Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/35981
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | OLUSOJI, Oluwafemi | - |
dc.contributor.author | Spaak, Jurg W. | - |
dc.contributor.author | Holmes, Mark | - |
dc.contributor.author | NEYENS, Thomas | - |
dc.contributor.author | AERTS, Marc | - |
dc.contributor.author | De Laender, Frederik | - |
dc.date.accessioned | 2021-11-30T16:00:39Z | - |
dc.date.available | 2021-11-30T16:00:39Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-10-28T11:31:57Z | - |
dc.identifier.citation | Ecological modelling, 460 (Art N° 109743) | - |
dc.identifier.uri | http://hdl.handle.net/1942/35981 | - |
dc.description.abstract | Flow cytometry is often employed in ecology to measure traits and population size of bacteria and phytoplankton. This technique allows measuring millions of particles in a relatively small amount of time. However, distinguishing between different populations is not a straightforward task. Gating is a process in the identification of particles measured in flow cytometry. Gates can either be created manually using known characteristics of these particles, or by using automated clustering techniques. Available automated techniques implemented in statistical packages for flow cytometry are primarily developed for medicinal applications, while only two exist for phytoplankton. cyanoFilter is an R package built to identify phytoplankton populations from flow cytometry data. The package also integrates gating functions from two other automated algorithms. It also provides a gating accuracy test function that can be used to determine the accuracy of a desired gating function if monoculture flowcytometry data is available. The central algorithm in the package exploits observed pigmentation and granularity of phytoplankton cells. We demonstrate how its performance depends on strain similarity, using a model system of six cyanobacteria strains. Using the same system, we compare the performance of the central gating function in the package to similar functions in other packages. | - |
dc.description.sponsorship | bilateral co-operation (BOF); Universite de Namur; ARC, Belgium grant DIVERCE [18/23-095]; Fund for Scientific Research, FNRS, Belgium Fonds de la Recherche Scientifique - FNRSFWO [PDR T.0048.16] | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.rights | © 2021 Elsevier B.V. All rights reserved | - |
dc.subject.other | Flow cytometry; Phytoplankton; Gating; Software; Ecology | - |
dc.title | cyanoFilter: An R package to identify phytoplankton populations from flow cytometry data using cell pigmentation and granularity | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 460 | - |
local.format.pages | 7 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Olusoji, OD (corresponding author), Hasselt Univ, Data Sci Inst, Ctr Stat, B-3500 Hasselt, Belgium. | - |
dc.description.notes | oluwafemi.olusoji@uhasselt.be | - |
local.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 109743 | - |
dc.identifier.doi | 10.1016/j.ecolmodel.2021.109743 | - |
dc.identifier.isi | WOS:000703867600002 | - |
dc.contributor.orcid | Holmes, Mark/0000-0002-0273-0483 | - |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Olusoji, Oluwafemi D.; Neyens, Thomas; Aerts, Marc] Hasselt Univ, Data Sci Inst, Ctr Stat, B-3500 Hasselt, Belgium. | - |
local.description.affiliation | [Olusoji, Oluwafemi D.; Spaak, Jurg W.; Holmes, Mark; De Laender, Frederik] Univ Namur, Res Unit Environm & Evolutionary Biol URBE, Inst Life Earth Environm ILEE, Namur Inst Complex Syst NAXYS, B-5020 Namur, Belgium. | - |
local.uhasselt.international | no | - |
item.contributor | OLUSOJI, Oluwafemi | - |
item.contributor | Spaak, Jurg W. | - |
item.contributor | Holmes, Mark | - |
item.contributor | NEYENS, Thomas | - |
item.contributor | AERTS, Marc | - |
item.contributor | De Laender, Frederik | - |
item.fullcitation | OLUSOJI, Oluwafemi; Spaak, Jurg W.; Holmes, Mark; NEYENS, Thomas; AERTS, Marc & De Laender, Frederik (2021) cyanoFilter: An R package to identify phytoplankton populations from flow cytometry data using cell pigmentation and granularity. In: Ecological modelling, 460 (Art N° 109743). | - |
item.accessRights | Closed Access | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2022 | - |
crisitem.journal.issn | 0304-3800 | - |
crisitem.journal.eissn | 1872-7026 | - |
Appears in Collections: | Research publications |
WEB OF SCIENCETM
Citations
1
checked on Apr 30, 2024
Page view(s)
36
checked on Sep 7, 2022
Download(s)
4
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.