahmed@sckcen.be; natalie.leys@sckcen.be; | - |
dc.description.notes | ann.cuypers@uhasselt.be; pjanssen@sckcen.be | - |
local.publisher.place | ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 1626 | - |
dc.identifier.doi | 10.3390/microorganisms9081626 | - |
dc.identifier.isi | WOS:000689442200001 | - |
dc.contributor.orcid | Cuypers, Ann/0000-0002-0171-0245 | - |
dc.identifier.eissn | 2076-2607 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Yadav, Anu; Maertens, Laurens; Mysara, Mohamed; Leys, Natalie; Janssen, Paul Jaak] Belgian Nucl Res Ctr SCKCEN, Interdisciplinary Biosci, Microbiol Unit, B-2400 Mol, Belgium. | - |
local.description.affiliation | [Yadav, Anu; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Environm Biol, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Maertens, Laurens] Univ Namur, Narilis Inst, Res Unit Biol Microorganisms URBM, B-5000 Namur, Belgium. | - |
local.description.affiliation | [Meese, Tim; Van Nieuwerburgh, Filip] Univ Ghent, Lab Pharmaceut Biotechnol, B-9000 Ghent, Belgium. | - |
local.uhasselt.international | no | - |
item.fulltext | With Fulltext | - |
item.contributor | YADAV, Anu | - |
item.contributor | Maertens, Laurens | - |
item.contributor | Meese, Tim | - |
item.contributor | Van Nieuwerburgh, Filip | - |
item.contributor | Mysara, Mohamed | - |
item.contributor | Leys, Natalie | - |
item.contributor | CUYPERS, Ann | - |
item.contributor | Janssen, Paul Jaak | - |
item.accessRights | Open Access | - |
item.fullcitation | YADAV, Anu; Maertens, Laurens; Meese, Tim; Van Nieuwerburgh, Filip; Mysara, Mohamed; Leys, Natalie; CUYPERS, Ann & Janssen, Paul Jaak (2021) Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle. In: MICROORGANISMS, 9 (8) (Art N° 1626). | - |
item.validation | ecoom 2022 | - |
crisitem.journal.eissn | 2076-2607 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
microorganisms-09-01626-v2.pdf | Published version | 1.41 MB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
2
checked on Oct 6, 2024
Page view(s)
30
checked on Sep 7, 2022
Download(s)
12
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.