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http://hdl.handle.net/1942/35888
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DC Field | Value | Language |
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dc.contributor.author | Ceusters, N | - |
dc.contributor.author | CEUSTERS, Johan | - |
dc.contributor.author | Hurtado-Castano, N | - |
dc.contributor.author | Dever, LV | - |
dc.contributor.author | Boxall, SF | - |
dc.contributor.author | Knerova, J | - |
dc.contributor.author | Waller, JL | - |
dc.contributor.author | Rodick, R | - |
dc.contributor.author | Van den Ende, W | - |
dc.contributor.author | Hartwell, J | - |
dc.contributor.author | Borland, AM | - |
dc.contributor.editor | Beckles, Diane | - |
dc.date.accessioned | 2021-11-25T13:10:58Z | - |
dc.date.available | 2021-11-25T13:10:58Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-09-13T15:09:37Z | - |
dc.identifier.citation | Journal of experimental botany, 72 (12) , p. 4419 -4434 | - |
dc.identifier.uri | http://hdl.handle.net/1942/35888 | - |
dc.description.abstract | In plants with Crassulacean acid metabolism (CAM), it has been proposed that the requirement for nocturnal provision of phosphoenolpyruvate as a substrate for CO2 uptake has resulted in a re-routing of chloroplastic starch degradation from the amylolytic route to the phosphorolytic route. To test this hypothesis, we generated and characterized four independent RNAi lines of the obligate CAM species Kalanchoe fedtschenkoi with a >10-fold reduction in transcript abundance of plastidic alpha-glucan phosphorylase (PHS1). The rPHS1 lines showed diminished nocturnal starch degradation, reduced dark CO2 uptake, a reduction in diel water use efficiency (WUE), and an overall reduction in growth. A re-routing of starch degradation via the hydrolytic/amylolytic pathway was indicated by hyperaccumulation of maltose in all rPHS1 lines. Further examination indicated that whilst operation of the core circadian clock was not compromised, plasticity in modulating net dark CO2 uptake in response to changing photoperiods was curtailed. The data show that phosphorolytic starch degradation is critical for efficient operation of the CAM cycle and for optimizing WUE. This finding has clear relevance for ongoing efforts to engineer CAM into non-CAM species as a means of boosting crop WUE for a warmer, drier future. | - |
dc.description.sponsorship | This research was supported by a Marie Curie Fellowship granted to JC with additional support provided by Research Fund KU Leuven. NHC was funded by a grant from Colciencias. This work was supported in part by the Biotechnology and Biological Sciences Research Council, UK (BBSRC grant no. BB/F009313/1 awarded to JH) and in part by the U.S. Department of Energy (DOE) Office of Science, Genomic Science Program under Award Number DE-SC0008834. The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of the DOE. | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.rights | The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | - |
dc.subject.other | CAM | - |
dc.subject.other | gas exchange | - |
dc.subject.other | hydrolytic pathway | - |
dc.subject.other | phosphorolytic pathway | - |
dc.subject.other | starch | - |
dc.title | Phosphorolytic degradation of leaf starch via plastidic alpha-glucan phosphorylase leads to optimized plant growth and water use efficiency over the diel phases of Crassulacean acid metabolism | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 4434 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 4419 | - |
dc.identifier.volume | 72 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1093/jxb/erab132 | - |
dc.identifier.pmid | 33754643 | - |
dc.identifier.isi | 000661482800017 | - |
local.provider.type | Web of Science | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2022 | - |
item.accessRights | Open Access | - |
item.fullcitation | Ceusters, N; CEUSTERS, Johan; Hurtado-Castano, N; Dever, LV; Boxall, SF; Knerova, J; Waller, JL; Rodick, R; Van den Ende, W; Hartwell, J & Borland, AM (2021) Phosphorolytic degradation of leaf starch via plastidic alpha-glucan phosphorylase leads to optimized plant growth and water use efficiency over the diel phases of Crassulacean acid metabolism. In: Journal of experimental botany, 72 (12) , p. 4419 -4434. | - |
item.contributor | Ceusters, N | - |
item.contributor | CEUSTERS, Johan | - |
item.contributor | Hurtado-Castano, N | - |
item.contributor | Dever, LV | - |
item.contributor | Boxall, SF | - |
item.contributor | Knerova, J | - |
item.contributor | Waller, JL | - |
item.contributor | Rodick, R | - |
item.contributor | Van den Ende, W | - |
item.contributor | Hartwell, J | - |
item.contributor | Borland, AM | - |
item.contributor | Beckles, Diane | - |
crisitem.journal.issn | 0022-0957 | - |
crisitem.journal.eissn | 1460-2431 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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erab132.pdf | Published version | 3.29 MB | Adobe PDF | View/Open |
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