Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/35392
Title: | Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium | Authors: | BORGO, Lucelia SILVEIRA RABELO, Flavio Frasson Budzinski, Ilara Gabriela Cataldi, Thais Regiani Ramires, Thiago Gentil Carvalho Schaker, Patricia Dayane Ribas, Alessandra Ferreira Labate, Carlos Alberto Lavres, Jose CUYPERS, Ann Azevedo, Ricardo Antunes |
Issue Date: | 2022 | Publisher: | SPRINGER | Source: | JOURNAL OF PLANT GROWTH REGULATION, 41(7), p. 2846-2868 | Abstract: | Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 mu mol L-1 CdCl2 center dot H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L-1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure. | Notes: | Borgo, L (corresponding author), Univ Sao Paulo, Coll Agr Luiz Queiroz, BR-13418900 Piracicaba, Brazil.; Borgo, L (corresponding author), Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13416000 Piracicaba, Brazil.; Borgo, L (corresponding author), Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium.; Borgo, L (corresponding author), Escola Super Agr Luis Dequeiroz, Dept Genet, Lab Genet Bioquim Plantas, Ave Padua Dias 11,Caixa Postal 9, BR-13418900 Piracicaba, SP, Brazil. borglucelia@gmail.com |
Keywords: | Glutathione; Metabolite profiling; Nicotiana tabacum L; Nutrient;homeostasis; Oxidative stress; P5CSF129A | Document URI: | http://hdl.handle.net/1942/35392 | ISSN: | 0721-7595 | e-ISSN: | 1435-8107 | DOI: | 10.1007/s00344-021-10480-6 | ISI #: | WOS:000692616600002 | Rights: | © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
s00344-021-10480-6.pdf Restricted Access | Published version | 1.44 MB | Adobe PDF | View/Open Request a copy |
WEB OF SCIENCETM
Citations
8
checked on Jun 25, 2024
Page view(s)
32
checked on Sep 7, 2022
Download(s)
6
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.