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http://hdl.handle.net/1942/36099
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DC Field | Value | Language |
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dc.contributor.author | Van Cleemput, Jolien | - |
dc.contributor.author | van Snippenberg, Willem | - |
dc.contributor.author | Lambrechts, Laurens | - |
dc.contributor.author | Dendooven, Amelie | - |
dc.contributor.author | D'ONOFRIO, Valentino | - |
dc.contributor.author | Couck, Liesbeth | - |
dc.contributor.author | Trypsteen, Wim | - |
dc.contributor.author | Vanrusselt, Jan | - |
dc.contributor.author | Theuns, Sebastiaan | - |
dc.contributor.author | Vereecke, Nick | - |
dc.contributor.author | van den Bosch, Thierry P. P. | - |
dc.contributor.author | Lammens, Martin | - |
dc.contributor.author | DRIESSEN, Ann | - |
dc.contributor.author | ACHTEN, Ruth | - |
dc.contributor.author | Bracke, Ken R. | - |
dc.contributor.author | VAN DEN BROECK, Wim | - |
dc.contributor.author | Von der Thusen, Jan | - |
dc.contributor.author | Nauwynck, Hans | - |
dc.contributor.author | Van Dorpe, Jo | - |
dc.contributor.author | Gerlo, Sarah | - |
dc.contributor.author | Maes, Piet | - |
dc.contributor.author | COX, Janneke | - |
dc.contributor.author | Vandekerckhove, Linos | - |
dc.date.accessioned | 2021-12-08T16:07:40Z | - |
dc.date.available | 2021-12-08T16:07:40Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2021-12-08T12:38:53Z | - |
dc.identifier.citation | NATURE COMMUNICATIONS, 12 (1) (Art N° 6612) | - |
dc.identifier.uri | http://hdl.handle.net/1942/36099 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is not always confined to the respiratory system, as it impacts people on a broad clinical spectrum from asymptomatic to severe systemic manifestations resulting in death. Further, accumulation of intra-host single nucleotide variants during prolonged SARS-CoV-2 infection may lead to emergence of variants of concern (VOCs). Still, information on virus infectivity and intra-host evolution across organs is sparse. We report a detailed virological analysis of thirteen postmortem coronavirus disease 2019 (COVID-19) cases that provides proof of viremia and presence of replication-competent SARS-CoV-2 in extrapulmonary organs of immunocompromised patients, including heart, kidney, liver, and spleen (NCT04366882). In parallel, we identify organ-specific SARS-CoV-2 genome diversity and mutations of concern N501Y, T1027I, and Y453F, while the patient had died long before reported emergence of VOCs. These mutations appear in multiple organs and replicate in Vero E6 cells, highlighting their infectivity. Finally, we show two stages of fatal disease evolution based on disease duration and viral loads in lungs and plasma. Our results provide insights about the pathogenesis and intra-host evolution of SARS-CoV-2 and show that COVID-19 treatment and hygiene measures need to be tailored to specific needs of immunocompromised patients, even when respiratory symptoms cease. Here the authors provide a detailed virological analysis of thirteen postmortem COVID-19 cases, including presence of replication-competent SARS-CoV-2 in extrapulmonary organs and tissue-specific patterns of SARS-CoV-2 genome diversity of an immunocompromised patient. | - |
dc.description.sponsorship | FWO COVID-19 grantsFWO [G0G2620N, G0G4520N]; Research Foundation Flanders (FWO)FWO [12ZB921N, 1S29220N]; ZonMwNetherlands Organization for Health Research and Development [10430012010016] | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License | - |
dc.title | Organ-specific genome diversity of replication-competent SARS-CoV-2 | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 1 | - |
dc.identifier.volume | 12 | - |
local.format.pages | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Van Cleemput, J; Vandekerckhove, L (corresponding author), Univ Ghent, Ghent Univ Hosp, HIV Cure Res Ctr, Dept Internal Med & Pediat, Ghent, Belgium. | - |
dc.description.notes | jolien.vancleemput@ugent.be; linos.vandekerckhove@ugent.be | - |
local.publisher.place | HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 6612 | - |
dc.identifier.doi | 10.1038/s41467-021-26884-7 | - |
dc.identifier.isi | WOS:000719546000026 | - |
dc.contributor.orcid | Vereecke, Nick/0000-0001-5367-9756 | - |
dc.identifier.eissn | - | |
local.provider.type | wosris | - |
local.uhasselt.uhpub | yes | - |
local.description.affiliation | [Van Cleemput, Jolien; van Snippenberg, Willem; Lambrechts, Laurens; Trypsteen, Wim; Gerlo, Sarah; Vandekerckhove, Linos] Univ Ghent, Ghent Univ Hosp, HIV Cure Res Ctr, Dept Internal Med & Pediat, Ghent, Belgium. | - |
local.description.affiliation | [Lambrechts, Laurens] Univ Ghent, Fac Biosci Engn, Dept Data Anal & Math Modelling, BioBix, Ghent, Belgium. | - |
local.description.affiliation | [Dendooven, Amelie; Van Dorpe, Jo] Univ Ghent, Ghent Univ Hosp, Dept Pathol, Ghent, Belgium. | - |
local.description.affiliation | [Dendooven, Amelie; Lammens, Martin; Driessen, Ann; Achten, Ruth] Antwerp Univ Hosp, Dept Pathol, Edegem, Belgium. | - |
local.description.affiliation | [Dendooven, Amelie; Lammens, Martin; Driessen, Ann; Achten, Ruth] Univ Antwerp, Fac Med & Hlth Sci, Antwerp, Belgium. | - |
local.description.affiliation | [D'Onofrio, Valentino; Cox, Janneke] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. | - |
local.description.affiliation | [D'Onofrio, Valentino; Cox, Janneke] Jessa Hosp, Dept Infect Dis & Immun, Hasselt, Belgium. | - |
local.description.affiliation | [Couck, Liesbeth; Van den Broeck, Wim] Univ Ghent, Fac Vet Med, Dept Morphol, Merelbeke, Belgium. | - |
local.description.affiliation | [Vanrusselt, Jan] Jessa Hosp, Dept Radiol, Hasselt, Belgium. | - |
local.description.affiliation | [Theuns, Sebastiaan; Vereecke, Nick] PathoSense BV, Lier, Belgium. | - |
local.description.affiliation | [Theuns, Sebastiaan; Vereecke, Nick; Nauwynck, Hans] Univ Ghent, Fac Vet Med, Dept Virol Parasitol & Immunol, Merelbeke, Belgium. | - |
local.description.affiliation | [van den Bosch, Thierry P. P.; Von der Thusen, Jan] Erasmus MC, Dept Pathol, Rotterdam, Netherlands. | - |
local.description.affiliation | [Achten, Ruth] Jessa Hosp, Dept Pathol, Hasselt, Belgium. | - |
local.description.affiliation | [Bracke, Ken R.] Univ Ghent, Ghent Univ Hosp, Dept Resp Med, Lab Translat Res Obstruct Pulm Dis, Ghent, Belgium. | - |
local.description.affiliation | [Gerlo, Sarah] Univ Ghent, Dept Biomol Med, Ghent, Belgium. | - |
local.description.affiliation | [Maes, Piet] Katholieke Univ Leuven, Rega Inst, Dept Microbiol Immunol & Transplantat, Lab Clin & Epidemiol Virol, Leuven, Belgium. | - |
local.uhasselt.international | yes | - |
item.validation | ecoom 2022 | - |
item.contributor | Van Cleemput, Jolien | - |
item.contributor | van Snippenberg, Willem | - |
item.contributor | Lambrechts, Laurens | - |
item.contributor | Dendooven, Amelie | - |
item.contributor | D'ONOFRIO, Valentino | - |
item.contributor | Couck, Liesbeth | - |
item.contributor | Trypsteen, Wim | - |
item.contributor | Vanrusselt, Jan | - |
item.contributor | Theuns, Sebastiaan | - |
item.contributor | Vereecke, Nick | - |
item.contributor | van den Bosch, Thierry P. P. | - |
item.contributor | Lammens, Martin | - |
item.contributor | DRIESSEN, Ann | - |
item.contributor | ACHTEN, Ruth | - |
item.contributor | Bracke, Ken R. | - |
item.contributor | VAN DEN BROECK, Wim | - |
item.contributor | Von der Thusen, Jan | - |
item.contributor | Nauwynck, Hans | - |
item.contributor | Van Dorpe, Jo | - |
item.contributor | Gerlo, Sarah | - |
item.contributor | Maes, Piet | - |
item.contributor | COX, Janneke | - |
item.contributor | Vandekerckhove, Linos | - |
item.accessRights | Open Access | - |
item.fullcitation | Van Cleemput, Jolien; van Snippenberg, Willem; Lambrechts, Laurens; Dendooven, Amelie; D'ONOFRIO, Valentino; Couck, Liesbeth; Trypsteen, Wim; Vanrusselt, Jan; Theuns, Sebastiaan; Vereecke, Nick; van den Bosch, Thierry P. P.; Lammens, Martin; DRIESSEN, Ann; ACHTEN, Ruth; Bracke, Ken R.; VAN DEN BROECK, Wim; Von der Thusen, Jan; Nauwynck, Hans; Van Dorpe, Jo; Gerlo, Sarah; Maes, Piet; COX, Janneke & Vandekerckhove, Linos (2021) Organ-specific genome diversity of replication-competent SARS-CoV-2. In: NATURE COMMUNICATIONS, 12 (1) (Art N° 6612). | - |
item.fulltext | With Fulltext | - |
crisitem.journal.eissn | 2041-1723 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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s41467-021-26884-7.pdf | Published version | 7.98 MB | Adobe PDF | View/Open |
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