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http://hdl.handle.net/1942/3610
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DC Field | Value | Language |
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dc.contributor.author | ZHANG, SL | - |
dc.contributor.author | LEYSSENS, Anne | - |
dc.contributor.author | VAN KERKHOVE, Emmy | - |
dc.contributor.author | WELTENS, R | - |
dc.contributor.author | VAN DRIESSCHE, Willy | - |
dc.contributor.author | STEELS, Paul | - |
dc.date.accessioned | 2007-11-29T09:28:02Z | - |
dc.date.available | 2007-11-29T09:28:02Z | - |
dc.date.issued | 1994 | - |
dc.identifier.citation | PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 426(3-4). p. 288-295 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3610 | - |
dc.description.abstract | Cellular and luminal pH of isolated ant Malpighian tubules were measured in different bath K+ concentrations using double-barrelled pH microelectrodes. The electrochemical gradient for H+ across the basolateral and the apical cell membranes was estimated. In control Ringer (51 mmol/l K+) cell and luminal pH were alkaline with respect to the basolateral solution: 7.77 and 7.36, respectively, versus 7.25. On lowering basolateral K+ concentration to 5 mmol/l or increasing it to 113 mmol/l, luminal pH and to a lesser extent cell pH followed: luminal pH changed to 7.14 and 7.43 and cell pH to 7.69 and 7.82. respectively. In all conditions a cell inward electrochemical gradient for protons across both membranes was observed. Increasing basolateral K+ concentration, which was positively correlated with secretion rate, decreased the cell inwardly directed apical proton gradient; moreover, the apical membrane potential difference decreased as well, from -93 mV in 5 mmol/l K+ to -65 mV in 113 mmol/l K+. Therefore the turnover rate of the electrogenic active proton pump at the apical membrane is facilitated in a high basolateral K+ concentration. The calculated electromotive force of this pump is -159 mV. Comparing the proton with the K+ electrochemical gradient, taken from another study in the same experimental conditions, we find that the apical proton electrochemical gradient can drive K+ extrusion into the lumen for each value of secretion rate. | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER VERLAG | - |
dc.subject.other | ION-SENSITIVE MICROELECTRODES; BASOLATERAL POTASSIUM CONCENTRATION; SECRETION RATE | - |
dc.title | Electrophysiological evidence for the presence of an apical h+-atpase in malpighian tubules of formica-polyctena - intracellular and luminal ph measurements | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 295 | - |
dc.identifier.issue | 3-4 | - |
dc.identifier.spage | 288 | - |
dc.identifier.volume | 426 | - |
local.format.pages | 8 | - |
dc.description.notes | LIMBURGS UNIV CTR,DEPT MED BASISWETENSCHAPPEN,FYSIOL LAB,B-3590 DIEPENBEEK,BELGIUM. KATHOLIEKE UNIV LEUVEN,FYSIOL LAB,B-3000 LOUVAIN,BELGIUM. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1007/BF00374784 | - |
dc.identifier.isi | A1994MX01400016 | - |
item.fulltext | No Fulltext | - |
item.contributor | ZHANG, SL | - |
item.contributor | LEYSSENS, Anne | - |
item.contributor | VAN KERKHOVE, Emmy | - |
item.contributor | WELTENS, R | - |
item.contributor | VAN DRIESSCHE, Willy | - |
item.contributor | STEELS, Paul | - |
item.fullcitation | ZHANG, SL; LEYSSENS, Anne; VAN KERKHOVE, Emmy; WELTENS, R; VAN DRIESSCHE, Willy & STEELS, Paul (1994) Electrophysiological evidence for the presence of an apical h+-atpase in malpighian tubules of formica-polyctena - intracellular and luminal ph measurements. In: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 426(3-4). p. 288-295. | - |
item.accessRights | Closed Access | - |
Appears in Collections: | Research publications |
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