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Title: | LEO1 is a partner for Cockayne syndrome protein B (CSB) in response to transcription-blocking DNA damage | Authors: | Tiwari, V Kulikowicz, T WILSON, David Bohr, VA |
Issue Date: | 2021 | Publisher: | OXFORD UNIV PRESS | Source: | Nucleic acids research, 49 (11) , p. 6331 -6346 | Abstract: | Cockayne syndrome (CS) is an autosomal recessive genetic disorder characterized by photosensitivity, developmental defects, neurological abnormalities, and premature aging. Mutations in CSA (ERCC8), CSB (ERCC6), XPB, XPD, XPG, XPF (ERCC4) and ERCC1 can give rise to clinical phenotypes resembling classic CS. Using a yeast two-hybrid (Y2H) screening approach, we identified LEO1 (Phe381Ser568 region) as an interacting protein partner of full-length and C-terminal (Pro1010-Cys1493) CSB in two independent screens. LEO1 is a member of the RNA polymerase associated factor 1 complex (PAF1C) with roles in transcription elongation and chromatin modification. Supportive of the Y2H results, purified, recombinant LEO1 and CSB directly interact in vitro, and the two proteins exist in a common complex within human cells. In addition, fluorescently tagged LEO1 and CSB are both recruited to localized DNA damage sites in human cells. Cell fractionation experiments revealed a transcription-dependent, coordinated association of LEO1 and CSB to chromatin following either UVC irradiation or cisplatin treatment of HEK293T cells, whereas the response to menadione was distinct, suggesting that this collaboration occurs mainly in the context of bulky transcription-blocking lesions. Consistent with a coordinated interaction in DNA repair, LEO1 knockdown or knockout resulted in reduced CSB recruitment to chromatin, increased sensitivity to UVC light and cisplatin damage, and reduced RNA synthesis recovery and slower excision of cyclobutane pyrimidine dimers following UVC irradiation; the absence of CSB resulted in diminished LEO1 recruitment. Our data indicate a reciprocal communication be- tween CSB and LEO1 in the context of transcriptionassociated DNA repair and RNA transcription recovery. | Keywords: | Chromatin;DNA Adducts;DNA Damage;DNA Helicases;DNA Repair Enzymes;HEK293 Cells;HeLa Cells;Humans;Mutagens;Poly-ADP-Ribose Binding Proteins;RNA;Transcription Factors;Transcription, Genetic;DNA Repair | Document URI: | http://hdl.handle.net/1942/36236 | ISSN: | 0305-1048 | e-ISSN: | 1362-4962 | DOI: | 10.1093/nar/gkab458 | ISI #: | 000671550100028 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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ACFrOgA5uflrS-xrNXANMV0-Q66O4REis77iQw6YSg2dfdAhPHbFtgcrKsihr1RfrNQqI-VMGJAfsOjar7im3hD7G7L6GOqH45PlLGGvJgr7CHZJGb33kJBDqrcdJkgNWRiM3ACpc64jigFrENAh.pdf | Peer-reviewed author version | 228.08 kB | Adobe PDF | View/Open |
gkab458.pdf Restricted Access | Published version | 6.79 MB | Adobe PDF | View/Open Request a copy |
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